ZSC25_HUMAN
ID ZSC25_HUMAN Reviewed; 544 AA.
AC Q6NSZ9; A4D290; D6W5T5; Q14C82; Q14C99; Q5EBM9; Q6DJZ0; Q6N032; Q6ZML3;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 3.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 25;
DE AltName: Full=Zinc finger protein 498;
GN Name=ZSCAN25; Synonyms=ZNF498;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Spleen;
RA Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.;
RT "The nucleotide sequence of a long cDNA clone isolated from human spleen.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Fetal brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-544 (ISOFORM 1), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 7-544 (ISOFORMS 2 AND 4).
RC TISSUE=Lung, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-128, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25755297; DOI=10.1074/mcp.o114.044792;
RA Xiao Z., Chang J.G., Hendriks I.A., Sigurdsson J.O., Olsen J.V.,
RA Vertegaal A.C.;
RT "System-wide analysis of SUMOylation dynamics in response to replication
RT stress reveals novel small ubiquitin-like modified target proteins and
RT acceptor lysines relevant for genome stability.";
RL Mol. Cell. Proteomics 14:1419-1434(2015).
RN [8]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-3; LYS-22; LYS-128; LYS-278 AND
RP LYS-285, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation. {ECO:0000250}.
CC -!- INTERACTION:
CC Q6NSZ9-2; Q9BWT7: CARD10; NbExp=3; IntAct=EBI-14650477, EBI-3866279;
CC Q6NSZ9-2; P68400: CSNK2A1; NbExp=3; IntAct=EBI-14650477, EBI-347804;
CC Q6NSZ9-2; Q92997: DVL3; NbExp=3; IntAct=EBI-14650477, EBI-739789;
CC Q6NSZ9-2; P57086: SCAND1; NbExp=3; IntAct=EBI-14650477, EBI-745846;
CC Q6NSZ9-2; P10073: ZSCAN22; NbExp=3; IntAct=EBI-14650477, EBI-10178224;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q6NSZ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NSZ9-2; Sequence=VSP_029147;
CC Name=3;
CC IsoId=Q6NSZ9-3; Sequence=VSP_029145;
CC Name=4;
CC IsoId=Q6NSZ9-4; Sequence=VSP_029146;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI14574.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAI14942.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
CC Sequence=BAD18712.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAL23869.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK160369; BAD18712.1; ALT_INIT; mRNA.
DR EMBL; BX640720; CAE45839.1; -; mRNA.
DR EMBL; CH236956; EAL23869.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH471091; EAW76643.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76646.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76647.1; -; Genomic_DNA.
DR EMBL; BC069644; AAH69644.2; -; mRNA.
DR EMBL; BC074902; AAH74902.3; -; mRNA.
DR EMBL; BC074903; AAH74903.3; -; mRNA.
DR EMBL; BC089402; AAH89402.1; -; mRNA.
DR EMBL; BC114573; AAI14574.1; ALT_INIT; mRNA.
DR EMBL; BC114941; AAI14942.1; ALT_SEQ; mRNA.
DR CCDS; CCDS5671.2; -. [Q6NSZ9-1]
DR RefSeq; NP_660090.2; NM_145115.2. [Q6NSZ9-1]
DR RefSeq; XP_005250251.1; XM_005250194.2.
DR RefSeq; XP_011514207.1; XM_011515905.2. [Q6NSZ9-1]
DR RefSeq; XP_011514208.1; XM_011515906.2.
DR RefSeq; XP_011514209.1; XM_011515907.2. [Q6NSZ9-1]
DR RefSeq; XP_011514210.1; XM_011515908.2.
DR RefSeq; XP_016867313.1; XM_017011824.1. [Q6NSZ9-1]
DR RefSeq; XP_016867314.1; XM_017011825.1.
DR RefSeq; XP_016867315.1; XM_017011826.1.
DR AlphaFoldDB; Q6NSZ9; -.
DR SMR; Q6NSZ9; -.
DR BioGRID; 128753; 73.
DR IntAct; Q6NSZ9; 29.
DR STRING; 9606.ENSP00000377708; -.
DR iPTMnet; Q6NSZ9; -.
DR PhosphoSitePlus; Q6NSZ9; -.
DR BioMuta; ZSCAN25; -.
DR DMDM; 160359044; -.
DR EPD; Q6NSZ9; -.
DR jPOST; Q6NSZ9; -.
DR MassIVE; Q6NSZ9; -.
DR MaxQB; Q6NSZ9; -.
DR PaxDb; Q6NSZ9; -.
DR PeptideAtlas; Q6NSZ9; -.
DR PRIDE; Q6NSZ9; -.
DR ProteomicsDB; 66645; -. [Q6NSZ9-1]
DR ProteomicsDB; 66646; -. [Q6NSZ9-2]
DR ProteomicsDB; 66647; -. [Q6NSZ9-3]
DR ProteomicsDB; 66648; -. [Q6NSZ9-4]
DR ABCD; Q6NSZ9; 2 sequenced antibodies.
DR Antibodypedia; 30418; 197 antibodies from 25 providers.
DR DNASU; 221785; -.
