ZPR1_YEAST
ID ZPR1_YEAST Reviewed; 486 AA.
AC P53303; D6VUZ4; Q02807; Q02808;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Zinc finger protein ZPR1;
GN Name=ZPR1; OrderedLocusNames=YGR211W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9852145; DOI=10.1083/jcb.143.6.1471;
RA Gangwani L., Mikrut M., Galcheva-Gargova Z., Davis R.J.;
RT "Interaction of ZPR1 with translation elongation factor-1alpha in
RT proliferating cells.";
RL J. Cell Biol. 143:1471-1484(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-459.
RC STRAIN=S288c / GRF88;
RA Song J.M., Cheung E., Rabinowitz J.C.;
RT "Analysis of the 15.6-kb fragment encompassing the ADE3 gene.";
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-407, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBUNIT: Binds to the elongation factor 1-alpha.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocates to the
CC nucleus after nutrient stimulation.
CC -!- MISCELLANEOUS: Present with 39900 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ZPR1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA85586.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA85587.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF019769; AAC33516.1; -; Genomic_DNA.
DR EMBL; Z72996; CAA97238.1; -; Genomic_DNA.
DR EMBL; U40843; AAA85586.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U40843; AAA85587.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006941; DAA08305.1; -; Genomic_DNA.
DR PIR; S64534; S64534.
DR RefSeq; NP_011727.3; NM_001181340.3.
DR AlphaFoldDB; P53303; -.
DR SMR; P53303; -.
DR BioGRID; 33464; 277.
DR DIP; DIP-4419N; -.
DR IntAct; P53303; 7.
DR MINT; P53303; -.
DR STRING; 4932.YGR211W; -.
DR iPTMnet; P53303; -.
DR MaxQB; P53303; -.
DR PaxDb; P53303; -.
DR PRIDE; P53303; -.
DR EnsemblFungi; YGR211W_mRNA; YGR211W; YGR211W.
DR GeneID; 853125; -.
DR KEGG; sce:YGR211W; -.
DR SGD; S000003443; ZPR1.
DR VEuPathDB; FungiDB:YGR211W; -.
DR eggNOG; KOG2703; Eukaryota.
DR GeneTree; ENSGT00390000005306; -.
DR HOGENOM; CLU_024138_5_0_1; -.
DR InParanoid; P53303; -.
DR OMA; PVQSQKG; -.
DR BioCyc; YEAST:G3O-30893-MON; -.
DR PRO; PR:P53303; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53303; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
DR GO; GO:0009749; P:response to glucose; IDA:UniProtKB.
DR Gene3D; 2.20.25.420; -; 2.
DR Gene3D; 2.60.120.1040; -; 2.
DR InterPro; IPR004457; Znf_ZPR1.
DR InterPro; IPR040141; ZPR1.
DR InterPro; IPR042451; ZPR1_A/B_dom.
DR InterPro; IPR042452; ZPR1_Znf1/2.
DR PANTHER; PTHR10876; PTHR10876; 1.
DR Pfam; PF03367; zf-ZPR1; 2.
DR SMART; SM00709; Zpr1; 2.
DR TIGRFAMs; TIGR00310; ZPR1_znf; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Zinc; Zinc-finger.
FT CHAIN 1..486
FT /note="Zinc finger protein ZPR1"
FT /id="PRO_0000119040"
FT ZN_FING 54..86
FT /note="C4-type 1"
FT ZN_FING 295..327
FT /note="C4-type 2"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..28
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 407
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 486 AA; 55072 MW; F523AF8AB5B8ACA0 CRC64;
MSEQKEDLFK PVGEAAAEVE DESIAEQNKA NDGVKLTGAQ DAMGHPVQEI ESLCMNCGKN
GTTRLLLTSI PYFREIIIMS FDCPHCGFKN CEIQPASQIQ EKGSRYVLKV ECREDFNRQV
IKSETATCKF VELDIEIPAK RGQLTTVEGL LSEMIDDLSQ DQEMRKSIDE ALYKKIDDFI
QKVKSYINCE PNTIPITFIL DDPAGNSWIE YKPGEPQHKW SHTQYVRTDE QNVQVGIITR
DQLEQRRQEQ LKQLANRERN PSESVKVGSA NPQFLSDATD IENFNNEVQT FRASCPSCTQ
ECETHMKPVN IPHFKEVIIM STVCDHCGYK SNEVKTGGAI PDKGRRITLY CDDAADLSRD
ILKSETCSMV IPELHLDIQE GTLGGRFTTL EGLLRQVYEE LESRIFTQTS DSMDEATKAR
WVEFFAKLKE AIAGKVKFTV IMEDPLAGSY IQNVYAPDPD PNMTIEDYER TKEQNEDLGL
SDIKVE
