ID   ZRAS_SALTY              Reviewed;         465 AA.
AC   P37461; Q9L9H9;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   13-DEC-2001, sequence version 2.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=Sensor protein ZraS;
DE            EC=2.7.13.3;
GN   Name=zraS; Synonyms=hydH; OrderedLocusNames=STM4173; ORFNames=STMF1.26;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-465.
RX   PubMed=1756170; DOI=10.1016/0167-4781(91)90224-a;
RA   Chopra A.K., Peterson J.W., Prasad R.;
RT   "Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from
RT   Salmonella typhimurium.";
RL   Biochim. Biophys. Acta 1129:115-118(1991).
CC   -!- FUNCTION: Member of the two-component regulatory system ZraS/ZraR. May
CC       function as a membrane-associated protein kinase that phosphorylates
CC       ZraR in response to high concentrations of zinc or lead in the medium
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- PTM: Autophosphorylated. {ECO:0000250}.
CC   -!- CAUTION: Was originally thought to be involved in the regulation of the
CC       labile hydrogenase activity. {ECO:0000305|PubMed:1756170}.
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DR   EMBL; AF170176; AAF33505.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL23001.1; -; Genomic_DNA.
DR   EMBL; M64988; AAA27148.1; -; mRNA.
DR   PIR; S19605; S19605.
DR   RefSeq; NP_463042.1; NC_003197.2.
DR   RefSeq; WP_001526924.1; NC_003197.2.
DR   AlphaFoldDB; P37461; -.
DR   SMR; P37461; -.
DR   STRING; 99287.STM4173; -.
DR   PaxDb; P37461; -.
DR   EnsemblBacteria; AAL23001; AAL23001; STM4173.
DR   GeneID; 1255699; -.
DR   KEGG; stm:STM4173; -.
DR   PATRIC; fig|99287.12.peg.4387; -.
DR   HOGENOM; CLU_000445_89_29_6; -.
DR   OMA; QAIFTPY; -.
DR   PhylomeDB; P37461; -.
DR   BioCyc; SENT99287:STM4173-MON; -.
DR   BRENDA; 2.7.13.3; 5542.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR029151; Sensor-like_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF103190; SSF103190; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix; Two-component regulatory system; Zinc.
FT   CHAIN           1..465
FT                   /note="Sensor protein ZraS"
FT                   /id="PRO_0000074913"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          253..461
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         256
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   465 AA;  51254 MW;  615F8346224FB3F6 CRC64;
     MSFIRLHKDA AATWLSRLLP AAIFILVGLF SIMVIRDYGR ESAAARQTLL EKGNVLIRAL
     ESGTRVGMGM RMHHAQQQTL LEEMAGQPGV LWFAVTDAQG VIITHSNPGM VGKSLYSPSE
     MHQLNPGPQE RWRRVDVAAN GETVPALEIY RQFQPLFGMR GHGMRGHGMA RSANDDEPAK
     QTIFIAFDAS ELAATQAREW RNTLIVLSAL AAVLLATLLA FFWHQRYQRS HRELLDAMKR
     KEKLVAMGHL AAGVAHEIRN PLSSIKGLAK YFAERTPAGG ESHELAQVMA KEADRLNRVV
     SELLELVKPA HLTLQTVNLN DIITHSLNLV SQDAQSREIQ LRFTANETLK RIQADPDRLT
     QVLLNLYLNA IHAIGRQGTI SVEAKESGTD RVIITVTDSG KGIAPDQLEA IFTPYFTTKA
     DGTGLGLAVV QNIIEQHGGA IKVKSIEGKG AVFTIWLPVI ARQQD