ID   ZWILC_CAEBR             Reviewed;         639 AA.
AC   P0C664; A8WVW7;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Protein zwilch homolog;
GN   Name=zwl-1; ORFNames=CBG03981;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Essential component of the mitotic checkpoint, which prevents
CC       cells from prematurely exiting mitosis. Required for chromosome
CC       segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2
CC       complexes onto kinetochores and spindle pole separation. Its function
CC       related to the spindle assembly machinery and kinetochore-microtubule
CC       attachments likely depends on its association in the mitotic RZZ
CC       complex. The RZZ complex recruits the spindly-like protein spdl-1 to
CC       kinetochores. To prevent irregular chromosome segregation, the complex
CC       also inhibits the attachment of the kinetochore-associated NDC80
CC       complex to microtubules. The recruitment of spdl-1 to kinetochores
CC       relieves this inhibition. Required for embryonic development.
CC       {ECO:0000250|UniProtKB:Q95XP9}.
CC   -!- SUBUNIT: Component of the RZZ complex composed of rod-1, czw-1 and zwl-
CC       1. Interacts with the spindly-like protein spdl-1. Interacts with NDC80
CC       complex component ndc-80. {ECO:0000250|UniProtKB:Q95XP9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC       {ECO:0000250|UniProtKB:Q95XP9}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q95XP9}. Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q95XP9}. Note=Localizes to the kinetochore
CC       during nuclear envelope breakdown and remains there until the
CC       metaphase-anaphase transition. Localizes to kinetochores, spindle-
CC       associated rod-shaped structures and near the cell cortex in a knl-1-
CC       dependent manner in anaphase. {ECO:0000250|UniProtKB:Q95XP9}.
CC   -!- SIMILARITY: Belongs to the ZWILCH family. {ECO:0000305}.
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DR   EMBL; HE600906; CAP24780.1; -; Genomic_DNA.
DR   RefSeq; XP_002639393.1; XM_002639347.1.
DR   AlphaFoldDB; P0C664; -.
DR   STRING; 6238.CBG03981; -.
DR   EnsemblMetazoa; CBG03981.1; CBG03981.1; WBGene00026734.
DR   GeneID; 8581386; -.
DR   KEGG; cbr:CBG_03981; -.
DR   CTD; 8581386; -.
DR   WormBase; CBG03981; CBP06615; WBGene00026734; Cbr-zwl-1.
DR   eggNOG; KOG4803; Eukaryota.
DR   HOGENOM; CLU_431650_0_0_1; -.
DR   InParanoid; P0C664; -.
DR   OMA; MMEIGVE; -.
DR   OrthoDB; 669333at2759; -.
DR   Proteomes; UP000008549; Chromosome I.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:1990423; C:RZZ complex; IBA:GO_Central.
DR   GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:UniProtKB.
DR   GO; GO:0010696; P:positive regulation of mitotic spindle pole body separation; IEA:EnsemblMetazoa.
DR   GO; GO:1905342; P:positive regulation of protein localization to kinetochore; IEA:EnsemblMetazoa.
DR   GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR   GO; GO:1903394; P:protein localization to kinetochore involved in kinetochore assembly; IEA:EnsemblMetazoa.
DR   InterPro; IPR018630; Zwilch.
DR   PANTHER; PTHR15995; PTHR15995; 1.
DR   Pfam; PF09817; Zwilch; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Developmental protein; Kinetochore; Meiosis; Mitosis; Reference proteome.
FT   CHAIN           1..639
FT                   /note="Protein zwilch homolog"
FT                   /id="PRO_0000314806"
FT   REGION          76..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        78..99
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   639 AA;  72283 MW;  0BBE3EF5A8661437 CRC64;
     MSIKLEDLHS FNEAVLFKGE EDEAPAPVLL LDKYRVRLVP ITELPLVSNY SNTSQLGLNS
     EEVLVIDSPI ESAEKQKTSS LLNRRENKKT IKSEKEDESM DMETAEGDKE NTVSETGGGP
     LVTSFLTLDK LEKDGVNDVE IVGLDCEIQF FDANPIPFED GISLQRFLRL ESSKNFSAAV
     DKLPIWISTI GHHFPSVCWL AAGRTNKNVQ VSGATRILGY FNENSQKLVK QLNEACGAAQ
     VNRYRAVYDV IRKIATPTRE APGEVIIDMR WNTKSSLVLL EQPDNAADCT IKIDLGWGDN
     RFFIDETIFE QLFFVLNLAD VLANPEKEVV FRSESDKFDD LVQEMKQLVE ACSHEDNVFA
     SNEKSEQVTD KVWNIVRKCS DVKQATMLFK NFLQALTYGK IKSHVQEGNK SHLASLIRAS
     KTCDFRMPIL ERLSTIEMMM EIGVESLRGR IINKFSDTLQ FPSDELTFIL KTCENDLSTL
     EGTLHSSVVS LLPITMAMAT IHQIFGFLNV KDLVVLPDLA RRVLTKYTTG MVEKAKRGET
     ETDYVFETTL PLLRMNKEAF MHKRPRIWTC ENTNTVGANV QTRAMTTLEL EPSLEHVCRL
     VNASRPVRPI DEDNRKPTEE ERNADYTVSH TIFSYLPKL