ZSC20_MOUSE
ID ZSC20_MOUSE Reviewed; 1030 AA.
AC B2KFW1; B1AS93; Q3UNF0; Q6DFW6; Q8BJ07;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 20;
DE AltName: Full=Zinc finger protein 31;
GN Name=Zscan20; Synonyms=Zfp31;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Head, Lymph node, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B2KFW1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B2KFW1-2; Sequence=VSP_036740;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH76602.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE25797.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK047820; BAC33164.1; -; mRNA.
DR EMBL; AK144249; BAE25797.1; ALT_INIT; mRNA.
DR EMBL; AK153688; BAE32146.1; -; mRNA.
DR EMBL; AK153845; BAE32209.1; -; mRNA.
DR EMBL; AL611969; CAM23047.1; -; Genomic_DNA.
DR EMBL; CU210837; CAQ51752.1; -; Genomic_DNA.
DR EMBL; BC065079; AAH65079.1; -; mRNA.
DR EMBL; BC076602; AAH76602.1; ALT_INIT; mRNA.
DR CCDS; CCDS18672.2; -. [B2KFW1-1]
DR RefSeq; NP_808426.2; NM_177758.4. [B2KFW1-1]
DR RefSeq; XP_006503194.1; XM_006503131.2. [B2KFW1-1]
DR RefSeq; XP_006503196.1; XM_006503133.2. [B2KFW1-1]
DR RefSeq; XP_006503197.1; XM_006503134.2. [B2KFW1-1]
DR RefSeq; XP_011238844.1; XM_011240542.2. [B2KFW1-1]
DR RefSeq; XP_011238845.1; XM_011240543.2. [B2KFW1-1]
DR RefSeq; XP_011238849.1; XM_011240547.2. [B2KFW1-2]
DR AlphaFoldDB; B2KFW1; -.
DR SMR; B2KFW1; -.
DR STRING; 10090.ENSMUSP00000095487; -.
DR iPTMnet; B2KFW1; -.
DR PhosphoSitePlus; B2KFW1; -.
DR PaxDb; B2KFW1; -.
DR PeptideAtlas; B2KFW1; -.
DR PRIDE; B2KFW1; -.
DR ProteomicsDB; 275244; -. [B2KFW1-1]
DR ProteomicsDB; 275245; -. [B2KFW1-2]
DR Antibodypedia; 8548; 148 antibodies from 27 providers.
DR DNASU; 269585; -.
DR Ensembl; ENSMUST00000097877; ENSMUSP00000095487; ENSMUSG00000061894. [B2KFW1-1]
DR GeneID; 269585; -.
DR KEGG; mmu:269585; -.
DR UCSC; uc008uvd.2; mouse. [B2KFW1-1]
DR CTD; 7579; -.
DR MGI; MGI:2679268; Zscan20.
DR VEuPathDB; HostDB:ENSMUSG00000061894; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000161580; -.
DR HOGENOM; CLU_002678_88_0_1; -.
DR InParanoid; B2KFW1; -.
DR OMA; EQEQWDV; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; B2KFW1; -.
DR TreeFam; TF337082; -.
DR BioGRID-ORCS; 269585; 5 hits in 71 CRISPR screens.
DR PRO; PR:B2KFW1; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; B2KFW1; protein.
DR Bgee; ENSMUSG00000061894; Expressed in ureteric bud trunk and 99 other tissues.
DR ExpressionAtlas; B2KFW1; baseline and differential.
DR Genevisible; B2KFW1; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR044822; Myb_DNA-bind_4.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF13837; Myb_DNA-bind_4; 2.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 8.
DR SMART; SM00717; SANT; 2.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 9.
DR SUPFAM; SSF57667; SSF57667; 6.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 10.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1030
FT /note="Zinc finger and SCAN domain-containing protein 20"
FT /id="PRO_0000367588"
FT DOMAIN 45..127
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 697..719
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 725..747
FT /note="C2H2-type 2; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 753..775
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 781..803
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 862..884
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 890..912
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 918..940
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 946..968
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 974..996
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 1002..1024
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 22..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 178..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 411..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 578..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 801..820
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 828..850
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..429
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_036740"
FT CONFLICT 176
FT /note="R -> C (in Ref. 1; CAQ51752)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="R -> S (in Ref. 1; BAE25797)"
FT /evidence="ECO:0000305"
FT CONFLICT 627
FT /note="D -> G (in Ref. 1; BAE25797)"
FT /evidence="ECO:0000305"
FT CONFLICT 857
FT /note="S -> P (in Ref. 1; BAE25797)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1030 AA; 115379 MW; 287C0AA0ECE6BE07 CRC64;
MMAVASPPPE PEDLLIVKLE EDSWGSDSRP EKESHSPVPG PEVSRRCFRQ FRYRDAAGPH
EAFSQLWALC CRWLRPELRL KEQILELLVL EQFLSILPRE VQTWVQARHP ESGEEAVALV
EDWHREAWAA GQQGLELCSE DSRSFEAVQE FQRFQLQPVT HGSEGQPRKQ WVENARPDLS
KMPPESLKES AVLTPQAPTV PKMASIGDWE VAGKSQETPS PSRQAKKEPC QDPAGGDRGD
SACLGVPASK PSATSQQEQG PEIWGLSLIN SGNGSAADDS LDSAQDKPVQ AVAQADSRAW
GEPCQWGAED MKVSGVHWGY EETKTFLAIL SESPFSEKLQ TCHQNRQVYR AIAERLRARG
FLRTLEQCRY RVKNLLRNYR KAKNSHPPGT CPFYEELEAL VRARTAIRRT SGGPGEAVAL
PRLGDSDTEM DDQDEGSWEP EETVEDCSGS GLAAEESLQG PRIAGGPALL QSRIAGVHWG
FEETKVFLAI LSESPFAEKL RTCHQNSQIY RAIAERLRAL GFLRTLEQCR YRFKNLLRSY
RKAKSSCPPG TCPFYEEMDS LMRARTVIRA VEMVGEATGL PGSGQSSTEA DDQEAWGEME
DEDAVRLLTP DSQPADAGFE LKREEEDQIS EQDVLGDLPG ALSRYTTKAV CQPCDWGEDH
VNGNEGEWRN TWEECSSEED LEKLIDHQGL YLTEKPYGCD TRAKSFSRKV HFFAPQRTHS
SEKPYKCLGS GKSFSDRANL STHQRIHIGE KPYRCLECGK SFNDPSNLIT HQRTHTGEKP
YKCGLCWKSF NQSSNLLKHQ RVHLGGPPNQ RDEPGENFGQ SLSYSAHWRR NSTQEGPKEP
QNISMGADSP GACHPNSGEK LYSCPECGRC FSKSSALTSH QRIHSGEKPY ECAVCGKSFS
KSSSLANHRR THTGEKPHKC ADCGKCFSER SKLITHQRVH TGEKPYECPE CGKFFRDRSN
LITHQRIHTG EKPYKCRECG KCFNQSSSLI IHQRIHTGEK PYKCTECGKD FNNSSHFSAH
RRTHAGGKAL
