ZSC12_HUMAN
ID ZSC12_HUMAN Reviewed; 611 AA.
AC O43309; A0A024RCI2; A8K187; O43724;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 12;
DE AltName: Full=Zinc finger protein 305;
DE AltName: Full=Zinc finger protein 96;
GN Name=ZSCAN12; Synonyms=KIAA0426, ZNF305, ZNF96;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=9455477; DOI=10.1093/dnares/4.5.307;
RA Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. VIII. 78
RT new cDNA clones from brain which code for large proteins in vitro.";
RL DNA Res. 4:307-313(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-20; LYS-25 AND LYS-196, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC O43309; Q8TD16-2: BICD2; NbExp=3; IntAct=EBI-1210440, EBI-11975051;
CC O43309; Q9H3R5: CENPH; NbExp=3; IntAct=EBI-1210440, EBI-1003700;
CC O43309; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-1210440, EBI-739624;
CC O43309; Q3B820: FAM161A; NbExp=3; IntAct=EBI-1210440, EBI-719941;
CC O43309; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-1210440, EBI-10171774;
CC O43309; P02545: LMNA; NbExp=3; IntAct=EBI-1210440, EBI-351935;
CC O43309; Q9UJV3-2: MID2; NbExp=6; IntAct=EBI-1210440, EBI-10172526;
CC O43309; Q5JR59: MTUS2; NbExp=3; IntAct=EBI-1210440, EBI-742948;
CC O43309; Q96JS3: PGBD1; NbExp=3; IntAct=EBI-1210440, EBI-10290053;
CC O43309; P63244: RACK1; NbExp=3; IntAct=EBI-1210440, EBI-296739;
CC O43309; P57086: SCAND1; NbExp=5; IntAct=EBI-1210440, EBI-745846;
CC O43309; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-1210440, EBI-5235340;
CC O43309; P78362: SRPK2; NbExp=3; IntAct=EBI-1210440, EBI-593303;
CC O43309; Q9Y2D8: SSX2IP; NbExp=3; IntAct=EBI-1210440, EBI-2212028;
CC O43309; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-1210440, EBI-1105213;
CC O43309; P98155-2: VLDLR; NbExp=3; IntAct=EBI-1210440, EBI-12047495;
CC O43309; O75436: VPS26A; NbExp=3; IntAct=EBI-1210440, EBI-1043891;
CC O43309; Q9P0L1: ZKSCAN7; NbExp=3; IntAct=EBI-1210440, EBI-743851;
CC O43309; P17028: ZNF24; NbExp=3; IntAct=EBI-1210440, EBI-707773;
CC O43309; Q8NF99-2: ZNF397; NbExp=3; IntAct=EBI-1210440, EBI-11524467;
CC O43309; Q9NWS9-2: ZNF446; NbExp=7; IntAct=EBI-1210440, EBI-740232;
CC O43309; Q8WTR7: ZNF473; NbExp=3; IntAct=EBI-1210440, EBI-751409;
CC O43309; Q96IT1: ZNF496; NbExp=3; IntAct=EBI-1210440, EBI-743906;
CC O43309; Q9Y5A6: ZSCAN21; NbExp=3; IntAct=EBI-1210440, EBI-10281938;
CC O43309; Q9NX65: ZSCAN32; NbExp=5; IntAct=EBI-1210440, EBI-739949;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O43309-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O43309-2; Sequence=VSP_061227;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA24856.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB007886; BAA24856.2; ALT_INIT; mRNA.
DR EMBL; AK289802; BAF82491.1; -; mRNA.
DR EMBL; Z98745; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX03161.1; -; Genomic_DNA.
DR EMBL; CH471081; EAX03162.1; -; Genomic_DNA.
DR EMBL; BC041661; AAH41661.1; -; mRNA.
DR AlphaFoldDB; O43309; -.
DR SMR; O43309; -.
DR IntAct; O43309; 54.
DR STRING; 9606.ENSP00000354305; -.
DR iPTMnet; O43309; -.
DR PhosphoSitePlus; O43309; -.
DR BioMuta; ZSCAN12; -.
DR EPD; O43309; -.
DR jPOST; O43309; -.
DR MassIVE; O43309; -.
DR MaxQB; O43309; -.
DR PaxDb; O43309; -.
