ID   ZOT_VIBCH               Reviewed;         399 AA.
AC   P38442; Q9L7Q6; Q9R3V6;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Zona occludens toxin;
DE   AltName: Full=Zonular occludens toxin;
DE            Short=Zot;
GN   Name=zot; OrderedLocusNames=VC_1458;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Classical Inaba 569B;
RX   PubMed=1730472; DOI=10.1128/iai.60.2.428-434.1992;
RA   Baudry B., Fasano A., Ketley J., Kaper J.B.;
RT   "Cloning of a gene (zot) encoding a new toxin produced by Vibrio
RT   cholerae.";
RL   Infect. Immun. 60:428-434(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KNIH002;
RA   Shin H.J., Park Y.C., Kim Y.C.;
RT   "Cloning and nucleotide sequence analysis of the virulence gene cassette
RT   from Vibrio cholerae KNIH002 isolated in Korea.";
RL   Misainmurhag Hoiji 35:205-210(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O139-Tor Ogawa;
RA   Zhi-Yong H., Wei-Jie Z., Xiang-Fu W.;
RT   "Cloning and Expression of zot gene from Vibrio cholerae.";
RL   Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor 86015 / Serotype O1;
RA   Kan B., Liu Y.Q., Qi G.M., Gao S.Y.;
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [6]
RP   CHARACTERIZATION.
RX   PubMed=2052603; DOI=10.1073/pnas.88.12.5242;
RA   Fasano A., Baudry B., Pumplin D.W., Wasserman S.S., Tall B.D., Ketley J.M.,
RA   Kaper J.B.;
RT   "Vibrio cholerae produces a second enterotoxin, which affects intestinal
RT   tight junctions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:5242-5246(1991).
CC   -!- FUNCTION: Increases the permeability of the small intestine mucosa by
CC       affecting the structure of intercellular tight junctions (zonula
CC       occludens).
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DR   EMBL; M83563; AAA27582.1; -; Genomic_DNA.
DR   EMBL; AF175708; AAD51358.1; -; Genomic_DNA.
DR   EMBL; AF123049; AAD26854.1; -; Genomic_DNA.
DR   EMBL; AF220606; AAF29547.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF94615.1; -; Genomic_DNA.
DR   PIR; A43864; A43864.
DR   PIR; B82197; B82197.
DR   RefSeq; NP_231101.1; NC_002505.1.
DR   RefSeq; WP_000021616.1; NZ_LT906614.1.
DR   AlphaFoldDB; P38442; -.
DR   STRING; 243277.VC_1458; -.
DR   DNASU; 2613964; -.
DR   EnsemblBacteria; AAF94615; AAF94615; VC_1458.
DR   GeneID; 57740121; -.
DR   KEGG; vch:VC_1458; -.
DR   PATRIC; fig|243277.26.peg.1388; -.
DR   eggNOG; COG4128; Bacteria.
DR   HOGENOM; CLU_700097_0_0_6; -.
DR   OMA; PDFAQIP; -.
DR   BioCyc; VCHO:VC1458-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008900; Zot_N.
DR   Pfam; PF05707; Zot; 1.
PE   1: Evidence at protein level;
KW   Enterotoxin; Reference proteome; Toxin; Virulence.
FT   CHAIN           1..399
FT                   /note="Zona occludens toxin"
FT                   /id="PRO_0000066592"
FT   VARIANT         45
FT                   /note="M -> I (in strain: 569B)"
FT   VARIANT         100
FT                   /note="V -> A (in strain: 569B and 86015)"
FT   VARIANT         272
FT                   /note="V -> A (in strain: 569B)"
FT   VARIANT         281
FT                   /note="V -> A (in strain: 569B)"
FT   VARIANT         349
FT                   /note="A -> S (in strain: 86015)"
FT   VARIANT         381
FT                   /note="K -> R (in strain: 86015)"
FT   CONFLICT        386..399
FT                   /note="IKTENDKKGLNSIF -> VKKEKEESIIKSFL (in Ref. 4)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   399 AA;  44903 MW;  3C7424B758176774 CRC64;
     MSIFIHHGAP GSYKTSGALW LRLLPAIKSG RHIITNVRGL NLERMAKYLK MDVSDISIEF
     IDTDHPDGRL TMARFWHWAR KDAFLFIDEC GRIWPPRLTV TNLKALDTPP DLVAEDRPES
     FEVAFDMHRH HGWDICLTTP NIAKVHNMIR EAAEIGYRHF NRATVGLGAK FTLTTHDAAN
     SGQMDSHALT RQVKKIPSPI FKMYASTTTG KARDTMAGTA LWKDRKILFL FGMVFLMFSY
     SFYGLHDNPI FTGGNDATIE SEQSEPQSKA TVGNAVGSKA VAPASFGFCI GRLCVQDGFV
     TVGDERYRLV DNLDIPYRGL WATGHHIYKD TLTVFFETES GSVPTELFAS SYRYKVLPLP
     DFNHFVVFDT FAAQALWVEV KRGLPIKTEN DKKGLNSIF