ID   ZORE_ECOLC              Reviewed;         367 AA.
AC   P0DW03;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Zorya protein ZorE {ECO:0000303|PubMed:29371424};
DE   AltName: Full=Putative nuclease ZorE {ECO:0000303|PubMed:29371424};
GN   Name=zorE {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcolC_3890 {ECO:0000312|EMBL:ACA79492.1};
OS   Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS   WDCM 00012 / Crooks).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=481805;
RN   [1] {ECO:0000312|EMBL:ACA79492.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Ingram L., Richardson P.;
RT   "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type II, composed
CC       of ZorA, ZorB and ZorE. Expression of Zorya type II in E.coli (strain
CC       MG1655) confers resistance to phages SECphi7 and T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 170 minutes
CC       after initial infection (PubMed:29371424). ZorA and ZorB probably
CC       assemble in the cell inner membrane and exert their effect there. This
CC       may be a nuclease (Probable). {ECO:0000269|PubMed:29371424,
CC       ECO:0000305|PubMed:29371424}.
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DR   EMBL; CP000946; ACA79492.1; -; Genomic_DNA.
PE   4: Predicted;
KW   Antiviral defense.
FT   CHAIN           1..367
FT                   /note="Zorya protein ZorE"
FT                   /id="PRO_0000456343"
SQ   SEQUENCE   367 AA;  42067 MW;  7669489C0F244123 CRC64;
     MKLSIDISEL IQLGKKMLPE GVDFFLDESP IDFDPIDIEL STGKEVSIED LDPGSGLISY
     HGRQVLLYIR DHSGRYDAAI VDGEKGKRFH IAWCRTLDEM RHKNRFERYH ATNRIDGLFE
     IDDGSGRSQD VDLRVCMNCL ERLNYKGSID KQRKREIFKS FSLNEFFSDY STCFRHMPKG
     IYDKTNSGYV ENWKEISKEI REKANYVCND CGVNLSTAKN LCHVHHKNGI KYDNHHENLL
     VLCKDCHRKQ PLHEGIFVTQ AEMAIIQRLR SQQGLLKAES WNEIYDLTDP SVHGDINMMQ
     HKGFQPPVPG LDLQNSEHEI IATVEAAWPG LKIAVNLTPA EVEGWRIYTV GELVKEIQTG
     AFTPAKL