ZWILC_CAEEL
ID ZWILC_CAEEL Reviewed; 630 AA.
AC Q95XP9; A0A061AD83; A0A061AEC9; H2KZV1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Protein zwilch homolog;
GN Name=zwl-1; ORFNames=Y39G10AR.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, IDENTIFICATION IN RZZ COMPLEX, INTERACTION WITH SPDL-1,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18765790; DOI=10.1101/gad.1687508;
RA Gassmann R., Essex A., Hu J.-S., Maddox P.S., Motegi F., Sugimoto A.,
RA O'Rourke S.M., Bowerman B., McLeod I., Yates J.R. III, Oegema K.,
RA Cheeseman I.M., Desai A.;
RT "A new mechanism controlling kinetochore-microtubule interactions revealed
RT by comparison of two dynein-targeting components: SPDL-1 and the
RT Rod/Zwilch/Zw10 complex.";
RL Genes Dev. 22:2385-2399(2008).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19109417; DOI=10.1091/mbc.e08-10-1047;
RA Essex A., Dammermann A., Lewellyn L., Oegema K., Desai A.;
RT "Systematic analysis in Caenorhabditis elegans reveals that the spindle
RT checkpoint is composed of two largely independent branches.";
RL Mol. Biol. Cell 20:1252-1267(2009).
RN [4]
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=20729837; DOI=10.1038/ncb2093;
RA Dumont J., Oegema K., Desai A.;
RT "A kinetochore-independent mechanism drives anaphase chromosome separation
RT during acentrosomal meiosis.";
RL Nat. Cell Biol. 12:894-901(2010).
RN [5]
RP FUNCTION, AND INTERACTION WITH NDC-80.
RX PubMed=24231804; DOI=10.1126/science.1246232;
RA Cheerambathur D.K., Gassmann R., Cook B., Oegema K., Desai A.;
RT "Crosstalk between microtubule attachment complexes ensures accurate
RT chromosome segregation.";
RL Science 342:1239-1242(2013).
CC -!- FUNCTION: Essential component of the mitotic checkpoint, which prevents
CC cells from prematurely exiting mitosis (PubMed:18765790). Required for
CC chromosome segregation, the assembly of the dynein-dynactin and mdf-1-
CC mdf-2 complexes onto kinetochores and spindle pole separation
CC (PubMed:18765790). Its function related to the spindle assembly
CC machinery and kinetochore-microtubule attachments likely depends on its
CC association in the mitotic RZZ complex (PubMed:18765790). The RZZ
CC complex recruits the spindly-like protein spdl-1 to kinetochores
CC (PubMed:18765790). To prevent irregular chromosome segregation, the
CC complex also inhibits the attachment of the kinetochore-associated
CC NDC80 complex to microtubules (PubMed:24231804). The recruitment of
CC spdl-1 to kinetochores relieves this inhibition (PubMed:24231804).
CC Required for embryonic development (PubMed:18765790, PubMed:19109417).
CC {ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:19109417,
CC ECO:0000269|PubMed:24231804}.
CC -!- SUBUNIT: Component of the RZZ complex composed of rod-1, czw-1 and zwl-
CC 1 (PubMed:18765790). Interacts with the spindly-like protein spdl-1
CC (PubMed:18765790). Interacts with NDC80 complex component ndc-80
CC (PubMed:24231804). {ECO:0000269|PubMed:18765790,
CC ECO:0000269|PubMed:24231804}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:20729837}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:19109417,
CC ECO:0000269|PubMed:20729837}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000305}. Note=Localizes to the kinetochore during nuclear
CC envelope breakdown and remains there until the metaphase-anaphase
CC transition (PubMed:18765790). Localizes to kinetochores, spindle-
CC associated rod-shaped structures and near the cell cortex in a knl-1-
CC dependent manner in anaphase (PubMed:18765790, PubMed:20729837).
CC {ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:20729837,
CC ECO:0000305|PubMed:18765790}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a {ECO:0000312|WormBase:Y39G10AR.2a};
CC IsoId=Q95XP9-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y39G10AR.2b};
CC IsoId=Q95XP9-2; Sequence=VSP_057650;
CC Name=c {ECO:0000312|WormBase:Y39G10AR.2c};
CC IsoId=Q95XP9-3; Sequence=VSP_057649;
CC Name=d {ECO:0000312|WormBase:Y39G10AR.2d};
CC IsoId=Q95XP9-4; Sequence=VSP_057649, VSP_057650;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality (PubMed:18765790, PubMed:19109417). RNAi-mediated knockdown
CC results in defective chromosome segregation, premature spindle pole
CC separation and incorrectly attached kinetochores (PubMed:18765790). In
CC addition, knl-1 localization to the spindle-associated rod-shaped
CC structures but not to kinetochores is lost during anaphase of meiosis I
CC (PubMed:20729837). RNAi-mediated knockdown also results in abolished
CC targeting of the spindly-like protein spdl-1 to the kinetochore
CC (PubMed:18765790). {ECO:0000269|PubMed:18765790,
CC ECO:0000269|PubMed:19109417, ECO:0000269|PubMed:20729837}.
CC -!- SIMILARITY: Belongs to the ZWILCH family. {ECO:0000305}.
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DR EMBL; FO081209; CCD69901.1; -; Genomic_DNA.
