ZPT71_CAEEL
ID ZPT71_CAEEL Reviewed; 393 AA.
AC Q9XUC4;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Zinc transporter zipt-7.1 {ECO:0000305};
DE AltName: Full=Histidine-rich membrane protein KE4 homolog 1 {ECO:0000305};
GN Name=zipt-7.1 {ECO:0000303|PubMed:29879108, ECO:0000312|WormBase:T28F3.3};
GN Synonyms=hke-4.1 {ECO:0000312|WormBase:T28F3.3};
GN ORFNames=T28F3.3 {ECO:0000312|WormBase:T28F3.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP INDUCTION.
RX DOI=10.1093/icb/45.1.61;
RA Novillo A., Won S.-J., Li C., Callard I.P.;
RT "Changes in nuclear receptor and vitellogenin gene expression in response
RT to steroids and heavy metal in Caenorhabditis elegans.";
RL Integr. Comp. Biol. 45:61-71(2005).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF GLY-266.
RX PubMed=29879108; DOI=10.1371/journal.pbio.2005069;
RA Zhao Y., Tan C.H., Krauchunas A., Scharf A., Dietrich N., Warnhoff K.,
RA Yuan Z., Druzhinina M., Gu S.G., Miao L., Singson A., Ellis R.E.,
RA Kornfeld K.;
RT "The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes.";
RL PLoS Biol. 16:E2005069-E2005069(2018).
CC -!- FUNCTION: Zinc transporter which regulates intracellular zinc levels
CC (PubMed:29879108). Required for spermatogenesis in both hermaphrodites
CC and males where it resides in an inactive form in immature sperm,
CC spermatids, but is likely activated in response to reduced spe-4 and
CC spe-6 function (PubMed:29879108). Upon activation, mediates the release
CC of zinc from internal stores in spermatids into the cytoplasm
CC (PubMed:29879108). The resulting increase in cytoplasmic zinc levels
CC promotes spermatid activation and subsequent differentiation into
CC mature motile sperm that are capable of fertilization
CC (PubMed:29879108). {ECO:0000269|PubMed:29879108}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:29879108}; Multi-
CC pass membrane protein {ECO:0000305}. Note=Localizes to membranous
CC organelles. {ECO:0000269|PubMed:29879108}.
CC -!- TISSUE SPECIFICITY: Highly expressed in developing spermatids (at the
CC protein level) (PubMed:29879108). Expressed in the germline
CC (PubMed:29879108). {ECO:0000269|PubMed:29879108}.
CC -!- INDUCTION: By cholesterol. {ECO:0000269|Ref.2}.
CC -!- DISRUPTION PHENOTYPE: Reduced brood size, but the majority of
CC hermaphrodites are sterile and lay a large number of unfertilized
CC oocytes (PubMed:29879108). Defective spermatid activation
CC (PubMed:29879108). Double knockout with spe-4 results in failed
CC spermatocyte division (PubMed:29879108). Double knockout with spe-6
CC results in suppression of the premature spermatid activation phenotype
CC of the single spe-6 (hc163) mutant (PubMed:29879108).
CC {ECO:0000269|PubMed:29879108}.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC KE4/Catsup subfamily. {ECO:0000305}.
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DR EMBL; BX284604; CAB05297.2; -; Genomic_DNA.
DR PIR; T25420; T25420.
DR RefSeq; NP_503070.2; NM_070669.3.
DR AlphaFoldDB; Q9XUC4; -.
DR BioGRID; 43585; 1.
DR STRING; 6239.T28F3.3; -.
DR TCDB; 2.A.5.4.18; the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.
DR PaxDb; Q9XUC4; -.
DR EnsemblMetazoa; T28F3.3.1; T28F3.3.1; WBGene00044067.
DR EnsemblMetazoa; T28F3.3.2; T28F3.3.2; WBGene00044067.
DR EnsemblMetazoa; T28F3.3.3; T28F3.3.3; WBGene00044067.
DR GeneID; 178509; -.
DR KEGG; cel:CELE_T28F3.3; -.
DR CTD; 178509; -.
DR WormBase; T28F3.3; CE36329; WBGene00044067; zipt-7.1.
DR eggNOG; KOG2693; Eukaryota.
DR GeneTree; ENSGT00940000160076; -.
DR HOGENOM; CLU_015114_0_1_1; -.
DR InParanoid; Q9XUC4; -.
DR OMA; IKGGHCH; -.
DR OrthoDB; 657777at2759; -.
DR PhylomeDB; Q9XUC4; -.
DR PRO; PR:Q9XUC4; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00044067; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IMP:UniProtKB.
DR GO; GO:0090727; P:positive regulation of brood size; IMP:UniProtKB.
DR GO; GO:1905516; P:positive regulation of fertilization; IMP:UniProtKB.
DR GO; GO:0046662; P:regulation of oviposition; IMP:UniProtKB.
DR GO; GO:0048515; P:spermatid differentiation; IMP:UniProtKB.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR003689; ZIP.
DR Pfam; PF02535; Zip; 1.
PE 1: Evidence at protein level;
KW Differentiation; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Spermatogenesis; Transmembrane; Transmembrane helix; Transport; Zinc;
KW Zinc transport.
FT CHAIN 1..393
FT /note="Zinc transporter zipt-7.1"
FT /id="PRO_0000213696"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 142..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 66
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 152
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 362
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 266
FT /note="G->E: In as42; defects in egg-laying associated with
FT embryonic hatching within the mother (also called the bag
FT of worms phenotype)."
FT /evidence="ECO:0000269|PubMed:29879108"
SQ SEQUENCE 393 AA; 43324 MW; 27ABE48FB79A92F9 CRC64;
MRLQLVALLF ALVFNAFSHE HSHEHHHEGD GSEILTKVGW NDHSEELHDH HEHDHDHHDE
QLIRKNHTSH REIQHSRLST LKVWVFSLSA VVGISLAPCT LLFFIPAQHA NGPFLKILLA
FGAGGLLGDA LLHIIPHSLS PHDHSHDHHD HNHSHKEHDH SHDHSNQLRV GTFVIAGILV
FMMVEQLVRI IKGGHCHSHE NGHIVADEHR HLNEHDHEHS EEKKQQVEGL KDVKASAYLN
LVADFVHNVT DGLAIGASFS AGNTLGWITT LTVLLHELPH EVGDFAILVQ SGFSKYQAIR
LQAVTALGAI TGCVFSLLVS NPGSLNNDAD TSAIMPFTAG GFIYIATVSV VPELLESGDH
NNLSKVAKMA QSLVHVLAIC MGVGMMYIVS LVE
