ID   ZORC_ECO24              Reviewed;         560 AA.
AC   A7ZI08;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 2.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Zorya protein ZorC {ECO:0000303|PubMed:29371424};
GN   Name=zorC {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcE24377A_0283 {ECO:0000312|EMBL:ABV16709.1};
OS   Escherichia coli O139:H28 (strain E24377A / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331111;
RN   [1] {ECO:0000312|EMBL:ABV16709.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E24377A / ETEC;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-400 AND HIS-443.
RC   STRAIN=E24377A / ETEC;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type I, composed
CC       of ZorA, ZorB, ZorC and ZorD. Expression of Zorya type I in E.coli
CC       (strain MG1655) confers 10,000-fold resistance to phage SECphi27, 100-
CC       fold resistance to lambda, and 10-fold resistance to T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 2 hours after
CC       initial infection (PubMed:29371424). ZorA and ZorB probably assemble in
CC       the cell inner membrane and exert their effect there (Probable).
CC       {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Zorya system does
CC       not confer resistance to SECphi27 in E.coli.
CC       {ECO:0000269|PubMed:29371424}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV16709.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:29371424};
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DR   EMBL; CP000800; ABV16709.1; ALT_INIT; Genomic_DNA.
DR   EnsemblBacteria; ABV16709; ABV16709; EcE24377A_0283.
DR   KEGG; ecw:EcE24377A_0283; -.
DR   HOGENOM; CLU_035807_0_0_6; -.
DR   OMA; RTWPLID; -.
DR   Proteomes; UP000001122; Chromosome.
DR   InterPro; IPR028943; EH_Signature_dom.
DR   Pfam; PF15611; EH_Signature; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..560
FT                   /note="Zorya protein ZorC"
FT                   /id="PRO_0000456342"
FT   MUTAGEN         400
FT                   /note="E->A: No longer resistant to SECphi27; when
FT                   associated with A-443."
FT                   /evidence="ECO:0000269|PubMed:29371424"
FT   MUTAGEN         443
FT                   /note="H->A: No longer resistant to SECphi27; when
FT                   associated with A-400."
FT                   /evidence="ECO:0000269|PubMed:29371424"
SQ   SEQUENCE   560 AA;  64903 MW;  DBC31A4750C578AB CRC64;
     MTPALNSLSQ RIAARLSSSQ RDDHYLHNDF HALASAALDM EKRFDKAEKI PLPPQKMRLA
     ALRRLRLAQE LTEREWRMVF YGLADNDPSY PDQPVLLEDD TFFPEVNNAI KKRLETKTLK
     RRDWAALCSS YFAYQNPSPE TNPHWCVLRG HIAQGYMVVK AAIRREKSWM KTIEFYHDIF
     TPQAGGVISR QLLAGESNSL SSLEKIAQIP DSSWLWKRIF TVLLAQLDTL DDPQFLDKIS
     WLLGLAAQWV RFRDDIMTAT LTRYYHSIYR DQAHSALKQA ALEYWDNPQL KSQQNKWHQY
     VSEPVAAMVR GWLAKQDLMH FFELLRGNGD VDQARLHYWL RFANQMGFTR IVMGTDAWQD
     RGSDFVKFRE ENKGRLSYLR GGRNFDNAMI MQINDYLFVE FSGTGNAMYA YRIGHAPFNP
     ESRTLDINIH LKDKGRCVLR LPHTPRAEGY NKVRITGWML KYDDELRQLG IRWMAEEAIK
     FVDKKASSPA SMSDIKIINP LRDTAIQHLV EGSSCIVSDN RQKGGVLSVQ LNTPDDTIER
     ELLRLGFAPV AKEPHRYWIK