ID   ZORA_ECOLC              Reviewed;         550 AA.
AC   P0DW01;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Zorya protein ZorA {ECO:0000303|PubMed:29371424};
GN   Name=zorA {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcolC_3888 {ECO:0000312|EMBL:ACA79490.1};
OS   Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS   WDCM 00012 / Crooks).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=481805;
RN   [1] {ECO:0000312|EMBL:ACA79490.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Ingram L., Richardson P.;
RT   "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type II, composed
CC       of ZorA, ZorB and ZorE. Expression of Zorya type II in E.coli (strain
CC       MG1655) confers resistance to phages SECphi7 and T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 170 minutes
CC       after initial infection (PubMed:29371424). ZorA and ZorB probably
CC       assemble in the cell inner membrane and exert their effect there
CC       (Probable). {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:29371424}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the MotA family. {ECO:0000305|PubMed:29371424}.
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DR   EMBL; CP000946; ACA79490.1; -; Genomic_DNA.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..550
FT                   /note="Zorya protein ZorA"
FT                   /id="PRO_0000456339"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   550 AA;  61483 MW;  CE876748953965B7 CRC64;
     MLAQLFEQLF QSIDSTLITN IFIWAVIFVF LSAWWCDKKN IHSKFREYAP TLMGALGILG
     TFIGIIIGLL NFNTESIDTS IPVLLGGLKT AFITSIVGMF FAILFNGMDA FFFANKRSAL
     AENNPESVTP EHIYHELKEQ NQTLTKLVSG INGDSEGSLI AQIKLLRTEI SDSSQAQLAN
     HTHFSNKLWE QLEQFADLMA KGATEQIIDA LRQVIIDFNE NLTEQFGENF KALDASVKKL
     VEWQGNYKTQ IEQMSEQYQQ SVESLVETKT AVAGIWEECK EIPLAMSELR EVLQVNQHQI
     SELSRHLETF VAIRDKATTV LPEIQNKMAE VGELLKSGAA NVSASLEQTS QQILLNADSM
     RVALDEGTEG FRQSVTQTQQ AFASMAHDVS NSSETLTSTL GETITEMKQS GEEFLKSLES
     HSKELHRNME QNTTNVIDMF SKTGEKINHQ LSSNADNMFD SIQTSFDKAG AGLTSQVRES
     IEKFALSINE QLHAFEQATE REMNREMQSL GNALLSISKG FVGNYEKLIK DYQIVMGQLQ
     ALISANKHRG