ZWILC_DROME
ID ZWILC_DROME Reviewed; 641 AA.
AC Q9VA00; Q95TU6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein zwilch;
GN Name=Zwilch {ECO:0000312|FlyBase:FBgn0061476};
GN ORFNames=CG18729 {ECO:0000312|FlyBase:FBgn0061476};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-641.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE RZZ
RP COMPLEX, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=12686595; DOI=10.1091/mbc.e02-09-0624;
RA Williams B.C., Li Z., Liu S., Williams E.V., Leung G., Yen T.J.,
RA Goldberg M.L.;
RT "Zwilch, a new component of the ZW10/ROD complex required for kinetochore
RT functions.";
RL Mol. Biol. Cell 14:1379-1391(2003).
RN [5]
RP FUNCTION.
RX PubMed=15886105; DOI=10.1016/j.cub.2005.03.052;
RA Buffin E., Lefebvre C., Huang J., Gagou M.E., Karess R.E.;
RT "Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex.";
RL Curr. Biol. 15:856-861(2005).
RN [6]
RP FUNCTION.
RX PubMed=17576797; DOI=10.1083/jcb.200702062;
RA Griffis E.R., Stuurman N., Vale R.D.;
RT "Spindly, a novel protein essential for silencing the spindle assembly
RT checkpoint, recruits dynein to the kinetochore.";
RL J. Cell Biol. 177:1005-1015(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=22685323; DOI=10.1242/jcs.099820;
RA Wainman A., Giansanti M.G., Goldberg M.L., Gatti M.;
RT "The Drosophila RZZ complex - roles in membrane trafficking and
RT cytokinesis.";
RL J. Cell Sci. 125:4014-4025(2012).
CC -!- FUNCTION: Essential component of the mitotic checkpoint, which prevents
CC cells from prematurely exiting mitosis (PubMed:15886105,
CC PubMed:17576797). Required for the assembly of the dynein-dynactin,
CC Mad2 complexes and spindly/CG15415 onto kinetochores (PubMed:15886105,
CC PubMed:17576797). Its function related to the spindle assembly
CC machinery is proposed to depend on its association in the RZZ complex
CC (PubMed:22685323). Failure to assemble the complex due to the absence
CC of any one of its components, results in the incorrect redistribution
CC of the remaining components to diverse membrane compartments
CC (PubMed:22685323). {ECO:0000269|PubMed:12686595,
CC ECO:0000269|PubMed:15886105, ECO:0000269|PubMed:17576797}.
CC -!- SUBUNIT: Component of the RZZ complex composed of rod, Zw10 and Zwilch.
CC {ECO:0000269|PubMed:12686595}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22685323}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12686595,
CC ECO:0000269|PubMed:22685323}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:22685323}. Note=Dynamic pattern of localization
CC during the cell cycle (PubMed:22685323). In most cells at interphase,
CC present diffusely in the cytoplasm (PubMed:22685323). At metaphase,
CC detected at the kinetochores and kinetochore microtubules, and in late
CC anaphase and telophase accumulates at the spindle envelope midzone
CC (PubMed:12686595, PubMed:22685323). {ECO:0000269|PubMed:12686595,
CC ECO:0000269|PubMed:22685323}.
CC -!- DISRUPTION PHENOTYPE: Lagging chromosomes at anaphase and precocious
CC sister chromatid separation upon activation of the spindle checkpoint
CC (PubMed:12686595). In spermatocytes the number of Golgi structures are
CC not affected but they show a mild defect in shape (PubMed:22685323).
CC {ECO:0000269|PubMed:12686595, ECO:0000269|PubMed:22685323}.
CC -!- SIMILARITY: Belongs to the ZWILCH family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL13732.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF57128.1; -; Genomic_DNA.
DR EMBL; AY058503; AAL13732.1; ALT_INIT; mRNA.
DR RefSeq; NP_733421.1; NM_170542.2.
DR AlphaFoldDB; Q9VA00; -.
DR BioGRID; 72854; 6.
DR IntAct; Q9VA00; 1.
DR STRING; 7227.FBpp0085138; -.
DR iPTMnet; Q9VA00; -.
DR PaxDb; Q9VA00; -.
DR PRIDE; Q9VA00; -.
DR EnsemblMetazoa; FBtr0085777; FBpp0085138; FBgn0061476.
DR GeneID; 59247; -.
DR KEGG; dme:Dmel_CG18729; -.
DR UCSC; CG18729-RA; d. melanogaster.
DR CTD; 55055; -.
DR FlyBase; FBgn0061476; Zwilch.
DR VEuPathDB; VectorBase:FBgn0061476; -.
DR eggNOG; KOG4803; Eukaryota.
DR GeneTree; ENSGT00390000013696; -.
DR HOGENOM; CLU_029219_0_0_1; -.
DR InParanoid; Q9VA00; -.
DR OMA; EFIFTES; -.
DR OrthoDB; 1451737at2759; -.
DR PhylomeDB; Q9VA00; -.
DR BioGRID-ORCS; 59247; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 59247; -.
DR PRO; PR:Q9VA00; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0061476; Expressed in cleaving embryo and 17 other tissues.
DR ExpressionAtlas; Q9VA00; baseline and differential.
DR Genevisible; Q9VA00; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:FlyBase.
DR GO; GO:1990423; C:RZZ complex; IDA:FlyBase.
DR GO; GO:0051233; C:spindle midzone; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IMP:FlyBase.
DR GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR InterPro; IPR018630; Zwilch.
DR PANTHER; PTHR15995; PTHR15995; 1.
DR Pfam; PF09817; Zwilch; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW Kinetochore; Mitosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..641
FT /note="Protein zwilch"
FT /id="PRO_0000314808"
FT MOD_RES 312
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
SQ SEQUENCE 641 AA; 72442 MW; EF91716359503EC9 CRC64;
MSASANLANV YAELMRRCGE SYTITYGAPP TYLVSMVGAA EAGKKIVLVF KEDRNGAVAR
LRTTPTRAAP KKEGSADLDL TGSPLKDDCL VDAIADLSID LQLDHSNPWK LEEEYQRGIP
VDKARSIVCS EFLQLAEGLG SVWFLCDGSD LGQTQLLQYE FNPTHFSRGI LSYQGVRPAY
LVTSQALVRH HGKTPDETLI ENSYQVNPHM RLRCSWTSSA ALPLLVNLND CDVALNHTFR
VGDCGPLTQD FMNQLRILVY IREDIVSYHT DVKQGVSRDP TYRCGSGIDM DELRESINQT
MTDVSGLIGR YSISNAEFDI EDVVQRAKVR QLTDLTDKLW ELLKCCHSYK DLKIAFSMLF
QCAARCNIVN TPTNKNRLAK IITELANRRL AMPCLSGAEP LELLLEIGLE KLYKDYEFIY
TESKMCSTNL LKDDSSGASM DDGSPQNLPQ LRKSLHNAVR GDPTPGAGMR KTLLHHHGAV
NSRSTKYAGS DDDAGFKNSH FDEHESTERI SKLFQIHCTL EHLLMMHIHL NLANVYNDVC
SELLKKPPKL VESIDDQLSD VMDIRLSAHY VRDHLDGKDP YSRHITMRSH NKFRELKTTF
YFNSENICPP NLAQCFQCDD KEMVKERTYH SWIYHKIRSL K
