ID   ZWILC_DANRE             Reviewed;         583 AA.
AC   A5WWB6; Q1JQ30; Q501W8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Protein zwilch homolog;
GN   Name=zwilch; ORFNames=si:ch211-242m18.4, si:ch73-94f11.1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo, and Ovary;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential component of the mitotic checkpoint, which prevents
CC       cells from prematurely exiting mitosis. Required for the assembly of
CC       the dynein-dynactin and mad1-mad2 complexes onto kinetochores (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RZZ complex composed of kntc1/rod, zw10 and
CC       zwilch. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ZWILCH family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH95837.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAI16507.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAI17615.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CT863714; CAN88106.1; -; Genomic_DNA.
DR   EMBL; CT954222; CAN88106.1; JOINED; Genomic_DNA.
DR   EMBL; CT954222; CAN88279.1; -; Genomic_DNA.
DR   EMBL; CT863714; CAN88279.1; JOINED; Genomic_DNA.
DR   EMBL; BC095837; AAH95837.1; ALT_INIT; mRNA.
DR   EMBL; BC116506; AAI16507.1; ALT_INIT; mRNA.
DR   EMBL; BC117614; AAI17615.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001186664.1; NM_001199735.1.
DR   AlphaFoldDB; A5WWB6; -.
DR   SMR; A5WWB6; -.
DR   STRING; 7955.ENSDARP00000074147; -.
DR   PaxDb; A5WWB6; -.
DR   PeptideAtlas; A5WWB6; -.
DR   Ensembl; ENSDART00000079695; ENSDARP00000074147; ENSDARG00000057100.
DR   GeneID; 336439; -.
DR   KEGG; dre:336439; -.
DR   CTD; 55055; -.
DR   ZFIN; ZDB-GENE-050420-101; zwilch.
DR   eggNOG; KOG4803; Eukaryota.
DR   GeneTree; ENSGT00390000013696; -.
DR   HOGENOM; CLU_466141_0_0_1; -.
DR   InParanoid; A5WWB6; -.
DR   OMA; CAAEEFY; -.
DR   OrthoDB; 1451737at2759; -.
DR   PhylomeDB; A5WWB6; -.
DR   TreeFam; TF324453; -.
DR   Reactome; R-DRE-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-DRE-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DRE-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-DRE-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-DRE-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:A5WWB6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 18.
DR   Bgee; ENSDARG00000057100; Expressed in testis and 27 other tissues.
DR   GO; GO:1990423; C:RZZ complex; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:UniProtKB.
DR   GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR   InterPro; IPR018630; Zwilch.
DR   PANTHER; PTHR15995; PTHR15995; 1.
DR   Pfam; PF09817; Zwilch; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Mitosis;
KW   Reference proteome.
FT   CHAIN           1..583
FT                   /note="Protein zwilch homolog"
FT                   /id="PRO_0000314803"
FT   CONFLICT        499
FT                   /note="I -> V (in Ref. 2; AAH95837)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   583 AA;  66078 MW;  77923FD0D43A45AB CRC64;
     MGSKIVSDAN AFVKFLKLCQ DESKDNCTYM DDVSVLLVKK EQLLHDIIGE KNQPIFICEK
     AQTKYEPEAT ETSADTSDVE DVLFKVEQDP GPQPLSIMKA RQLLSWYTMA HNPNMSRLKA
     PENLHPLWVR CDKSDPCATA WLGVEITYSG NKTSGVKLYT VCCKGPTGDE TAFTTLDELK
     QEHQNRHHTS AVSTKGYAQY NLFCSLTEES LMFESQSSVI ASLTWNHVEK MLECPPLSSK
     ATLNIKVAVG DIRSPLYQTY REMEFLLALA GGLRTGEIEW LEPVETQSAV DLTRALIEEL
     ENLAHGVPGH SAKASEKQKA KPDAAFSSMV IERGDLDFTE QLWEKMRKSV TSYQDITECM
     KIVVKAVKLG KIKPWIHKDS NSTLSKLILQ SYQQQIDSVP LTGLAPANML LELGLDKIRK
     DFINYLVGKE LTTLNYLSYY LDTEVDLQEQ VVRVRKLHHL LEILGTCSTF LSLPHERLFL
     FTQSCLQYYK TSNYDEDHIF QLQIKPALIS YFYEKEQPFS WAVEVSSGQG SKEVKTSLYL
     SDKPLVDHIH LDLDVSLNES VNGDSEKMFY YRTMVSCSLI NFI