ZSWM8_DROME
ID ZSWM8_DROME Reviewed; 2103 AA.
AC Q9VWN9; A0A4D6K2F2; Q95ST9; R9PY27;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Zinc finger SWIM domain-containing protein 8 homolog {ECO:0000305};
DE AltName: Full=Protein dorado {ECO:0000303|PubMed:33184237};
GN ORFNames=CG34401 {ECO:0000312|FlyBase:FBgn0085430};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1345-2103.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=33184237; DOI=10.1126/science.abc9359;
RA Shi C.Y., Kingston E.R., Kleaveland B., Lin D.H., Stubna M.W., Bartel D.P.;
RT "The ZSWIM8 ubiquitin ligase mediates target-directed microRNA
RT degradation.";
RL Science 0:0-0(2020).
CC -!- FUNCTION: Substrate recognition component of a SCF-like E3 ubiquitin-
CC protein ligase complex that promotes target-directed microRNA
CC degradation (TDMD), a process that mediates degradation of microRNAs
CC (miRNAs) (PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase
CC complex acts by catalyzing ubiquitination and subsequent degradation of
CC AGO1, thereby exposing miRNAs for degradation (By similarity).
CC {ECO:0000250|UniProtKB:A7E2V4, ECO:0000269|PubMed:33184237}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:A7E2V4}.
CC -!- SUBUNIT: Component of the SCF-like E3 ubiquitin-protein ligase complex.
CC {ECO:0000250|UniProtKB:A7E2V4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9VWN9-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VWN9-2; Sequence=VSP_060925;
CC Name=C;
CC IsoId=Q9VWN9-3; Sequence=VSP_060926;
CC -!- SIMILARITY: Belongs to the ZSWIM8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL25524.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF48900.3; -; Genomic_DNA.
DR EMBL; AE014298; AAF48899.2; -; Genomic_DNA.
DR EMBL; AE014298; QCD25244.1; -; Genomic_DNA.
DR EMBL; AY060485; AAL25524.1; ALT_INIT; mRNA.
DR RefSeq; NP_573336.3; NM_133108.3. [Q9VWN9-2]
DR RefSeq; NP_652493.2; NM_144236.3. [Q9VWN9-1]
DR AlphaFoldDB; Q9VWN9; -.
DR SMR; Q9VWN9; -.
DR IntAct; Q9VWN9; 2.
DR STRING; 7227.FBpp0111565; -.
DR PaxDb; Q9VWN9; -.
DR PRIDE; Q9VWN9; -.
DR EnsemblMetazoa; FBtr0112653; FBpp0111565; FBgn0085430. [Q9VWN9-1]
DR EnsemblMetazoa; FBtr0333160; FBpp0305363; FBgn0085430. [Q9VWN9-2]
DR EnsemblMetazoa; FBtr0474175; FBpp0423174; FBgn0085430.
DR GeneID; 50356; -.
DR KEGG; dme:Dmel_CG34401; -.
DR UCSC; CG34401-RA; d. melanogaster. [Q9VWN9-1]
DR FlyBase; FBgn0085430; CG34401.
DR VEuPathDB; VectorBase:FBgn0085430; -.
DR eggNOG; KOG3615; Eukaryota.
DR GeneTree; ENSGT00940000156999; -.
DR HOGENOM; CLU_001052_0_0_1; -.
DR InParanoid; Q9VWN9; -.
DR OMA; QMQMYIS; -.
DR OrthoDB; 88229at2759; -.
DR PhylomeDB; Q9VWN9; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 50356; 1 hit in 3 CRISPR screens.
DR ChiTaRS; CG34401; fly.
DR GenomeRNAi; 50356; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0085430; Expressed in wing disc and 20 other tissues.
DR ExpressionAtlas; Q9VWN9; baseline and differential.
DR Genevisible; Q9VWN9; DM.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:FlyBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:2000627; P:positive regulation of miRNA catabolic process; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR007527; Znf_SWIM.
DR Pfam; PF04434; SWIM; 1.
