ID   ZORB_ECOLC              Reviewed;         235 AA.
AC   P0DW02;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Zorya protein ZorB {ECO:0000303|PubMed:29371424};
GN   Name=zorB {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcolC_3889 {ECO:0000312|EMBL:ACA79491.1};
OS   Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS   WDCM 00012 / Crooks).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=481805;
RN   [1] {ECO:0000312|EMBL:ACA79491.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Ingram L., Richardson P.;
RT   "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type II, composed
CC       of ZorA, ZorB and ZorE. Expression of Zorya type II in E.coli (strain
CC       MG1655) confers resistance to phages SECphi7 and T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 170 minutes
CC       after initial infection (PubMed:29371424). ZorA and ZorB probably
CC       assemble in the cell inner membrane and exert their effect there
CC       (Probable). {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:29371424}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the MotB family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000946; ACA79491.1; -; Genomic_DNA.
DR   PROSITE; PS51123; OMPA_2; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..235
FT                   /note="Zorya protein ZorB"
FT                   /id="PRO_0000456341"
FT   TRANSMEM        25..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          87..225
FT                   /note="OmpA-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00473"
SQ   SEQUENCE   235 AA;  27330 MW;  99C320EBA52B2FE2 CRC64;
     MDKIIGKQLP KKDQDNEHWV SMSDLMAGLM MVFMFISIAY MHYVRIEKEK IKEVAVAYEN
     AQLQIYNALD IEFAKDLQDW DAEIDKQTLE VRFKSPDVLF GLGSTELKPK FKLILDDFFP
     RYLKVLDNYQ EHITEVRIEG HTSTDWTGTT NPDIAYFNNM ALSQGRTRAV LQYVYDIKNI
     ATHQQWVKSK FAAVGYSSAH PILDKTGKED PNRSRRVTFK VVTNAELQIR KIIQE