ZSC30_HUMAN
ID ZSC30_HUMAN Reviewed; 494 AA.
AC Q86W11; B4E0N0; Q6ZNB3; Q96PN3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 30;
DE AltName: Full=ZNF-WYM;
DE AltName: Full=Zinc finger protein 397 opposite strand;
DE AltName: Full=Zinc finger protein 397OS;
GN Name=ZSCAN30; Synonyms=ZNF397OS;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12801647; DOI=10.1016/s0378-1119(03)00551-1;
RA Wu Y., Yu L., Bi G., Luo K., Zhou G., Zhao S.;
RT "Identification and characterization of two novel human SCAN domain-
RT containing zinc finger genes ZNF396 and ZNF397.";
RL Gene 310:193-201(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Chondrocyte, and Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 185-494 (ISOFORM 1).
RA Lu X.-W., Cui L.-X., Li Y.-H.;
RT "Activated B lymphocyte gene.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-197, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q86W11; O43559: FRS3; NbExp=3; IntAct=EBI-11793064, EBI-725515;
CC Q86W11; Q15287: RNPS1; NbExp=3; IntAct=EBI-11793064, EBI-395959;
CC Q86W11; Q969J2: ZKSCAN4; NbExp=3; IntAct=EBI-11793064, EBI-2818641;
CC Q86W11; Q9NWS9-2: ZNF446; NbExp=3; IntAct=EBI-11793064, EBI-740232;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86W11-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86W11-3; Sequence=VSP_027861, VSP_027862;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL26786.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AY234408; AAO89074.1; -; mRNA.
DR EMBL; AK131291; BAD18462.1; -; mRNA.
DR EMBL; AK303447; BAG64492.1; -; mRNA.
DR EMBL; CH471088; EAX01344.1; -; Genomic_DNA.
DR EMBL; AF303374; AAL26786.1; ALT_SEQ; mRNA.
DR CCDS; CCDS42427.1; -. [Q86W11-1]
DR RefSeq; NP_001106205.1; NM_001112734.3. [Q86W11-1]
DR RefSeq; NP_001159484.1; NM_001166012.2. [Q86W11-1]
DR RefSeq; NP_001275640.1; NM_001288711.1.
DR RefSeq; XP_005258240.1; XM_005258183.4. [Q86W11-1]
DR RefSeq; XP_006722434.1; XM_006722371.3. [Q86W11-1]
DR RefSeq; XP_011524091.1; XM_011525789.2.
DR RefSeq; XP_016881004.1; XM_017025515.1. [Q86W11-1]
DR RefSeq; XP_016881008.1; XM_017025519.1.
DR RefSeq; XP_016881009.1; XM_017025520.1.
DR RefSeq; XP_016881010.1; XM_017025521.1.
DR RefSeq; XP_016881011.1; XM_017025522.1.
DR AlphaFoldDB; Q86W11; -.
DR SMR; Q86W11; -.
DR BioGRID; 755850; 15.
DR IntAct; Q86W11; 12.
DR MINT; Q86W11; -.
DR STRING; 9606.ENSP00000392371; -.
DR iPTMnet; Q86W11; -.
DR PhosphoSitePlus; Q86W11; -.
DR BioMuta; ZSCAN30; -.
DR DMDM; 74759452; -.
DR EPD; Q86W11; -.
DR jPOST; Q86W11; -.
DR MassIVE; Q86W11; -.
DR MaxQB; Q86W11; -.
DR PaxDb; Q86W11; -.
DR PeptideAtlas; Q86W11; -.
DR PRIDE; Q86W11; -.
DR ProteomicsDB; 70101; -. [Q86W11-1]
DR ProteomicsDB; 70102; -. [Q86W11-3]
DR ABCD; Q86W11; 2 sequenced antibodies.
DR Antibodypedia; 1836; 10 antibodies from 6 providers.
DR DNASU; 100101467; -.
DR Ensembl; ENST00000333206.10; ENSP00000329738.4; ENSG00000186814.14. [Q86W11-1]
DR Ensembl; ENST00000420878.7; ENSP00000392371.2; ENSG00000186814.14. [Q86W11-1]
DR GeneID; 100101467; -.
DR KEGG; hsa:100101467; -.
DR MANE-Select; ENST00000333206.10; ENSP00000329738.4; NM_001112734.4; NP_001106205.1.
DR UCSC; uc002kyl.5; human. [Q86W11-1]
DR CTD; 100101467; -.
DR DisGeNET; 100101467; -.
DR GeneCards; ZSCAN30; -.
DR HGNC; HGNC:33517; ZSCAN30.
DR HPA; ENSG00000186814; Low tissue specificity.
DR neXtProt; NX_Q86W11; -.
DR OpenTargets; ENSG00000186814; -.
DR PharmGKB; PA165429212; -.
DR VEuPathDB; HostDB:ENSG00000186814; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162162; -.
DR HOGENOM; CLU_002678_49_3_1; -.
DR InParanoid; Q86W11; -.
DR OMA; GMIWKEM; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q86W11; -.
DR TreeFam; TF338304; -.
DR PathwayCommons; Q86W11; -.
DR SignaLink; Q86W11; -.
DR BioGRID-ORCS; 100101467; 12 hits in 1103 CRISPR screens.
DR ChiTaRS; ZSCAN30; human.
DR GenomeRNAi; 100101467; -.
DR Pharos; Q86W11; Tdark.
DR PRO; PR:Q86W11; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q86W11; protein.
DR Bgee; ENSG00000186814; Expressed in sural nerve and 182 other tissues.
DR ExpressionAtlas; Q86W11; baseline and differential.
DR Genevisible; Q86W11; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..494
FT /note="Zinc finger and SCAN domain-containing protein 30"
FT /id="PRO_0000300694"
FT DOMAIN 48..130
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 301..323
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 329..351
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 357..379
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 385..407
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 413..435
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 441..463
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 469..491
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT CROSSLNK 197
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 187..221
FT /note="RMVAGKVLMAKQEIVECVASAAMISPGKLPGETHS -> VSLCHPGWSAVVQ
FT PQLTAVALNPWVKVILLPQPPE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_027861"
FT VAR_SEQ 222..494
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_027862"
FT VARIANT 238
FT /note="Q -> P (in dbSNP:rs2249769)"
FT /id="VAR_059914"
SQ SEQUENCE 494 AA; 56364 MW; 04D07EE957654505 CRC64;
MSGEATVLAY HAPEEQEGLL VVKVEEENYV LDQDFGLQEN PWSQEVFRQK FRQFSYSDST
GPREALSRLR ELCCQWLRPE VHSKEQILEL LMLEQFLAIL PEELQAWLRE HRPENGEEAV
TMLEELEKEL EEPRQQDTTH GQEMFWQEMT STGALKSLSL NSPVQPLENQ CKTETQESQA
FQERDGRMVA GKVLMAKQEI VECVASAAMI SPGKLPGETH SQRIAEEALG GLDNSKKQKG
NAAGNKISQL PSQDRHFSLA TFNRRIPTEH SVLESHESEG SFSMNSNDIT QQSVDTREKL
YECFDCGKAF CQSSKLIRHQ RIHTGERPYA CKECGKAFSL SSDLVRHQRI HSGEKPYECC
ECGKAFRGSS ELIRHRRIHT GEKPYECGEC GKAFSRSSAL IQHKKIHTGD KSYECIACGK
AFGRSSILIE HQRIHTGEKP YECNECGKSF NQSSALTQHQ RIHTGEKPYE CSECRKTFRH
RSGLMQHQRT HTRV
