ZSC32_HUMAN
ID ZSC32_HUMAN Reviewed; 697 AA.
AC Q9NX65; B4DWL5; C9JB03; Q6WMU8; Q7Z6G2; Q8NFX8; Q9BU74;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 3.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Zinc finger and SCAN domain-containing protein 32;
DE AltName: Full=Human cervical cancer suppressor gene 5 protein;
DE Short=HCCS-5;
DE AltName: Full=Zinc finger protein 434;
GN Name=ZSCAN32; Synonyms=ZNF434; ORFNames=HCCS5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Shan Y.X., Luo K.T., Guo Z.K., Tang W.W., Ye G.M., Yu L.;
RT "Cloning and characterization of a human novel znf gene.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Luo K.T., Yu L.;
RT "Cloning and characterization of a novel SCAN box-containing gene.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Kim J.W.;
RT "Identification of a new tumor suppressor gene, HCCS-5.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Mammary gland, and Signet-ring cell carcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-421 (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-152; LYS-163; LYS-400 AND
RP LYS-685, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q9NX65; Q14145: KEAP1; NbExp=3; IntAct=EBI-739949, EBI-751001;
CC Q9NX65; Q9Y6D9: MAD1L1; NbExp=3; IntAct=EBI-739949, EBI-742610;
CC Q9NX65; P57086: SCAND1; NbExp=3; IntAct=EBI-739949, EBI-745846;
CC Q9NX65; Q12933: TRAF2; NbExp=3; IntAct=EBI-739949, EBI-355744;
CC Q9NX65; P17029: ZKSCAN1; NbExp=5; IntAct=EBI-739949, EBI-10199654;
CC Q9NX65; Q969J2: ZKSCAN4; NbExp=5; IntAct=EBI-739949, EBI-2818641;
CC Q9NX65; P17028: ZNF24; NbExp=9; IntAct=EBI-739949, EBI-707773;
CC Q9NX65; Q9NWS9-2: ZNF446; NbExp=4; IntAct=EBI-739949, EBI-740232;
CC Q9NX65; O43309: ZSCAN12; NbExp=5; IntAct=EBI-739949, EBI-1210440;
CC Q9NX65; P10073: ZSCAN22; NbExp=7; IntAct=EBI-739949, EBI-10178224;
CC Q9NX65; Q9NX65: ZSCAN32; NbExp=3; IntAct=EBI-739949, EBI-739949;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9NX65-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NX65-2; Sequence=VSP_046571;
CC Name=3;
CC IsoId=Q9NX65-3; Sequence=VSP_055988;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH02859.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAH02859.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence starting in position 426.; Evidence={ECO:0000305};
CC Sequence=AAM73658.1; Type=Miscellaneous discrepancy; Note=The cDNA sequence has been translated in the reverse direction.; Evidence={ECO:0000305};
CC Sequence=BAA91155.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY261676; AAP57398.1; -; mRNA.
DR EMBL; AF390550; AAM73658.1; ALT_SEQ; mRNA.
DR EMBL; AY279347; AAQ62844.1; -; mRNA.
DR EMBL; AK000424; BAA91155.1; ALT_INIT; mRNA.
DR EMBL; AK291213; BAF83902.1; -; mRNA.
DR EMBL; AK301586; BAG63077.1; -; mRNA.
DR EMBL; AC025283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC002859; AAH02859.1; ALT_SEQ; mRNA.
DR CCDS; CCDS10503.1; -. [Q9NX65-2]
DR CCDS; CCDS66920.1; -. [Q9NX65-3]
DR CCDS; CCDS66921.1; -. [Q9NX65-1]
DR RefSeq; NP_001271456.1; NM_001284527.1. [Q9NX65-1]
DR RefSeq; NP_001271457.1; NM_001284528.1. [Q9NX65-3]
DR RefSeq; NP_001271458.1; NM_001284529.1. [Q9NX65-3]
DR RefSeq; NP_001311269.1; NM_001324340.1. [Q9NX65-2]
DR RefSeq; NP_001311270.1; NM_001324341.1. [Q9NX65-2]
DR RefSeq; NP_001311271.1; NM_001324342.1. [Q9NX65-2]
DR RefSeq; NP_001311273.1; NM_001324344.1. [Q9NX65-2]
DR RefSeq; NP_001311274.1; NM_001324345.1. [Q9NX65-2]
DR RefSeq; NP_060280.2; NM_017810.3. [Q9NX65-2]
DR RefSeq; XP_011520857.1; XM_011522555.2. [Q9NX65-1]
DR RefSeq; XP_016878860.1; XM_017023371.1. [Q9NX65-1]
DR AlphaFoldDB; Q9NX65; -.
DR SMR; Q9NX65; -.
DR BioGRID; 120265; 45.
DR IntAct; Q9NX65; 33.
DR STRING; 9606.ENSP00000380057; -.
DR GlyGen; Q9NX65; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9NX65; -.
DR PhosphoSitePlus; Q9NX65; -.
DR BioMuta; ZSCAN32; -.
DR DMDM; 519668684; -.
DR MassIVE; Q9NX65; -.
DR MaxQB; Q9NX65; -.
