ZRAR_SALTY
ID ZRAR_SALTY Reviewed; 441 AA.
AC P25852; Q9L9H8;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Transcriptional regulatory protein ZraR;
GN Name=zraR; Synonyms=hydG; OrderedLocusNames=STM4174; ORFNames=STMF1.27;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1756170; DOI=10.1016/0167-4781(91)90224-a;
RA Chopra A.K., Peterson J.W., Prasad R.;
RT "Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from
RT Salmonella typhimurium.";
RL Biochim. Biophys. Acta 1129:115-118(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Member of the two-component regulatory system ZraS/ZraR. When
CC activated by ZraS it acts in conjunction with sigma-54 to regulate the
CC expression of zraP. Positively autoregulates the expression of the
CC zraSR operon (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by ZraS. {ECO:0000250}.
CC -!- CAUTION: Was originally thought to be involved in the regulation of the
CC labile hydrogenase activity. {ECO:0000305|PubMed:1756170}.
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DR EMBL; M64988; AAA27149.1; -; mRNA.
DR EMBL; AF170176; AAF33506.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL23002.1; -; Genomic_DNA.
DR PIR; S19606; S19606.
DR RefSeq; NP_463043.1; NC_003197.2.
DR RefSeq; WP_000617942.1; NC_003197.2.
DR PDB; 1OJL; X-ray; 3.00 A; A/B/C/D/E/F=141-441.
DR PDBsum; 1OJL; -.
DR AlphaFoldDB; P25852; -.
DR SMR; P25852; -.
DR STRING; 99287.STM4174; -.
DR PaxDb; P25852; -.
DR EnsemblBacteria; AAL23002; AAL23002; STM4174.
DR GeneID; 1255700; -.
DR KEGG; stm:STM4174; -.
DR PATRIC; fig|99287.12.peg.4388; -.
DR HOGENOM; CLU_000445_0_6_6; -.
DR OMA; MPISMQV; -.
DR PhylomeDB; P25852; -.
DR BioCyc; SENT99287:STM4174-MON; -.
DR EvolutionaryTrace; P25852; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; ATP-binding; Cytoplasm; DNA-binding;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..441
FT /note="Transcriptional regulatory protein ZraR"
FT /id="PRO_0000081283"
FT DOMAIN 7..121
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 141..370
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 421..440
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 169..176
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 232..241
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT MOD_RES 56
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 159
FT /note="A -> R (in Ref. 1; AAA27149)"
FT /evidence="ECO:0000305"
FT HELIX 146..158
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 165..169
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 175..185
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 189..191
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 203..210
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 228..232
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 235..241
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 247..258
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 259..261
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 276..283
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 285..291
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 296..302
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 303..308
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 313..318
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 319..333
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 343..351
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 357..371
FT /evidence="ECO:0007829|PDB:1OJL"
FT STRAND 374..377
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 379..381
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 384..386
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 402..415
FT /evidence="ECO:0007829|PDB:1OJL"
FT TURN 416..419
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 421..428
FT /evidence="ECO:0007829|PDB:1OJL"
FT HELIX 432..438
FT /evidence="ECO:0007829|PDB:1OJL"
SQ SEQUENCE 441 AA; 48590 MW; 5753C4E54F8C52F5 CRC64;
MIRGKIDILV VDDDVSHCTI LQALLRGWGY NVALAYSGHD ALAQVREKVF DLVLCDVRMA
EMDGIATLKE IKALNPAIPI LIMTAFSSVE TAVEALKAGA LDYLIKPLDF DRLQETLEKA
LAHTRETGAE LPSASAAQFG MIGSSPAMQH LLNEIAMVAP SDATVLIHGD SGTGKELVAR
ALHACSARSD RPLVTLNCAA LNESLLESEL FGHEKGAFTG ADKRREGRFV EADGGTLFLD
EIGDISPLMQ VRLLRAIQER EVQRVGSNQT ISVDVRLIAA THRDLAEEVS AGRFRQDLYY
RLNVVAIEMP SLRQRREDIP LLADHFLRRF AERNRKVVKG FTPQAMDLLI HYDWPGNIRE
LENAIERAVV LLTGEYISER ELPLAIAATP IKTEYSGEIQ PLVDVEKEVI LAALEKTGGN
KTEAARQLGI TRKTLLAKLS R
