ZW10_CAEEL
ID ZW10_CAEEL Reviewed; 778 AA.
AC Q19642;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Centromere/kinetochore protein zw10 homolog {ECO:0000305};
GN Name=czw-1 {ECO:0000303|PubMed:9298984, ECO:0000312|WormBase:F20D12.4};
GN ORFNames=F20D12.4 {ECO:0000312|WormBase:F20D12.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9298984; DOI=10.1083/jcb.138.6.1289;
RA Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K.,
RA Goldberg M.L.;
RT "Conservation of the centromere/kinetochore protein ZW10.";
RL J. Cell Biol. 138:1289-1301(1997).
RN [3] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN RZZ COMPLEX, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=18765790; DOI=10.1101/gad.1687508;
RA Gassmann R., Essex A., Hu J.-S., Maddox P.S., Motegi F., Sugimoto A.,
RA O'Rourke S.M., Bowerman B., McLeod I., Yates J.R. III, Oegema K.,
RA Cheeseman I.M., Desai A.;
RT "A new mechanism controlling kinetochore-microtubule interactions revealed
RT by comparison of two dynein-targeting components: SPDL-1 and the
RT Rod/Zwilch/Zw10 complex.";
RL Genes Dev. 22:2385-2399(2008).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18936247; DOI=10.1083/jcb.200805185;
RA Yamamoto T.G., Watanabe S., Essex A., Kitagawa R.;
RT "SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis
RT elegans.";
RL J. Cell Biol. 183:187-194(2008).
RN [5] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=19109417; DOI=10.1091/mbc.e08-10-1047;
RA Essex A., Dammermann A., Lewellyn L., Oegema K., Desai A.;
RT "Systematic analysis in Caenorhabditis elegans reveals that the spindle
RT checkpoint is composed of two largely independent branches.";
RL Mol. Biol. Cell 20:1252-1267(2009).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=24231804; DOI=10.1126/science.1246232;
RA Cheerambathur D.K., Gassmann R., Cook B., Oegema K., Desai A.;
RT "Crosstalk between microtubule attachment complexes ensures accurate
RT chromosome segregation.";
RL Science 342:1239-1242(2013).
CC -!- FUNCTION: Essential component of the mitotic checkpoint, which prevents
CC cells from prematurely exiting mitosis (PubMed:9298984). Required for
CC the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto
CC kinetochores (PubMed:18936247). Its function related to the spindle
CC assembly machinery and kinetochore-microtubule attachments likely
CC depends on its association in the mitotic RZZ complex
CC (PubMed:18936247). The RZZ complex recruits the spindly-like protein
CC spdl-1 to kinetochores (PubMed:18765790, PubMed:18936247). To prevent
CC irregular chromosome segregation, the complex also inhibits the
CC attachment of the kinetochore-associated NDC80 complex to microtubules
CC (PubMed:24231804). The recruitment of spdl-1 to kinetochores relieves
CC this inhibition (PubMed:24231804). Required for embryonic development
CC (PubMed:9298984). {ECO:0000269|PubMed:18765790,
CC ECO:0000269|PubMed:18936247, ECO:0000269|PubMed:9298984,
CC ECO:0000305|PubMed:24231804}.
CC -!- SUBUNIT: Component of the RZZ complex composed of rod-1, czw-1 and zwl-
CC 1. {ECO:0000269|PubMed:18765790}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:18936247}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000250|UniProtKB:O43264}. Note=Localizes to
CC the kinetochore during nuclear envelope breakdown and remains there
CC until the metaphase-anaphase transition. Localization of the RZZ
CC complex to kinetochores is dependent upon knl-1.
CC {ECO:0000269|PubMed:18765790}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown largely results in
CC sterility (PubMed:9298984, PubMed:18765790, PubMed:19109417). RNAi-
CC mediated knockdown also results in a high incidence of embryonic
CC lethality in the embryos produced and defects in mitosis which include
CC the formation of chromatin bridges in between sister chromatids during
CC anaphase (PubMed:9298984). In addition, there is reduced localization
CC of the spindly-like protein spdl-1 to kinetochores, but not to
CC microtubules (PubMed:18936247). {ECO:0000269|PubMed:18765790,
CC ECO:0000269|PubMed:18936247, ECO:0000269|PubMed:19109417,
CC ECO:0000269|PubMed:9298984}.
