ZSC10_MOUSE
ID ZSC10_MOUSE Reviewed; 782 AA.
AC Q3URR7; B7ZP53; Q20D61; Q20D62; Q20D63;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 10;
DE AltName: Full=Zinc finger protein 206;
GN Name=Zscan10; Synonyms=Zfp206;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=129S1/Sv;
RX PubMed=16971461; DOI=10.1093/nar/gkl631;
RA Zhang W., Walker E., Tamplin O.J., Rossant J., Stanford W.L., Hughes T.R.;
RT "Zfp206 regulates ES cell gene expression and differentiation.";
RL Nucleic Acids Res. 34:4780-4790(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=129P2;
RX PubMed=17628018; DOI=10.1634/stemcells.2007-0085;
RA Wang Z.X., Kueh J.L., Teh C.H., Rossbach M., Lim L., Li P., Wong K.Y.,
RA Lufkin T., Robson P., Stanton L.W.;
RT "Zfp206 is a transcription factor that controls pluripotency of embryonic
RT stem cells.";
RL Stem Cells 25:2173-2182(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INDUCTION.
RX PubMed=17344211; DOI=10.1074/jbc.m611814200;
RA Wang Z.X., Teh C.H., Kueh J.L., Lufkin T., Robson P., Stanton L.W.;
RT "Oct4 and Sox2 directly regulate expression of another pluripotency
RT transcription factor, Zfp206, in embryonic stem cells.";
RL J. Biol. Chem. 282:12822-12830(2007).
RN [7]
RP FUNCTION, DNA-BINDING, AND INTERACTION WITH POU5F1 AND SOX2.
RX PubMed=19740739; DOI=10.1074/jbc.m109.016162;
RA Yu H.B., Kunarso G., Hong F.H., Stanton L.W.;
RT "Zfp206, Oct4, and Sox2 are integrated components of a transcriptional
RT regulatory network in embryonic stem cells.";
RL J. Biol. Chem. 284:31327-31335(2009).
CC -!- FUNCTION: Embryonic stem (ES) cell-specific transcription factor
CC required to maintain ES cell pluripotency. Can both activate and /or
CC repress expression of target genes, depending on the context.
CC Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence.
CC Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.
CC {ECO:0000269|PubMed:16971461, ECO:0000269|PubMed:17628018,
CC ECO:0000269|PubMed:19740739}.
CC -!- SUBUNIT: Interacts with POU5F1/OCT4 and SOX2.
CC {ECO:0000269|PubMed:19740739}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187,
CC ECO:0000269|PubMed:16971461, ECO:0000269|PubMed:17628018}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3URR7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3URR7-2; Sequence=VSP_039228;
CC Name=3;
CC IsoId=Q3URR7-3; Sequence=VSP_039227;
CC -!- TISSUE SPECIFICITY: Embryonic stem (ES) cell-specific. Not expressed in
CC adult, except in testis. {ECO:0000269|PubMed:16971461,
CC ECO:0000269|PubMed:17628018}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryogenesis.
CC {ECO:0000269|PubMed:16971461}.
CC -!- INDUCTION: Transcriptionally regulated by POU5F1/OCT4 and SOX2.
CC {ECO:0000269|PubMed:17344211}.
CC -!- PTM: Methylated at Gln-485 by N6AMT1. {ECO:0000250|UniProtKB:Q96SZ4}.
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DR EMBL; DQ323929; ABC54589.1; -; mRNA.
DR EMBL; DQ323930; ABC54590.1; -; mRNA.
DR EMBL; DQ323931; ABC54591.1; -; mRNA.
DR EMBL; EF152498; ABM45916.1; -; mRNA.
DR EMBL; AK141259; BAE24621.1; -; mRNA.
DR EMBL; CH466606; EDL22251.1; -; Genomic_DNA.
DR EMBL; BC145638; AAI45639.1; -; mRNA.
DR CCDS; CCDS28451.1; -. [Q3URR7-1]
DR CCDS; CCDS70766.1; -. [Q3URR7-2]
DR CCDS; CCDS79510.1; -. [Q3URR7-3]
DR RefSeq; NP_001028597.2; NM_001033425.4. [Q3URR7-1]
DR RefSeq; NP_001276410.1; NM_001289481.1. [Q3URR7-2]
DR RefSeq; NP_001276411.1; NM_001289482.1. [Q3URR7-3]
DR RefSeq; NP_001276412.1; NM_001289483.1.
DR RefSeq; NP_001276413.1; NM_001289484.1.
DR PDB; 4E6S; X-ray; 1.85 A; A=36-128.
DR PDBsum; 4E6S; -.
DR AlphaFoldDB; Q3URR7; -.
DR SMR; Q3URR7; -.
DR BioGRID; 237126; 24.
DR STRING; 10090.ENSMUSP00000093255; -.
DR iPTMnet; Q3URR7; -.
DR PhosphoSitePlus; Q3URR7; -.
DR PaxDb; Q3URR7; -.
DR PeptideAtlas; Q3URR7; -.
DR PRIDE; Q3URR7; -.
DR ProteomicsDB; 302148; -. [Q3URR7-1]
DR ProteomicsDB; 302149; -. [Q3URR7-2]
DR ProteomicsDB; 302150; -. [Q3URR7-3]
DR Antibodypedia; 58023; 28 antibodies from 11 providers.