DR PaxDb; Q9NX65; -.
DR PeptideAtlas; Q9NX65; -.
DR PRIDE; Q9NX65; -.
DR ProteomicsDB; 5355; -.
DR ProteomicsDB; 79059; -.
DR ProteomicsDB; 83049; -. [Q9NX65-1]
DR Antibodypedia; 1740; 112 antibodies from 19 providers.
DR DNASU; 54925; -.
DR Ensembl; ENST00000304926.7; ENSP00000302502.3; ENSG00000140987.21. [Q9NX65-2]
DR Ensembl; ENST00000396852.9; ENSP00000380061.4; ENSG00000140987.21. [Q9NX65-1]
DR Ensembl; ENST00000439568.2; ENSP00000391787.2; ENSG00000140987.21. [Q9NX65-3]
DR Ensembl; ENST00000618425.4; ENSP00000483210.1; ENSG00000140987.21. [Q9NX65-3]
DR GeneID; 54925; -.
DR KEGG; hsa:54925; -.
DR MANE-Select; ENST00000396852.9; ENSP00000380061.4; NM_001284527.2; NP_001271456.1.
DR UCSC; uc002cux.5; human. [Q9NX65-1]
DR CTD; 54925; -.
DR GeneCards; ZSCAN32; -.
DR HGNC; HGNC:20812; ZSCAN32.
DR HPA; ENSG00000140987; Low tissue specificity.
DR neXtProt; NX_Q9NX65; -.
DR OpenTargets; ENSG00000140987; -.
DR PharmGKB; PA134902932; -.
DR VEuPathDB; HostDB:ENSG00000140987; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000159113; -.
DR HOGENOM; CLU_002678_49_8_1; -.
DR InParanoid; Q9NX65; -.
DR OMA; HFSAHQK; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9NX65; -.
DR TreeFam; TF336839; -.
DR PathwayCommons; Q9NX65; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q9NX65; -.
DR BioGRID-ORCS; 54925; 11 hits in 1100 CRISPR screens.
DR ChiTaRS; ZSCAN32; human.
DR GenomeRNAi; 54925; -.
DR Pharos; Q9NX65; Tdark.
DR PRO; PR:Q9NX65; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9NX65; protein.
DR Bgee; ENSG00000140987; Expressed in right uterine tube and 156 other tissues.
DR ExpressionAtlas; Q9NX65; baseline and differential.
DR Genevisible; Q9NX65; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR044822; Myb_DNA-bind_4.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF13837; Myb_DNA-bind_4; 1.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 6.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..697
FT /note="Zinc finger and SCAN domain-containing protein 32"
FT /id="PRO_0000047582"
FT DOMAIN 37..119
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT DOMAIN 212..296
FT /note="KRAB"
FT ZN_FING 521..543
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 549..571
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 577..599
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 605..627
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 633..655
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 661..683
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 144..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..174
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 379..395
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 152
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 163
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 400
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 685
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..289
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055988"
FT VAR_SEQ 1..212
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.1,
FT ECO:0000303|Ref.3"
FT /id="VSP_046571"
FT VARIANT 266
FT /note="A -> V (in dbSNP:rs17136369)"
FT /id="VAR_052826"
FT VARIANT 332
FT /note="Y -> H (in dbSNP:rs27230)"
FT /id="VAR_024215"
SQ SEQUENCE 697 AA; 78728 MW; AAD779F62506FBCB CRC64;
MMAAVKSTEA HPSSNKDPTQ GQKSALQGNS PDSEASRQRF RQFCYQEVTG PHEAFSKLWE
LCCQWLRPKT HSKEEILELL VLEQFLTILP EEIQTWVREQ HPENGEEAVA LVEDVQRAPG
QQVLDSEKDL KVLMKEMAPL GATRESLRSQ WKQEVQPEEP TFKGSQSSHQ RPGEQSEAWL
APQAPRNLPQ NTGLHDQETG AVVWTAGSQG PAMRDNRAVS LCQQEWMCPG PAQRALYRGA
TQRKDSHVSL ATGVPWGYEE TKTLLAILSS SQFYGKLQTC QQNSQIYRAM AEGLWEQGFL
RTPEQCRTKF KSLQLSYRKV RRGRVPEPCI FYEEMNALSG SWASAPPMAS DAVPGQEGSD
IEAGELNHQN GEPTEVEDGT VDGADRDEKD FRNPGQEVRK LDLPVLFPNR LGFEFKNEIK
KENLKWDDSE EVEINKALQR KSRGVYWHSE LQKGLESEPT SRRQCRNSPG ESEEKTPSQE
KMSHQSFCAR DKACTHILCG KNCSQSVHSP HKPALKLEKV SQCPECGKTF SRSSYLVRHQ
RIHTGEKPHK CSECGKGFSE RSNLTAHLRT HTGERPYQCG QCGKSFNQSS SLIVHQRTHT
GEKPYQCIVC GKRFNNSSQF SAHRRIHTGE SPYKCAVCGK IFNNSSHFSA HRKTHTGEKP
YRCSHCERGF TKNSALTRHQ TVHMKAVLSS QEGRDAL
