ZSA5A_HUMAN
ID ZSA5A_HUMAN Reviewed; 496 AA.
AC Q9BUG6; B4DX98; Q49A73; Q53F04; Q8N7B3;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 5A;
DE AltName: Full=Zinc finger protein 495;
GN Name=ZSCAN5A; Synonyms=ZNF495, ZSCAN5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-337.
RC TISSUE=Testis, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-159, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
RN [6]
RP VARIANT [LARGE SCALE ANALYSIS] GLN-146.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q9BUG6; Q96Q77: CIB3; NbExp=3; IntAct=EBI-8836980, EBI-10292696;
CC Q9BUG6; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-8836980, EBI-3867333;
CC Q9BUG6; Q9UMS0: NFU1; NbExp=3; IntAct=EBI-8836980, EBI-725252;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BUG6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BUG6-2; Sequence=VSP_055989, VSP_055990;
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DR EMBL; AK098700; BAC05386.1; -; mRNA.
DR EMBL; AK301873; BAG63310.1; -; mRNA.
DR EMBL; AK223485; BAD97205.1; -; mRNA.
DR EMBL; AC006116; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC011506; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC002636; AAH02636.1; -; mRNA.
DR EMBL; BC043232; AAH43232.1; -; mRNA.
DR CCDS; CCDS12941.1; -. [Q9BUG6-1]
DR RefSeq; NP_001308990.1; NM_001322061.1.
DR RefSeq; NP_001308991.1; NM_001322062.1.
DR RefSeq; NP_001308993.1; NM_001322064.1. [Q9BUG6-1]
DR RefSeq; NP_001308994.1; NM_001322065.1. [Q9BUG6-1]
DR RefSeq; NP_001308995.1; NM_001322066.1. [Q9BUG6-1]
DR RefSeq; NP_001308996.1; NM_001322067.1. [Q9BUG6-1]
DR RefSeq; NP_001308997.1; NM_001322068.1. [Q9BUG6-1]
DR RefSeq; NP_001308998.1; NM_001322069.1.
DR RefSeq; NP_001308999.1; NM_001322070.1. [Q9BUG6-1]
DR RefSeq; NP_001309001.1; NM_001322072.1. [Q9BUG6-1]
DR RefSeq; NP_001309002.1; NM_001322073.1.
DR RefSeq; NP_001309003.1; NM_001322074.1.
DR RefSeq; NP_001309004.1; NM_001322075.1.
DR RefSeq; NP_001309005.1; NM_001322076.1.
DR RefSeq; NP_001309006.1; NM_001322077.1. [Q9BUG6-2]
DR RefSeq; NP_001309007.1; NM_001322078.1.
DR RefSeq; NP_077279.1; NM_024303.2. [Q9BUG6-1]
DR RefSeq; XP_016882788.1; XM_017027299.1.
DR AlphaFoldDB; Q9BUG6; -.
DR SMR; Q9BUG6; -.
DR BioGRID; 122567; 37.
DR IntAct; Q9BUG6; 24.
DR MINT; Q9BUG6; -.
DR STRING; 9606.ENSP00000375593; -.
DR iPTMnet; Q9BUG6; -.
DR PhosphoSitePlus; Q9BUG6; -.
DR BioMuta; ZSCAN5A; -.
DR DMDM; 55976763; -.
DR jPOST; Q9BUG6; -.
DR MassIVE; Q9BUG6; -.
DR MaxQB; Q9BUG6; -.
DR PaxDb; Q9BUG6; -.
DR PeptideAtlas; Q9BUG6; -.
DR PRIDE; Q9BUG6; -.
DR ProteomicsDB; 79084; -. [Q9BUG6-1]
DR ABCD; Q9BUG6; 1 sequenced antibody.
DR Antibodypedia; 19613; 117 antibodies from 22 providers.
DR DNASU; 79149; -.