DR Ensembl; ENST00000334715.7; ENSP00000334800.3; ENSG00000197037.11. [Q6NSZ9-1]
DR Ensembl; ENST00000394150.5; ENSP00000377706.1; ENSG00000197037.11. [Q6NSZ9-4]
DR Ensembl; ENST00000394152.7; ENSP00000377708.2; ENSG00000197037.11. [Q6NSZ9-1]
DR GeneID; 221785; -.
DR KEGG; hsa:221785; -.
DR MANE-Select; ENST00000394152.7; ENSP00000377708.2; NM_145115.3; NP_660090.2.
DR UCSC; uc003url.1; human. [Q6NSZ9-1]
DR CTD; 221785; -.
DR DisGeNET; 221785; -.
DR GeneCards; ZSCAN25; -.
DR HGNC; HGNC:21961; ZSCAN25.
DR HPA; ENSG00000197037; Low tissue specificity.
DR neXtProt; NX_Q6NSZ9; -.
DR OpenTargets; ENSG00000197037; -.
DR PharmGKB; PA134962723; -.
DR VEuPathDB; HostDB:ENSG00000197037; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162113; -.
DR HOGENOM; CLU_002678_53_4_1; -.
DR InParanoid; Q6NSZ9; -.
DR OMA; VNTRDQE; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q6NSZ9; -.
DR TreeFam; TF350842; -.
DR PathwayCommons; Q6NSZ9; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q6NSZ9; -.
DR BioGRID-ORCS; 221785; 14 hits in 1093 CRISPR screens.
DR ChiTaRS; ZSCAN25; human.
DR GenomeRNAi; 221785; -.
DR Pharos; Q6NSZ9; Tdark.
DR PRO; PR:Q6NSZ9; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q6NSZ9; protein.
DR Bgee; ENSG00000197037; Expressed in tibialis anterior and 158 other tissues.
DR ExpressionAtlas; Q6NSZ9; baseline and differential.
DR Genevisible; Q6NSZ9; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 7.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..544
FT /note="Zinc finger and SCAN domain-containing protein 25"
FT /id="PRO_0000309332"
FT DOMAIN 42..124
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 348..370
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 375..397
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 403..425
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 431..453
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 459..480
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 486..508
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 514..536
FT /note="C2H2-type 7; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 157..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 3
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 22
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 128
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25755297,
FT ECO:0007744|PubMed:28112733"
FT CROSSLNK 278
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 285
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..216
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029145"
FT VAR_SEQ 130..544
FT /note="VPCHRQGEQEETALCRGAWEPGIQLGPVEVKPEWGMPPGEGVQGPDPGTEEQ
FT LSQDPGDETRAFQEQALPVLQAGPGLPAVNPRDQEMAAGFFTAGSQGLGPFKDMALAFP
FT EEEWRHVTPAQIDCFGEYVEPQDCRVSPGGGSKEKEAKPPQEDLKGALVALTSERFGEA
FT SLQGPGLGRVCEQEPGGPAGSAPGLPPPQHGAIPLPDEVKTHSSFWKPFQCPECGKGFS
FT RSSNLVRHQRTHEEKSYGCVECGKGFTLREYLMKHQRTHLGKRPYVCSECWKTFSQRHH
FT LEVHQRSHTGEKPYKCGDCWKSFSRRQHLQVHRRTHTGEKPYTCECGKSFSRNANLAVH
FT RRAHTGEKPYGCQVCGKRFSKGERLVRHQRIHTGEKPYHCPACGRSFNQRSILNRHQKT
FT QHRQEPLVQ -> VGSMPQAGRAGGNSTLQRRLGARHPAGASGGQA (in isoform
FT 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:17974005"
FT /id="VSP_029146"
FT VAR_SEQ 197..268
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029147"
FT VARIANT 325
FT /note="P -> A (in dbSNP:rs10239632)"
FT /id="VAR_036931"
SQ SEQUENCE 544 AA; 61474 MW; E63AA7ACDA7E7E8B CRC64;
MLKEHPEMAE APQQQLGIPV VKLEKELPWG RGREDPSPET FRLRFRQFRY QEAAGPQEAL
RELQELCRRW LRPELHTKEQ ILELLVLEQF LTILPREFYA WIREHGPESG KALAAMVEDL
TERALEAKAV PCHRQGEQEE TALCRGAWEP GIQLGPVEVK PEWGMPPGEG VQGPDPGTEE
QLSQDPGDET RAFQEQALPV LQAGPGLPAV NPRDQEMAAG FFTAGSQGLG PFKDMALAFP
EEEWRHVTPA QIDCFGEYVE PQDCRVSPGG GSKEKEAKPP QEDLKGALVA LTSERFGEAS
LQGPGLGRVC EQEPGGPAGS APGLPPPQHG AIPLPDEVKT HSSFWKPFQC PECGKGFSRS
SNLVRHQRTH EEKSYGCVEC GKGFTLREYL MKHQRTHLGK RPYVCSECWK TFSQRHHLEV
HQRSHTGEKP YKCGDCWKSF SRRQHLQVHR RTHTGEKPYT CECGKSFSRN ANLAVHRRAH
TGEKPYGCQV CGKRFSKGER LVRHQRIHTG EKPYHCPACG RSFNQRSILN RHQKTQHRQE
PLVQ