DR PROSITE; PS50966; ZF_SWIM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Reference proteome;
KW Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..2103
FT /note="Zinc finger SWIM domain-containing protein 8
FT homolog"
FT /id="PRO_0000452204"
FT ZN_FING 191..227
FT /note="SWIM-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00325"
FT REGION 684..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1237..1263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1310..1399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1735..1772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1786..1864
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1888..1916
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 684..713
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 722..740
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 759..794
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 801..828
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1310..1357
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1738..1764
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1809..1823
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1832..1861
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1890..1911
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 94
FT /note="Missing (in isoform B)"
FT /id="VSP_060925"
FT VAR_SEQ 2103
FT /note="K -> KQRLDSCNLMTAVATATLATTTAAAGTSATAECKTTTVAASSSSNNS
FT AGNHNNIGNSSSSGVITNNNSSSNGGNINIAISDSVNGHNHHHHHHHHRHHHLLDTPAS
FT SSVGLATATAAGGGGTAAAIPVAATPVSNTPATASSSASAAVAAAAAAAAASAAPSSVA
FT SYERSERRPPLLGAPPALSTVSNNNGRSGAAIAYHSLMPNFYPSTRQLHMHGHARGHAH
FT PPQHGQPHPHQQPQPHSHPHAHPHPHPHPHQYQLQSLSHYHQQL (in isoform
FT C)"
FT /id="VSP_060926"
FT CONFLICT 1791
FT /note="A -> G (in Ref. 3; AAL25524)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2103 AA; 223592 MW; 9AC70B945584F008 CRC64;
MDRFSFDDSI RFEEDSLCSW SSEPESLCNN WRGWKKPAAG SGAGNGGLSG MGGMAVAGGS
GAPPFGGAGA SGSCTPGGNG TAMANTSTCG RHYEVSTLAE LAARCVASYI PFELVEHVYP
PVPEQLQLRI AFWSFPDNEE DIRLYSCLAN SSADEFNRGD SLFRLRAVKD PLQIGFHLSA
SVVNQTPRAY FNVAVTFDRR RISSCNCTCT SSAYWCSHVV AVCLHRIHCP QEVCLRAPVS
ESLTRLQRDQ LQKFAQYLIS ELPQQILPTA QRLLDELLSA QPTAINTVCG APDPTAGASI
NDQTSWYLDE KTLHNNIKRI LIKFCLPAPI VFSDVNYLTN SAPPAAAEWS SLLRPLRGRE
PEGMWNLLSI VREMYRRCDR NAVRLLEIIT EECLYCDQIL IWWFQTKLAL MMGSHGHSGG
KHSNTHSNST ALQHACSSLC DEIVALWRLA ALNPGLAPDE RDMLHAQFTA WHLKILDRVV
KSRMMPSYTN KHQQNSRSET ELFIGFKPAI EACYLDWEGY PIPGVTHTHD TNPIYYSPFT
CFKHTDLKGE SNNPGQLNAT QALMSNNKHY NYFSSSSDHG MHAGAFKQRL DRPFRESRFY
TNPADLDGGA ALAGGGSGRF STGLGSVAVG GAGAGGLGQM SGGVNNSVGG SAAAEVNPVQ
QQLPSGSAGV GASVTNAKVV VATDGNRSSA SSEGFCENDD FGGDTSSSHN YCPPGQAVVP
GQKSALTESD SQSSFDAVSH QSKDEPPVGG VGVAVGVEQA LSTSDTSSAS SSSSSASTAS
GSSNSSTSSM LMAGQEATVG VGGAVQQTTR RLSKDESFSS SSDEFNQGGV GGSAGRVAMA
SSGSGSGAGD TVAVGSGSTA GGDLTPVPST SAAARAALAA SSTGPVISAS PHLAASAAGG
LGVLGVGADQ PCTSSAHAAR ALAAAVAASS DKPHVFSNVR PTEDAWDILL ARAEGLHAHG
HGAEACILAV RLAEQMLANP PNLLLELPPA PKRKGKKQNV NPISHQLTVV ASATLSKCAF
LCTVLSENSE HYHIGFRICL FALEMPRPPA STKPLEVKLA NQEADILALL KRLPLGSAEL
QVIRERAEQL RSGTFKTRGE ALLPINLATF IFDALVTLSP LGGSTIVPGV ASTSGVSSAA
GKSMVNTGTR LLLYKHNSDE SLGFDAAVAA LGLKANVSEA EHPLLCEGTR RQRGDLALTL
LSHYKDEPRK IAKIMEKLLD RDIHTLLNAP LLPAYYSSNP PVRTRSNQPT RREDHDYGGG
SGCASSNCNP VANLCELLPA DYGSVGGNSR PHSSTSAELE LSMCALSMAS SGSQSGGVQG
MVPTTNAAGT TGTPSSSSTT VSGSQNPNGN PSGSGGGGNG GGGNGGGGGG GGGGGGSTSS
RSKESRYKGK RAYPSIPNQP SEASAHFMFE LAKNVLTKAG GNSSTSLFTQ ASTSQNHHGP
HRALHMCAFQ LGLYALGLHN CVSPNWLSRT YSSHVSWILG QAMEIGAPAI SFLIDTWEAH
LTPPEAAGMA DRASRGWDSN MVYPAAELAL SVLPHAAALN PNEIQRAILQ CKEQSDLMLE
RACLTVETAA KGGGVYPEVL FQVARYWYEL YMRNTPNNSE FEPHDDTMDH SAVSLSALIE
SQQQHELQQQ QQAVQQQQAV QQQQQVVQQQ QQVVQQQQQL GMPNSVVPGG VGPGPGLPVA
VQPPVVGSVQ NPQAQFQPVG VASLAPLGLA QYPPYSFCQG LYAHHHNMSY PPGQMQMFIS
AGPPPPPQAY GGYQQPPPQQ PPNPQQQQQV VQQQVQQQQV VQQQQQQVQM AGQAPPGHPG
QQGHPGQPPS GFQPQPPPGA FQALPPQAYQ AMQAGPPGPP MGPPQGYYGP PPPPPPNGPP
GVGVGVGVGV GVGVMPMRQH QHQHPVYPFM QQAPPQPPQQ QQPPPSQPPV RQRQPHQFTP
TQLRYLLAAY NVGMLAMETL ARRVHDDRPQ AKYARNPPYG EDVKWLLRIS KKLGTQYLHQ
FCICAVNSIV SPFVLHDVAI ESAHYLGRNN HQMVMQHLRS ALTPLVQKCQ QMYIQCIHQK
LYHLTQGDYE EFASIVVAAR AAFQITPEGN AQFKDWLQSI KRSKSCKKEL WTQINAALQS
NSK