CC -!- SIMILARITY: Belongs to the ZW10 family. {ECO:0000305}.
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DR EMBL; BX284604; CCD67719.1; -; Genomic_DNA.
DR PIR; T16111; T16111.
DR RefSeq; NP_501327.1; NM_068926.4.
DR AlphaFoldDB; Q19642; -.
DR SMR; Q19642; -.
DR ComplexPortal; CPX-810; Rzz complex.
DR STRING; 6239.F20D12.4.1; -.
DR EPD; Q19642; -.
DR PaxDb; Q19642; -.
DR PeptideAtlas; Q19642; -.
DR EnsemblMetazoa; F20D12.4.1; F20D12.4.1; WBGene00017643.
DR EnsemblMetazoa; F20D12.4.2; F20D12.4.2; WBGene00017643.
DR GeneID; 177587; -.
DR KEGG; cel:CELE_F20D12.4; -.
DR UCSC; F20D12.4.1; c. elegans.
DR CTD; 177587; -.
DR WormBase; F20D12.4; CE04434; WBGene00017643; czw-1.
DR eggNOG; KOG2163; Eukaryota.
DR GeneTree; ENSGT00390000016427; -.
DR HOGENOM; CLU_012948_0_0_1; -.
DR InParanoid; Q19642; -.
DR OMA; MMNASLK; -.
DR OrthoDB; 422807at2759; -.
DR PhylomeDB; Q19642; -.
DR Reactome; R-CEL-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:Q19642; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00017643; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:1990423; C:RZZ complex; IDA:UniProtKB.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR GO; GO:0034501; P:protein localization to kinetochore; IDA:ComplexPortal.
DR GO; GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR Gene3D; 1.10.357.150; -; 1.
DR InterPro; IPR009361; RZZ-complex_Zw10.
DR InterPro; IPR046362; Zw10/DSL1_C_sf.
DR Pfam; PF06248; Zw10; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Mitosis;
KW Reference proteome.
FT CHAIN 1..778
FT /note="Centromere/kinetochore protein zw10 homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438725"
FT COILED 47..99
FT /evidence="ECO:0000255"
SQ SEQUENCE 778 AA; 87993 MW; 183629EA2708B34B CRC64;
MASSEGSYRD LEKKLKNGLT SISQDIVDKY GNLKVSLNVN ATAKLIFDRL ENLEDIAEMS
TRNLSNLIDQ TAKDSPEMLA EIKSQAQSCE NLVEFLQSMK NVEEQLIIMR SKTTNRVEWG
TAILACKDFL NDTNMLLEGI GRDGFDMSVP LKHFAAEYSV LSYNCRYQLS ADYERAMNVP
KLSKQKCGDR TNVSFSVFNV GSVEDQKMLN ETLSAMNMIG QLPERLDAWK IVILNVFCEA
IVASRDGVDV YIVDNPTPDQ TRFLINQKPR GKKDKTIDVA KVLESMEVFF TKLHSVLHSH
ELLDATGKTF TSMIGSVIEE QLITMILKDV IAIAAPVTET ADEDQEMFIN LLQIGEVFVE
RMKELGFFSQ KAKLLFTLDT DTIFVTRRCF AIVSKANKLI NETYDKLVTV GVDDSAIKDI
DLLAKAHTHA EHFAKEYGKD LGRLWSHNED SQFPSFFAFQ KCTVSESTIN FVNLLRDNVK
AAFACEDEGA RAKLALTAEN IVRLYVILTP RKHAELFSSI PNMAAIFYNN CHYISHCIMT
MSFEASGDNQ KTLLEPLLLD SVIRLRTVAA DCMEKTLTRC RREMTAYLED HSIFEHLPAS
YKTTKNTFAA AEEMSESADI LVPREEPKII KCLAACLLHI RLIAKNLREP LTEVVYCKVI
GSLVSFLLDS LVRHVVTTSD FRENDANVMA DVFKRLLEVV ANIVAYKEQT KVTDFCAREY
FRLNEIVFVL GNRMQDIEHR WFNAKGPMAE HLSRSEVVGL IKALFADSQH RSDLIARL
