ID   ZORB_ECO24              Reviewed;         246 AA.
AC   A7ZI09;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Zorya protein ZorB {ECO:0000303|PubMed:29371424};
GN   Name=zorB {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcE24377A_0284 {ECO:0000312|EMBL:ABV17786.1};
OS   Escherichia coli O139:H28 (strain E24377A / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331111;
RN   [1] {ECO:0000312|EMBL:ABV17786.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E24377A / ETEC;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP   ASP-26.
RC   STRAIN=E24377A / ETEC;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type I, composed
CC       of ZorA, ZorB, ZorC and ZorD. Expression of Zorya type I in E.coli
CC       (strain MG1655) confers 10,000-fold resistance to phage SECphi27, 100-
CC       fold resistance to lambda, and 10-fold resistance to T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 2 hours after
CC       initial infection (PubMed:29371424). ZorA and ZorB probably assemble in
CC       the cell inner membrane and exert their effect there (Probable).
CC       {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:29371424}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Zorya system does
CC       not confer resistance to SECphi27 in E.coli.
CC       {ECO:0000269|PubMed:29371424}.
CC   -!- SIMILARITY: Belongs to the MotB family. {ECO:0000305}.
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DR   EMBL; CP000800; ABV17786.1; -; Genomic_DNA.
DR   RefSeq; WP_000467667.1; NC_009801.1.
DR   EnsemblBacteria; ABV17786; ABV17786; EcE24377A_0284.
DR   KEGG; ecw:EcE24377A_0284; -.
DR   HOGENOM; CLU_016890_2_2_6; -.
DR   OMA; EGEKPFW; -.
DR   Proteomes; UP000001122; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.60; -; 1.
DR   InterPro; IPR025713; MotB_N_dom.
DR   InterPro; IPR006665; OmpA-like.
DR   InterPro; IPR036737; OmpA-like_sf.
DR   Pfam; PF13677; MotB_plug; 1.
DR   Pfam; PF00691; OmpA; 1.
DR   SUPFAM; SSF103088; SSF103088; 1.
DR   PROSITE; PS51123; OMPA_2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..246
FT                   /note="Zorya protein ZorB"
FT                   /id="PRO_0000456340"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          86..218
FT                   /note="OmpA-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00473"
FT   MUTAGEN         26
FT                   /note="D->N: No longer resistant to SECphi27."
FT                   /evidence="ECO:0000269|PubMed:29371424"
SQ   SEQUENCE   246 AA;  28126 MW;  559C5E9F91C746DA CRC64;
     MFGNAFGVKK RRSDEAEKPF WISYADLMTA MMVLFLVVMV ASLSSVTQRI QRAEQGEKTR
     GQDISRLCER LELHARNVNK TIVVDCHDNR ISFGEAGRFD HNQFFLNAEG QKALQDVVPL
     VLEASNSEEG KKWFKQIVIE GFTDTDGSYL YNLHLSLQRS EWVMCSLLDS RSPLQKNISA
     EQQLQIRKLF LAGGVSFNNA KESKEASRRV ELRMQFFGLK DKRDKADEVD FPPVVNKEVC
     QLVMPL