ZPBP2_MOUSE
ID ZPBP2_MOUSE Reviewed; 326 AA.
AC Q6X786; A2A4Y0; Q6X785; Q9DA91;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Zona pellucida-binding protein 2;
DE Flags: Precursor;
GN Name=Zpbp2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57W;
RA Lin Y.-N., Yan W., Burns K.H., Matzuk M.M.;
RT "Characterization of zona pellucida binding protein 2 (ZPBP2), a paralog of
RT ZPBP1.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP GLYCOSYLATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17664285; DOI=10.1128/mcb.01029-07;
RA Lin Y.N., Roy A., Yan W., Burns K.H., Matzuk M.M.;
RT "Loss of zona pellucida binding proteins in the acrosomal matrix disrupts
RT acrosome biogenesis and sperm morphogenesis.";
RL Mol. Cell. Biol. 27:6794-6805(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Is implicated in sperm-oocyte interaction during
CC fertilization. {ECO:0000269|PubMed:17664285}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:17664285}. Secreted {ECO:0000250}. Note=Released
CC after the acrosomal reaction. {ECO:0000269|PubMed:17664285}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6X786-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6X786-2; Sequence=VSP_011685;
CC Name=3;
CC IsoId=Q6X786-3; Sequence=VSP_011684;
CC -!- TISSUE SPECIFICITY: Expressed specifically in male germ cells.
CC {ECO:0000269|PubMed:17664285}.
CC -!- DEVELOPMENTAL STAGE: Expressed from the mid-pachytene spermatocyte
CC stage to the early elongating spermatid stage.
CC {ECO:0000269|PubMed:17664285}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:17664285}.
CC -!- DISRUPTION PHENOTYPE: Male mice are subfertile with sperm showing
CC reduced ability to penetrate zona pellucida and displaying subtle
CC morphological deformation, including shortened apical hook, smaller
CC apical angle and bulges in acrosome region.
CC {ECO:0000269|PubMed:17664285}.
CC -!- SIMILARITY: Belongs to the zona pellucida-binding protein Sp38 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY251601; AAP83948.1; -; mRNA.
DR EMBL; AY251602; AAP83949.1; -; mRNA.
DR EMBL; AK006058; BAB24388.1; -; mRNA.
DR EMBL; AL591125; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS25353.1; -. [Q6X786-1]
DR CCDS; CCDS25354.1; -. [Q6X786-2]
DR CCDS; CCDS48903.1; -. [Q6X786-3]
DR RefSeq; NP_001159966.1; NM_001166494.1. [Q6X786-3]
DR RefSeq; NP_001159967.1; NM_001166495.1. [Q6X786-3]
DR RefSeq; NP_081337.2; NM_027061.2. [Q6X786-1]
DR AlphaFoldDB; Q6X786; -.
DR STRING; 10090.ENSMUSP00000017339; -.
DR GlyGen; Q6X786; 3 sites.
DR PaxDb; Q6X786; -.
DR PRIDE; Q6X786; -.
DR ProteomicsDB; 275156; -. [Q6X786-1]
DR ProteomicsDB; 275157; -. [Q6X786-2]
DR ProteomicsDB; 275158; -. [Q6X786-3]
DR Antibodypedia; 16341; 67 antibodies from 17 providers.
DR Ensembl; ENSMUST00000017339; ENSMUSP00000017339; ENSMUSG00000017195. [Q6X786-1]
DR Ensembl; ENSMUST00000081033; ENSMUSP00000079822; ENSMUSG00000017195. [Q6X786-2]
DR Ensembl; ENSMUST00000107509; ENSMUSP00000103133; ENSMUSG00000017195. [Q6X786-3]
DR Ensembl; ENSMUST00000107511; ENSMUSP00000103135; ENSMUSG00000017195. [Q6X786-3]
DR GeneID; 69376; -.
DR KEGG; mmu:69376; -.
DR UCSC; uc007lgo.2; mouse. [Q6X786-1]
DR CTD; 124626; -.
DR MGI; MGI:1916626; Zpbp2.
DR VEuPathDB; HostDB:ENSMUSG00000017195; -.
DR eggNOG; ENOG502R75S; Eukaryota.
DR GeneTree; ENSGT00520000055647; -.
DR HOGENOM; CLU_056016_1_0_1; -.
DR InParanoid; Q6X786; -.
DR OMA; CHFVKIP; -.
DR PhylomeDB; Q6X786; -.
DR TreeFam; TF335677; -.
DR BioGRID-ORCS; 69376; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Zpbp2; mouse.
DR PRO; PR:Q6X786; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q6X786; protein.
DR Bgee; ENSMUSG00000017195; Expressed in spermatid and 35 other tissues.
DR ExpressionAtlas; Q6X786; baseline and differential.
DR Genevisible; Q6X786; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:MGI.
DR GO; GO:0044297; C:cell body; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0002199; C:zona pellucida receptor complex; IDA:MGI.
DR GO; GO:0001675; P:acrosome assembly; IMP:MGI.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IDA:MGI.
DR GO; GO:0032922; P:circadian regulation of gene expression; IMP:MGI.
DR GO; GO:0046466; P:membrane lipid catabolic process; IMP:MGI.
DR GO; GO:0002638; P:negative regulation of immunoglobulin production; IMP:MGI.
DR GO; GO:0006665; P:sphingolipid metabolic process; IMP:MGI.
DR InterPro; IPR010857; Sp38-bd.
DR PANTHER; PTHR15443; PTHR15443; 1.
DR Pfam; PF07354; Sp38; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..326
FT /note="Zona pellucida-binding protein 2"
FT /id="PRO_0000041602"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 220
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..54
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_011684"
FT VAR_SEQ 135..207
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_011685"
FT CONFLICT 224
FT /note="D -> E (in Ref. 1; AAP83948/AAP83949 and 2;
FT BAB24388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 37116 MW; 767D22448418D847 CRC64;
MLAWALLYAV LWSLAGVGSQ RSSLFNIKGF VYGTVGHPVK IYVKLHQASP VLICMDIDRA
NKETVDPTYL WTGPNENTLK GNSQINITNT GELVLKDFLE PLSGLYSCML SYKTIKAETQ
EETMIKKKYD FLVFAYREPD YSYHMAVRFT TGSCVGRHND LLFRVLKKIL DNLISDLLCH
VIEPSYKCHF VKLPERDFLY ELFIAFQVNP FAPGWRSLCN RSADCEDITN HNVLKARDRM
EEFFRKQAHI LHHHFNRTVP AMHFVDHSFQ VTRIDNCRPG FGKNEGLHSN CATCCVVCSP
GTFSPDVDVT CQICVSVHIY GAKACP
