ID   ZPBP2_RAT               Reviewed;         326 AA.
AC   Q6X782; Q6X781;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Zona pellucida-binding protein 2;
DE   Flags: Precursor;
GN   Name=Zpbp2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=Sprague-Dawley;
RA   Lin Y.-N., Yan W., Burns K.H., Matzuk M.M.;
RT   "Characterization of zona pellucida binding protein 2 (ZPBP2), a paralog of
RT   ZPBP1.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Is implicated in sperm-oocyte interaction during
CC       fertilization. {ECO:0000250|UniProtKB:Q6X786}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Cytoplasmic vesicle,
CC       secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q6X786}.
CC       Note=Released after the acrosomal reaction.
CC       {ECO:0000250|UniProtKB:Q6X786}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6X782-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6X782-2; Sequence=VSP_011686;
CC   -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q6X786}.
CC   -!- SIMILARITY: Belongs to the zona pellucida-binding protein Sp38 family.
CC       {ECO:0000305}.
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DR   EMBL; AY251605; AAP83952.1; -; mRNA.
DR   EMBL; AY251606; AAP83953.1; -; mRNA.
DR   EMBL; BC082007; AAH82007.1; -; mRNA.
DR   RefSeq; NP_001007012.1; NM_001007011.1. [Q6X782-1]
DR   AlphaFoldDB; Q6X782; -.
DR   STRING; 10116.ENSRNOP00000048783; -.
DR   GlyGen; Q6X782; 3 sites.
DR   PaxDb; Q6X782; -.
DR   PRIDE; Q6X782; -.
DR   Ensembl; ENSRNOT00000009535; ENSRNOP00000009535; ENSRNOG00000007255. [Q6X782-2]
DR   Ensembl; ENSRNOT00000048647; ENSRNOP00000048783; ENSRNOG00000007255. [Q6X782-1]
DR   GeneID; 363676; -.
DR   KEGG; rno:363676; -.
DR   UCSC; RGD:1549726; rat. [Q6X782-1]
DR   CTD; 124626; -.
DR   RGD; 1549726; Zpbp2.
DR   eggNOG; ENOG502R75S; Eukaryota.
DR   GeneTree; ENSGT00520000055647; -.
DR   HOGENOM; CLU_056016_1_0_1; -.
DR   InParanoid; Q6X782; -.
DR   OMA; CHFVKIP; -.
DR   OrthoDB; 1069241at2759; -.
DR   PhylomeDB; Q6X782; -.
DR   TreeFam; TF335677; -.
DR   PRO; PR:Q6X782; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000007255; Expressed in testis and 17 other tissues.
DR   Genevisible; Q6X782; RN.
DR   GO; GO:0001669; C:acrosomal vesicle; ISO:RGD.
DR   GO; GO:0044297; C:cell body; ISO:RGD.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0002199; C:zona pellucida receptor complex; ISO:RGD.
DR   GO; GO:0001675; P:acrosome assembly; ISO:RGD.
DR   GO; GO:0007339; P:binding of sperm to zona pellucida; ISO:RGD.
DR   GO; GO:0032922; P:circadian regulation of gene expression; ISO:RGD.
DR   GO; GO:0046466; P:membrane lipid catabolic process; ISO:RGD.
DR   GO; GO:0002638; P:negative regulation of immunoglobulin production; ISO:RGD.
DR   GO; GO:0006665; P:sphingolipid metabolic process; ISO:RGD.
DR   InterPro; IPR010857; Sp38-bd.
DR   PANTHER; PTHR15443; PTHR15443; 1.
DR   Pfam; PF07354; Sp38; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasmic vesicle; Glycoprotein;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..326
FT                   /note="Zona pellucida-binding protein 2"
FT                   /id="PRO_0000041603"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        220
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        256
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         17..38
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_011686"
SQ   SEQUENCE   326 AA;  36795 MW;  44C77C38421A13C4 CRC64;
     MLAWALLSAV LWSLAGVGSA RSSLFSNEGF VYGTVGHPVK IYVKLHQTSP VLVCMDIDRA
     SKETVDPIYL WIGPNENTLT GSSQINITNI GELVLKDFME SLSGHYSCTL SYKIVKAETQ
     EETSLKKKYD FLVFAYREPD YSYRMAVRFT TKSCVGRYND LLFRLLKKIL DNLISDLLCH
     VIEPSYKCHS VKIPERDFVY ELFVAFQVNP FAPGWKSMCN SSMDCEDVTN HNILKARDRI
     EEFFRSQAYI LHHHFNITVP AMHFVDHSFQ VTRIDNCRPG FGKNEGLHSN CASCCVVCSP
     GTFSPDMDVT CQTCVSAHVY GAKACP