ZTF11_CAEEL
ID ZTF11_CAEEL Reviewed; 539 AA.
AC O02274; H2L2J7;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 3.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Zinc finger protein ztf-11 {ECO:0000305};
GN Name=ztf-11 {ECO:0000312|WormBase:F52F12.6a};
GN Synonyms=ekl-2 {ECO:0000312|WormBase:F52F12.6a};
GN ORFNames=F52F12.6 {ECO:0000312|WormBase:F52F12.6a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH LIN-9; LIN-52; RBA-1 AND SIN-3, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=31386623; DOI=10.7554/elife.46703;
RA Lee J., Taylor C.A., Barnes K.M., Shen A., Stewart E.V., Chen A.,
RA Xiang Y.K., Bao Z., Shen K.;
RT "A Myt1 family transcription factor defines neuronal fate by repressing
RT non-neuronal genes.";
RL Elife 8:0-0(2019).
CC -!- FUNCTION: Transcriptional repressor which promotes neuronal
CC differentiation during embryonic and postembryonic neurogenesis
CC (PubMed:31386623). Together with components of the MuvB corepressor
CC complex, negatively regulates the expression of non-neuronal genes
CC during neurogenesis (PubMed:31386623). Required for the generation of
CC postembryonic neurons from epidermal cells (PubMed:31386623).
CC {ECO:0000269|PubMed:31386623}.
CC -!- SUBUNIT: Interacts with MuvB corepressor complex components lin-9, lin-
CC 52 and rba-1; the interaction is required to suppress the activation of
CC non-neuronal genes in neurons (PubMed:31386623). Interacts with sin-3;
CC the interaction is weak (PubMed:31386623).
CC {ECO:0000269|PubMed:31386623}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31386623}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F52F12.6a};
CC IsoId=O02274-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F52F12.6b};
CC IsoId=O02274-2; Sequence=VSP_060462;
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryonic development, but
CC rarely expressed in lineages that do not produce neurons
CC (PubMed:31386623). First expressed in neural precursors at the mid-
CC gastrula stage of embryogenesis (PubMed:31386623). Highly expressed
CC during the late gastrula to lima-bean embryonic stages, but weakly
CC expressed in subsequent embryonic stages (PubMed:31386623). Post
CC hatching, expressed in few neuronal nuclei and transiently expressed in
CC postembryonic neuroectoblasts (PubMed:31386623). Not expressed in
CC postmitotic neurons (PubMed:31386623). In L1 larvae, expressed in
CC neurons and glia, but not in non-neuronal precursors (PubMed:31386623).
CC In L2 stage larvae, expressed in the rectal epithelial Y cell
CC (PubMed:31386623). {ECO:0000269|PubMed:31386623}.
CC -!- DISRUPTION PHENOTYPE: Knockout in the rectal epithelial Y cell results
CC in impaired formation of the PDA motor neuron (PubMed:31386623).
CC Knockout in Pn lineages (the precursor cells of the ventral epidermis
CC of newly hatched animals) results in the defective formation of ventral
CC cord motor neurons (PubMed:31386623). {ECO:0000269|PubMed:31386623}.
CC -!- SIMILARITY: Belongs to the MYT1 family. {ECO:0000305}.
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DR EMBL; BX284601; CAB05737.2; -; Genomic_DNA.
DR EMBL; BX284601; CCE71678.1; -; Genomic_DNA.
DR PIR; T22514; T22514.
DR RefSeq; NP_001251245.1; NM_001264316.1. [O02274-1]
DR RefSeq; NP_001251246.1; NM_001264317.1. [O02274-2]
DR AlphaFoldDB; O02274; -.
DR SMR; O02274; -.
DR STRING; 6239.F52F12.6a; -.
DR EPD; O02274; -.
DR PaxDb; O02274; -.
DR EnsemblMetazoa; F52F12.6a.1; F52F12.6a.1; WBGene00009939. [O02274-1]
DR EnsemblMetazoa; F52F12.6b.1; F52F12.6b.1; WBGene00009939. [O02274-2]
DR GeneID; 172845; -.
DR KEGG; cel:CELE_F52F12.6; -.
DR UCSC; F52F12.6; c. elegans. [O02274-1]
DR CTD; 172845; -.
DR WormBase; F52F12.6a; CE24995; WBGene00009939; ztf-11. [O02274-1]
DR WormBase; F52F12.6b; CE46793; WBGene00009939; ztf-11. [O02274-2]
DR eggNOG; KOG3803; Eukaryota.
DR GeneTree; ENSGT00940000170173; -.
DR HOGENOM; CLU_493673_0_0_1; -.
DR InParanoid; O02274; -.
DR OMA; LMLAQFQ; -.
DR OrthoDB; 1250194at2759; -.
DR PRO; PR:O02274; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00009939; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0002119; P:nematode larval development; IMP:UniProtKB.
DR GO; GO:0048666; P:neuron development; IMP:UniProtKB.
DR GO; GO:0048664; P:neuron fate determination; IMP:UniProtKB.
DR GO; GO:0048665; P:neuron fate specification; IMP:UniProtKB.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:UniProtKB.
DR GO; GO:1903620; P:positive regulation of transdifferentiation; IMP:UniProtKB.
DR InterPro; IPR002515; Znf_C2H2C.
DR InterPro; IPR036060; Znf_C2H2C_sf.
DR Pfam; PF01530; zf-C2HC; 2.
DR SUPFAM; SSF103637; SSF103637; 2.
DR PROSITE; PS51802; ZF_CCHHC; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..539
FT /note="Zinc finger protein ztf-11"
FT /id="PRO_0000448889"
FT ZN_FING 256..299
FT /note="CCHHC-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT ZN_FING 302..345
FT /note="CCHHC-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT REGION 24..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 453..485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 265
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 270
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 283
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 289
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 311
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 316
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 329
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 335
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT VAR_SEQ 1..97
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060462"
SQ SEQUENCE 539 AA; 57069 MW; C8D04C5F3244F1BE CRC64;
MSSISNNPDF SSIDPAVLMS LLMKSSGSLP TPPDTHSDGS ESPDSTASDS SDKKRRRKPE
SKDIVRVAEE AEAAAATCSS IPSSSDTKEN ETEEDQNMTC DTTTNNAQKP TEQTATSADV
VTSSVPSGLE GVPSFLFSQF MAPSFQKQLE IFTSGNMMSA THSDTSPSDV DSVLDGGVVT
AEETSSSKSP MMTSSDTPKT PLTASSPPHS SGSESRVMSP ITHTNISDEL SISTTPTVAF
TPNGSIPSPG TGYSWSIRRE GKLACPTPGC DGSGHQTGLY THHRSLSGCP RRPDKTVIQM
LALRQDTVLR CTTAGCSGKG HVNGNRTSHR SLSGCPIAHQ EKLARKGIKT TPQRTKTPIK
GISISDECPL DLTLSGLPAG LSAQQLLAAA QAGLIPSSQM MDALFQQFSQ TQPLATLEEE
SKKENEMEVD VETTSDDIPT LIKEEEEVKC ESPVPSVIPE IQSTPSRPVA APVAPGSAEK
SSPTSQMLLQ MPGFSEALLK MTAPQVPFPQ YSPQAALFGN QSALLAQIML TQLQMQQGF