DR EMBL; FO081209; CCE67248.1; -; Genomic_DNA.
DR EMBL; FO081209; CDR32818.1; -; Genomic_DNA.
DR EMBL; FO081209; CDR32819.1; -; Genomic_DNA.
DR RefSeq; NP_001249150.1; NM_001262221.1. [Q95XP9-1]
DR RefSeq; NP_001249151.1; NM_001262222.1. [Q95XP9-2]
DR RefSeq; NP_001293318.1; NM_001306389.1. [Q95XP9-3]
DR RefSeq; NP_001293319.1; NM_001306390.1. [Q95XP9-4]
DR AlphaFoldDB; Q95XP9; -.
DR BioGRID; 37283; 10.
DR ComplexPortal; CPX-810; Rzz complex.
DR STRING; 6239.Y39G10AR.2a; -.
DR EPD; Q95XP9; -.
DR PaxDb; Q95XP9; -.
DR PeptideAtlas; Q95XP9; -.
DR EnsemblMetazoa; Y39G10AR.2a.1; Y39G10AR.2a.1; WBGene00021460. [Q95XP9-1]
DR EnsemblMetazoa; Y39G10AR.2b.1; Y39G10AR.2b.1; WBGene00021460. [Q95XP9-2]
DR EnsemblMetazoa; Y39G10AR.2c.1; Y39G10AR.2c.1; WBGene00021460. [Q95XP9-3]
DR EnsemblMetazoa; Y39G10AR.2d.1; Y39G10AR.2d.1; WBGene00021460. [Q95XP9-4]
DR GeneID; 171798; -.
DR KEGG; cel:CELE_Y39G10AR.2; -.
DR UCSC; Y39G10AR.2; c. elegans. [Q95XP9-1]
DR CTD; 171798; -.
DR WormBase; Y39G10AR.2a; CE26992; WBGene00021460; zwl-1. [Q95XP9-1]
DR WormBase; Y39G10AR.2b; CE46826; WBGene00021460; zwl-1. [Q95XP9-2]
DR WormBase; Y39G10AR.2c; CE49916; WBGene00021460; zwl-1. [Q95XP9-3]
DR WormBase; Y39G10AR.2d; CE49848; WBGene00021460; zwl-1. [Q95XP9-4]
DR eggNOG; KOG4803; Eukaryota.
DR GeneTree; ENSGT00390000013696; -.
DR InParanoid; Q95XP9; -.
DR OMA; MMEIGVE; -.
DR OrthoDB; 669333at2759; -.
DR PhylomeDB; Q95XP9; -.
DR PRO; PR:Q95XP9; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00021460; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR GO; GO:1990423; C:RZZ complex; IDA:UniProtKB.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0010696; P:positive regulation of mitotic spindle pole body separation; IMP:UniProtKB.
DR GO; GO:1905342; P:positive regulation of protein localization to kinetochore; IMP:UniProtKB.
DR GO; GO:0034501; P:protein localization to kinetochore; IDA:ComplexPortal.
DR GO; GO:1903394; P:protein localization to kinetochore involved in kinetochore assembly; IMP:UniProtKB.
DR GO; GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR InterPro; IPR018630; Zwilch.
DR PANTHER; PTHR15995; PTHR15995; 1.
DR Pfam; PF09817; Zwilch; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW Cytoplasm; Cytoskeleton; Developmental protein; Kinetochore; Meiosis;
KW Mitosis; Reference proteome.
FT CHAIN 1..630
FT /note="Protein zwilch homolog"
FT /id="PRO_0000314807"
FT VAR_SEQ 1..321
FT /note="Missing (in isoform c and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057649"
FT VAR_SEQ 354..356
FT /note="NFR -> K (in isoform b and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057650"
SQ SEQUENCE 630 AA; 71096 MW; A5E4373C5E262928 CRC64;
MPLTIEQLKQ YNEKMAAKGE EDDAPAPVLL LDKYRVRVLP LTSIPFVMNH SSVSQLSLSS
EDVLVVDFPS KGTGSGAGKA EKKMIFKKKI EPMEDSFEDK ENVNEGGPLM TSFLTLEKLR
KGMVGDVEII GYECETSFFD ANPIPLSDGV SLQRYIRLNC SEHFSPSIST LPVWISTTSS
KFPSICWLAA GRTNRNVQFA AATRVLGHFN NENSERVVKQ LNQACGTSQL NKYRAVYEEI
RKIATENRPA PGEVTIDVRW STKSTLVLLE HPDNAADCTI KIDLGWGDKR FFVDDEIFEQ
LFFVLNLADV LANPDNEVIF PMAPPANFDD LVQEMDALVE ASSREDNVFV SNENFRGGDI
TDKVWNIVRK CNDVKQVTLL FRNFLQALAY GKIKSHAQER NKSHLASLIR ISKSSEFKIP
VLERLSTIDM MMEIGVESLR RRVIDIFSSQ LLYPSDELEF ILQTCENDLP AGNGAMNSAA
ISLLPITMAL ATANRIYELL NEKDHVILPD LTRRILQKYT ASMIEKSRRG ETETEYTFET
TLPLLRMYKE GFMSKRPCIW TCENSNTVGA NVQARVLTSL ELQPSLEHIN RLVNDSRPVW
TATEEKPAMI KDLNADYTVV HTIFSYLPKM