DR DNASU; 332221; -.
DR Ensembl; ENSMUST00000095595; ENSMUSP00000093255; ENSMUSG00000023902. [Q3URR7-1]
DR Ensembl; ENSMUST00000115509; ENSMUSP00000111171; ENSMUSG00000023902. [Q3URR7-3]
DR Ensembl; ENSMUST00000120967; ENSMUSP00000113386; ENSMUSG00000023902. [Q3URR7-2]
DR GeneID; 332221; -.
DR KEGG; mmu:332221; -.
DR UCSC; uc008ask.3; mouse. [Q3URR7-2]
DR UCSC; uc008asl.2; mouse. [Q3URR7-1]
DR UCSC; uc008asm.2; mouse. [Q3URR7-3]
DR CTD; 84891; -.
DR MGI; MGI:3040700; Zscan10.
DR VEuPathDB; HostDB:ENSMUSG00000023902; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162513; -.
DR HOGENOM; CLU_002678_49_8_1; -.
DR InParanoid; Q3URR7; -.
DR OMA; THQLRSH; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q3URR7; -.
DR TreeFam; TF338010; -.
DR BioGRID-ORCS; 332221; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q3URR7; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q3URR7; protein.
DR Bgee; ENSMUSG00000023902; Expressed in epiblast (generic) and 63 other tissues.
DR ExpressionAtlas; Q3URR7; baseline and differential.
DR Genevisible; Q3URR7; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048863; P:stem cell differentiation; TAS:UniProtKB.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 12.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 14.
DR SUPFAM; SSF57667; SSF57667; 8.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 14.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 14.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; DNA-binding; Metal-binding;
KW Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..782
FT /note="Zinc finger and SCAN domain-containing protein 10"
FT /id="PRO_0000394248"
FT DOMAIN 1..71
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 292..315
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 321..343
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 349..371
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 377..399
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 421..443
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 467..489
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 495..517
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 523..545
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 551..573
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 579..601
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 607..629
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 635..657
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 669..691
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 697..719
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..522
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..230
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 160
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SZ4"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SZ4"
FT MOD_RES 485
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250|UniProtKB:Q96SZ4"
FT VAR_SEQ 131..240
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16971461"
FT /id="VSP_039227"
FT VAR_SEQ 328..359
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16971461"
FT /id="VSP_039228"
FT CONFLICT 306
FT /note="V -> E (in Ref. 1; ABC54589/ABC54590/ABC54591, 2;
FT ABM45916 and 4; EDL22251)"
FT /evidence="ECO:0000305"
FT CONFLICT 546
FT /note="V -> L (in Ref. 3; BAE24621)"
FT /evidence="ECO:0000305"
FT HELIX 39..47
FT /evidence="ECO:0007829|PDB:4E6S"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:4E6S"
FT HELIX 57..72
FT /evidence="ECO:0007829|PDB:4E6S"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:4E6S"
FT HELIX 79..93
FT /evidence="ECO:0007829|PDB:4E6S"
FT HELIX 97..100
FT /evidence="ECO:0007829|PDB:4E6S"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:4E6S"
FT HELIX 111..116
FT /evidence="ECO:0007829|PDB:4E6S"
SQ SEQUENCE 782 AA; 88355 MW; B521507A5C617AED CRC64;
MLAEPVPDAL EQEHPGAVKL EEDEVGEEDP RLAESRPRPE VAHQLFRCFQ YQEDMGPRAS
LGRLRELCNH WLRPALHTKK QILELLVLEQ FLSVLPPHVL SRLHGQPLRD GEEVVQLLEG
VPRDISHMGP LDFSFSAGKN APADIISEEQ NSPSQVPSHS PQTELPSEEI PALHPLNELP
PPQPAPIRPA EPEEWRLAPS SNWPMSPEPQ EILQDPRESN PSQGPSWLEE NSRDQELAAV
LESLTFEDTS EKRAWPANPL GFGSRMPDNE ELKVEEPKVT TWPVVIGAES QTEKPEVAGE
PLTQTVGQET SSTGWGGTPA DGSEVVKVRG ASDAPEPQGE MQFICTYCGV NFPEMSHLQA
HQLQSHPNLQ PHPSSRSFRC LWCGKTFGRS SILKLHMRTH TDERPHACHL CNRRFRQSSH
LTKHLLTHSS EPAFRCAECN QGFQRRSSLM QHLLAHAQGK NLTPNPEGKT KVPEMAAVLC
SHCGQTFKRR SSLKRHLRNH AKDKDHLSSE DPGSLSSSQE SNPYVCSDCG KAFRQSEQLM
IHTRRVHTRE RPFSCQVCGR CFTQNSQLIS HQQIHTGEKP HACPQCSKRF VRRAGLARHL
LTHGSLRPYH CAQCGKSFRQ MRDLTRHVRC HTGEKPCRCN ECGEGFTQNA HLARHQRIHT
GEKPHACDIC GHRFRNSSNL ARHRRSHTGE RPYSCPTCGR SFRRNAHLQR HLITHTGSKQ
EKEVPQECPE CGKSFNRSCN LLRHLLVHTG ARPYSCALCG RSFSRNSHLL RHLRTHARES
LY