DR Ensembl; ENST00000391713.5; ENSP00000375593.1; ENSG00000131848.10. [Q9BUG6-1]
DR Ensembl; ENST00000587340.5; ENSP00000467631.1; ENSG00000131848.10. [Q9BUG6-1]
DR Ensembl; ENST00000683990.1; ENSP00000507065.1; ENSG00000131848.10. [Q9BUG6-1]
DR GeneID; 79149; -.
DR KEGG; hsa:79149; -.
DR MANE-Select; ENST00000683990.1; ENSP00000507065.1; NM_001322064.3; NP_001308993.1.
DR UCSC; uc002qmq.4; human. [Q9BUG6-1]
DR CTD; 79149; -.
DR DisGeNET; 79149; -.
DR GeneCards; ZSCAN5A; -.
DR HGNC; HGNC:23710; ZSCAN5A.
DR HPA; ENSG00000131848; Tissue enhanced (testis).
DR neXtProt; NX_Q9BUG6; -.
DR OpenTargets; ENSG00000131848; -.
DR PharmGKB; PA162411030; -.
DR VEuPathDB; HostDB:ENSG00000131848; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163230; -.
DR HOGENOM; CLU_002678_49_11_1; -.
DR InParanoid; Q9BUG6; -.
DR OMA; PTVCSIC; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9BUG6; -.
DR TreeFam; TF341155; -.
DR PathwayCommons; Q9BUG6; -.
DR SignaLink; Q9BUG6; -.
DR BioGRID-ORCS; 79149; 15 hits in 1100 CRISPR screens.
DR ChiTaRS; ZSCAN5A; human.
DR GenomeRNAi; 79149; -.
DR Pharos; Q9BUG6; Tdark.
DR PRO; PR:Q9BUG6; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9BUG6; protein.
DR Bgee; ENSG00000131848; Expressed in right testis and 106 other tissues.
DR ExpressionAtlas; Q9BUG6; baseline and differential.
DR Genevisible; Q9BUG6; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..496
FT /note="Zinc finger and SCAN domain-containing protein 5A"
FT /id="PRO_0000047752"
FT DOMAIN 44..126
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 356..378
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 384..406
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 412..434
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 440..462
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 468..490
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 152..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..233
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..263
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 159
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..11
FT /note="MAANCTSSWSL -> MIVRPPQPRET (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055989"
FT VAR_SEQ 12..128
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055990"
FT VARIANT 146
FT /note="E -> Q (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs145011101)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035603"
FT VARIANT 337
FT /note="G -> V (in dbSNP:rs34187696)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_042964"
FT CONFLICT 78
FT /note="H -> R (in Ref. 2; BAD97205)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="R -> G (in Ref. 2; BAD97205)"
FT /evidence="ECO:0000305"
FT CONFLICT 247..248
FT /note="TD -> N (in Ref. 1; BAC05386)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 496 AA; 55865 MW; DC8293A660C37329 CRC64;
MAANCTSSWS LGESCNRPGL ELPRSMASSE TQLGNHDVDP EISHVNFRMF SCPKESDPIQ
ALRKLTELCH LWLRPDLHTK EQILDMLVME QFMISMPQEL QVLVMMNGVQ SCKDLEDLLR
NNRRPKKWSV VTFHGKEYIV QDSDIEMAEA PSSVRDDLKD VSSQRASSVN QMRPGEGQAH
RELQILPRVP ALSRRQGEDF LLHKSIDVTG DPKSLRPKQT LEKDLKENRE ENPGLTSPEP
QLPKSPTDLV RAKEGKDPPK IASVENVDAD TPSACVVERE ASTHSGNRGD ALNLSSPKRS
KPDASSISQE EPQGEATPVG NRESPGQAGM NSIHSPGPAS PVSHPDGQEA KALPPFACDV
CEKRFTCNSK LVIHKRSHTG ERLFQCNLCG KRFMQLISLQ FHQRTHTGER PYTCDVCQKQ
FTQKSYLKCH KRSHTGEKPF ECKDCKKVFT YRGSLKEHQR IHSGEKPYKC SKCPRAFSRL
KLLRRHQKTH PEATSQ
