ZPLD1_MOUSE
ID ZPLD1_MOUSE Reviewed; 415 AA.
AC Q8BGZ8; Q14BA1;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Zona pellucida-like domain-containing protein 1;
DE Short=ZP domain-containing protein 1;
DE AltName: Full=Cupulin {ECO:0000250|UniProtKB:C0H9B6};
DE Contains:
DE RecName: Full=Zona pellucida-like domain-containing protein 1, secreted form {ECO:0000250|UniProtKB:C0H9B6};
DE Flags: Precursor;
GN Name=Zpld1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Head, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Glycoprotein which is a component of the gelatinous
CC extracellular matrix in the cupulae of the vestibular organ.
CC {ECO:0000250|UniProtKB:C0H9B6}.
CC -!- SUBCELLULAR LOCATION: [Zona pellucida-like domain-containing protein
CC 1]: Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:C0H9B6};
CC Single-pass type I membrane protein {ECO:0000255}.
CC -!- SUBCELLULAR LOCATION: [Zona pellucida-like domain-containing protein 1,
CC secreted form]: Secreted, extracellular space, extracellular matrix
CC {ECO:0000250|UniProtKB:C0H9B6}.
CC -!- PTM: Proteolytically cleaved before the transmembrane segment to yield
CC the secreted form found in the extracellular matrix of the cupula.
CC {ECO:0000250|UniProtKB:C0H9B6}.
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DR EMBL; AK034628; BAC28777.1; -; mRNA.
DR EMBL; AK047981; BAC33205.1; -; mRNA.
DR EMBL; AK052599; BAC35056.1; -; mRNA.
DR EMBL; BC116254; AAI16255.1; -; mRNA.
DR EMBL; BC116255; AAI16256.1; -; mRNA.
DR CCDS; CCDS28215.1; -.
DR RefSeq; NP_848835.1; NM_178720.4.
DR RefSeq; XP_006522195.1; XM_006522132.2.
DR AlphaFoldDB; Q8BGZ8; -.
DR SMR; Q8BGZ8; -.
DR STRING; 10090.ENSMUSP00000119774; -.
DR GlyGen; Q8BGZ8; 1 site.
DR iPTMnet; Q8BGZ8; -.
DR PhosphoSitePlus; Q8BGZ8; -.
DR jPOST; Q8BGZ8; -.
DR PaxDb; Q8BGZ8; -.
DR PRIDE; Q8BGZ8; -.
DR Antibodypedia; 32313; 95 antibodies from 18 providers.
DR Ensembl; ENSMUST00000036412; ENSMUSP00000049012; ENSMUSG00000064310.
DR Ensembl; ENSMUST00000143914; ENSMUSP00000119774; ENSMUSG00000064310.
DR GeneID; 239852; -.
DR KEGG; mmu:239852; -.
DR UCSC; uc007zlk.1; mouse.
DR CTD; 131368; -.
DR MGI; MGI:2443415; Zpld1.
DR VEuPathDB; HostDB:ENSMUSG00000064310; -.
DR eggNOG; ENOG502QQQ1; Eukaryota.
DR GeneTree; ENSGT00940000156527; -.
DR HOGENOM; CLU_045259_2_0_1; -.
DR InParanoid; Q8BGZ8; -.
DR OMA; PFLMPVC; -.
DR OrthoDB; 692009at2759; -.
DR PhylomeDB; Q8BGZ8; -.
DR TreeFam; TF330284; -.
DR BioGRID-ORCS; 239852; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Zpld1; mouse.
DR PRO; PR:Q8BGZ8; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BGZ8; protein.
DR Bgee; ENSMUSG00000064310; Expressed in proximal tubule and 23 other tissues.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005201; F:extracellular matrix structural constituent; IBA:GO_Central.
DR GO; GO:0060005; P:vestibular reflex; IMP:MGI.
DR Gene3D; 2.60.40.4100; -; 1.
DR InterPro; IPR042235; ZP-C.
DR InterPro; IPR001507; ZP_dom.
DR Pfam; PF00100; Zona_pellucida; 1.
DR SMART; SM00241; ZP; 1.
DR PROSITE; PS51034; ZP_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Membrane; Reference proteome; Secreted; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..415
FT /note="Zona pellucida-like domain-containing protein 1"
FT /id="PRO_0000307286"
FT CHAIN 20..319
FT /note="Zona pellucida-like domain-containing protein 1,
FT secreted form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000441816"
FT TOPO_DOM 20..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 43..320
FT /note="ZP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00375"
FT SITE 319..320
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:P10761"
FT CARBOHYD 164
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 44..155
FT /evidence="ECO:0000250|UniProtKB:P10761"
FT DISULFID 79..104
FT /evidence="ECO:0000250|UniProtKB:P10761"
FT DISULFID 235..296
FT /evidence="ECO:0000250|UniProtKB:P10761"
FT DISULFID 255..313
FT /evidence="ECO:0000250|UniProtKB:P10761"
FT CONFLICT 358
FT /note="G -> V (in Ref. 2; AAI16255)"
FT /evidence="ECO:0000305"
FT CONFLICT 408
FT /note="G -> A (in Ref. 2; AAI16255)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 45448 MW; 5ED1871A4E257CA1 CRC64;
MERVWLLFLL AIRVSPGSAQ FNSYNCDANL HSRFPAERDI SVYCGVQAIT MKINFCTVLF
SGYSETDLAL NGRHGDSHCR GFINNNTFPA VVIFIINLST LEGCGNNLVV STIPGVGASG
NATTVQIGNI SGYIDTPDPP AVISYLPGLL YKFSCSYPLE YLVNNTQLAS SSAAISVREN
NGTFVSTLNL LLYNDSTYRE QLIIPSIGLP LKTKVFAAVQ ATNLDGRWNV LMDYCYTTPS
GNPNDDTRYD LFLSCDKDPQ TTVIENGRSQ RGRFSFEVFR FVKHKNQKMS TVFLHCLTKL
CRADDCPLLM PICGNRKRRD AQSWTTWAPQ STSGNAVLSA GPIITRSDET PTNNSQLGSL
SVPPFQLNAV TSSLISGMVI LGVLCFSLLL CSLALLHRKG STSLVLNGVR NPVFE
