ID   ZW10_ARATH              Reviewed;         742 AA.
AC   O48626; O48775; Q8GY19;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Centromere/kinetochore protein zw10 homolog;
DE            Short=AtZW10;
DE   AltName: Full=MAG2-interacting protein 1 {ECO:0000303|PubMed:24118572};
GN   Name=ZW10; Synonyms=MIP1 {ECO:0000303|PubMed:24118572};
GN   OrderedLocusNames=At2g32900 {ECO:0000312|Araport:AT2G32900};
GN   ORFNames=T21L14.16 {ECO:0000312|EMBL:AAB91977.2};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root;
RX   PubMed=9298984; DOI=10.1083/jcb.138.6.1289;
RA   Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K.,
RA   Goldberg M.L.;
RT   "Conservation of the centromere/kinetochore protein ZW10.";
RL   J. Cell Biol. 138:1289-1301(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   INTERACTION WITH MAG2.
RX   PubMed=23025793; DOI=10.1111/j.1399-3054.2012.01704.x;
RA   Zhao P., Liu F., Zhang B., Liu X., Wang B., Gong J., Yu G., Ma M., Lu Y.,
RA   Sun J., Wang Z., Jia P., Liu H.;
RT   "MAIGO2 is involved in abscisic acid-mediated response to abiotic stresses
RT   and Golgi-to-ER retrograde transport.";
RL   Physiol. Plantarum 148:246-260(2013).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION,
RP   INTERACTION WITH MAG2, AND DISRUPTION PHENOTYPE.
RX   PubMed=24118572; DOI=10.1111/tpj.12347;
RA   Li L., Shimada T., Takahashi H., Koumoto Y., Shirakawa M., Takagi J.,
RA   Zhao X., Tu B., Jin H., Shen Z., Han B., Jia M., Kondo M., Nishimura M.,
RA   Hara-Nishimura I.;
RT   "MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex to
RT   facilitate storage protein transport in Arabidopsis thaliana.";
RL   Plant J. 76:781-791(2013).
CC   -!- FUNCTION: May be required for accurate chromosome segregation. Required
CC       for proper maturation of seed storage proteins. Forms a complex with
CC       MAG2, MIP2 and MIP3 on the endoplasmic reticulum that may be
CC       responsible for efficient transport of seed storage proteins.
CC       {ECO:0000269|PubMed:24118572, ECO:0000305}.
CC   -!- SUBUNIT: Interacts (via the central region) with MAG2
CC       (PubMed:23025793). Forms a complex with MAG2, MIP2 and MIP3 on the
CC       endoplasmic reticulum (PubMed:24118572). {ECO:0000269|PubMed:23025793,
CC       ECO:0000269|PubMed:24118572}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle. Cytoplasm.
CC       Chromosome, centromere. Chromosome, centromere, kinetochore.
CC       Endoplasmic reticulum membrane; Peripheral membrane protein
CC       {ECO:0000269|PubMed:24118572}. Note=In prometaphase, associated with
CC       the kinetochore. At metaphase, mostly associated with the spindle. In
CC       early anaphase, found in the vicinity of the centromere. As anaphase
CC       progresses, dispersed in the cytoplasm.
CC   -!- DISRUPTION PHENOTYPE: Accumulation of the precursors of the two major
CC       storage proteins albumin 2S and globulin 12S in dry seeds.
CC       {ECO:0000269|PubMed:24118572}.
CC   -!- SIMILARITY: Belongs to the ZW10 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB91977.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; U80984; AAB88246.1; -; mRNA.
DR   EMBL; AC003033; AAB91977.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC08759.1; -; Genomic_DNA.
DR   EMBL; AK117917; BAC42555.1; -; mRNA.
DR   EMBL; AY069883; AAL47437.1; -; mRNA.
DR   PIR; T01117; T01117.
DR   RefSeq; NP_565757.2; NM_128850.3.
DR   AlphaFoldDB; O48626; -.
DR   SMR; O48626; -.
DR   BioGRID; 3198; 3.
DR   STRING; 3702.AT2G32900.1; -.
DR   PaxDb; O48626; -.
DR   PRIDE; O48626; -.
DR   ProteomicsDB; 232302; -.
DR   EnsemblPlants; AT2G32900.1; AT2G32900.1; AT2G32900.
DR   GeneID; 817851; -.
DR   Gramene; AT2G32900.1; AT2G32900.1; AT2G32900.
DR   KEGG; ath:AT2G32900; -.
DR   Araport; AT2G32900; -.
DR   TAIR; locus:2059412; AT2G32900.
DR   eggNOG; KOG2163; Eukaryota.
DR   HOGENOM; CLU_012948_1_0_1; -.
DR   InParanoid; O48626; -.
DR   OMA; LMMILQA; -.
DR   OrthoDB; 422807at2759; -.
DR   PhylomeDB; O48626; -.
DR   PRO; PR:O48626; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O48626; baseline and differential.
DR   Genevisible; O48626; AT.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:InterPro.
DR   GO; GO:1990423; C:RZZ complex; IBA:GO_Central.
DR   GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:TAIR.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0032527; P:protein exit from endoplasmic reticulum; IMP:TAIR.
DR   GO; GO:0015031; P:protein transport; IMP:TAIR.
DR   Gene3D; 1.10.357.150; -; 1.
DR   InterPro; IPR009361; RZZ-complex_Zw10.
DR   InterPro; IPR046362; Zw10/DSL1_C_sf.
DR   Pfam; PF06248; Zw10; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Endoplasmic reticulum; Kinetochore; Membrane; Mitosis; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..742
FT                   /note="Centromere/kinetochore protein zw10 homolog"
FT                   /id="PRO_0000184963"
FT   CONFLICT        702
FT                   /note="E -> G (in Ref. 4; BAC42555)"
SQ   SEQUENCE   742 AA;  83934 MW;  81C31987882FDD22 CRC64;
     MPEIDALFES INVRDLLAGH DLNDPTTPLS APDLRLLINR LESHSLRIKS KVQSYLVAHH
     SDFSELFSLC QDTVSRTRLI SDDVSDVLQL VSDRPIDVEI RSVVDEITEK TKEVKLKRES
     LDLVNAIVGI CEALQETKEA LKNGRFRFAA ERIRELKVVL RIGEEEDGEP VAYALLRKEW
     SNCFDEIQEV LAKFMENAVR FELDSSRIRI KYQLSVGETA GIALSTVLEA MEVIGILDYG
     LAKAADSIFK HVITPAVTHA STFAAVEDLC KSAGEVTEAT LRLEQSSDHK FEDVDGDAMY
     SGILKVVKFI CSSLCFGNVT WIHSFGRLTW PRISELIISK FLSKVVPEDA SKLADFQKII
     ERTSQFEAAL KELNFVSSSD AESRLSKYAE DVEVHFASRK KIEILAKARN LLLQCNFTIP
     QDIAMRNAKH IVCLLFSSER CVVSEAASQL MNLVHKTLED VCVSSARVAS EFYNAARDSI
     LLYEAVVPVK LEKQLDGLNE AAVLLHNDCL YLFEEILGLA FEYRASFPSS IKEYAVFADI
     APRFKLMAEE VLQKQVHLVI SSLREAIDSA DGFQNTHQIK QFKSAEFSID QVVFSLKNVH
     MIWEPVLRPK TYKQSMCAVL ESVFRRIARD ILLLDDMAAD ETFELQKLIY LMLKNLSSVL
     DSVRSADETS RPLDDIIPSL RKTRKLAELL DMPLMSITSA WESGELFRCN FTRTEVQDFI
     KAIFTDSPLR KECLWRIDEV NQ