DR PeptideAtlas; O43309; -.
DR PRIDE; O43309; -.
DR ABCD; O43309; 2 sequenced antibodies.
DR Antibodypedia; 1809; 105 antibodies from 17 providers.
DR Ensembl; ENST00000361028.5; ENSP00000354305.1; ENSG00000158691.15. [O43309-2]
DR Ensembl; ENST00000396827.3; ENSP00000380039.3; ENSG00000158691.15. [O43309-2]
DR Ensembl; ENST00000684592.1; ENSP00000507347.1; ENSG00000158691.15. [O43309-1]
DR GeneID; 9753; -.
DR MANE-Select; ENST00000684592.1; ENSP00000507347.1; NM_001163391.2; NP_001156863.1.
DR UCSC; uc063mll.1; human. [O43309-1]
DR CTD; 9753; -.
DR GeneCards; ZSCAN12; -.
DR HGNC; HGNC:13172; ZSCAN12.
DR HPA; ENSG00000158691; Low tissue specificity.
DR MIM; 603978; gene.
DR neXtProt; NX_O43309; -.
DR OpenTargets; ENSG00000158691; -.
DR PharmGKB; PA37744; -.
DR VEuPathDB; HostDB:ENSG00000158691; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163105; -.
DR HOGENOM; CLU_002678_57_1_1; -.
DR InParanoid; O43309; -.
DR OMA; QCEPQAG; -.
DR PhylomeDB; O43309; -.
DR TreeFam; TF338146; -.
DR PathwayCommons; O43309; -.
DR SignaLink; O43309; -.
DR Pharos; O43309; Tdark.
DR PRO; PR:O43309; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; O43309; protein.
DR Bgee; ENSG00000158691; Expressed in ventricular zone and 144 other tissues.
DR ExpressionAtlas; O43309; baseline and differential.
DR Genevisible; O43309; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 12.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 12.
DR SUPFAM; SSF57667; SSF57667; 7.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 11.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 12.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..611
FT /note="Zinc finger and SCAN domain-containing protein 12"
FT /id="PRO_0000047403"
FT DOMAIN 46..128
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 274..296
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 302..324
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 330..352
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 358..380
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 386..408
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 414..436
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 442..463
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 469..491
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 497..519
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 525..547
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 553..575
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 581..603
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 154..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..248
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 20
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 25
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 196
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 566..611
FT /note="SDLSKHQRIHNRRGTYVCKECGKSFRQNSALTQHQTIHKGEKSVSV -> KK
FT TSYKEILLKNHSEPQAGVNLLLSSLIPEWQSCFRRDL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_061227"
FT CONFLICT 392
FT /note="N -> D (in Ref. 2; BAF82491)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 611 AA; 70960 MW; 046FFA288FAB94CE CRC64;
MASTWAIQAH MDQDEPLEVK IEEEKYTTRQ DWDLRKNNTH SREVFRQYFR QFCYQETSGP
REALSRLREL CHQWLRPETH TKEQILELLV LEQFLTILPE ELQAWVQEQH PESGEEVVTV
LEDLERELDE PGEQVSVHTG EQEMFLQETV RLRKEGEPSM SLQSMKAQPK YESPELESQQ
EQVLDVETGN EYGNLKQEVS EEMEPHGKTS SKFENDMSKS ARCGETREPE EITEEPSACS
REDKQPTCDE NGVSLTENSD HTEHQRICPG EESYGCDDCG KAFSQHSHLI EHQRIHTGDR
PYKCEECGKA FRGRTVLIRH KIIHTGEKPY KCNECGKAFG RWSALNQHQR LHTGEKHYHC
NDCGKAFSQK AGLFHHIKIH TRDKPYQCTQ CNKSFSRRSI LTQHQGVHTG AKPYECNECG
KAFVYNSSLV SHQEIHHKEK CYQCKECGKS FSQSGLIQHQ RIHTGEKPYK CDVCEKAFIQ
RTSLTEHQRI HTGERPYKCD KCGKAFTQRS VLTEHQRIHT GERPYKCDEC GNAFRGITSL
IQHQRIHTGE KPYQCDECGK AFRQRSDLSK HQRIHNRRGT YVCKECGKSF RQNSALTQHQ
TIHKGEKSVS V
