ID   ZRAR_KLEOX              Reviewed;         443 AA.
AC   Q9APD9;
DT   03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Transcriptional regulatory protein ZraR;
GN   Name=zraR; Synonyms=hydG;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=11243806; DOI=10.1006/jmbi.2000.4451;
RA   Leonhartsberger S., Huber A., Lottspeich F., Boeck A.;
RT   "The hydH/G genes from Escherichia coli code for a zinc and lead responsive
RT   two-component regulatory system.";
RL   J. Mol. Biol. 307:93-105(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system ZraS/ZraR. When
CC       activated by ZraS it acts in conjunction with sigma-54 to regulate the
CC       expression of zraP. Positively autoregulates the expression of the
CC       zraSR operon (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by ZraS. {ECO:0000305}.
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DR   EMBL; AF305914; AAG59807.1; -; Genomic_DNA.
DR   RefSeq; WP_004870527.1; NZ_LR607357.1.
DR   AlphaFoldDB; Q9APD9; -.
DR   SMR; Q9APD9; -.
DR   PATRIC; fig|571.110.peg.238; -.
DR   OrthoDB; 123059at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Cytoplasm; DNA-binding; Nucleotide-binding;
KW   Phosphoprotein; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..443
FT                   /note="Transcriptional regulatory protein ZraR"
FT                   /id="PRO_0000081281"
FT   DOMAIN          7..121
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          141..370
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        423..442
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         169..176
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         232..241
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   MOD_RES         56
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   443 AA;  48900 MW;  EEC0A6C8D375A4DF CRC64;
     MSGEQVDILV VDDDISHCTI LQALLRGWGY RVALANNGLQ ALEKVREKVF DLVLCDIRMA
     EMDGIETLKE IKTFNPSIPV LIMTAYSSVD TAVEALKSGA LDYLIKPLDF DKLQLTLSEA
     LAHTRLSESP VTETPAAQFG MVGDSPAMRA LLNNITLVAP SDATVLIHGE SGTGKELVAR
     ALHASSARSR RPLVILNCAA LNESLLESEL FGHEKGAFTG ADKRREGRFV EADGGTLFLD
     EIGDISPLMQ VRLLRAIQER EVQRVGSNQT LSVDVRLIAA THRDLAEEVS AGRFRQDLYY
     RLNVVTIDMP PLRHRREDIP PLARYFLQRY AERNRKAVQG FTPQAMDLLI HYAWPGNIRE
     LENAVERAVV LLTGEYISER ELPLAITGTP VADAPHGDDS IQPLVEVEKE AILAALERTG
     GNKTEAARRL GITRKTLLAK LSR