ZWINT_MOUSE
ID ZWINT_MOUSE Reviewed; 252 AA.
AC Q9CQU5; Q3UYT8; Q91VI4; Q9D0W9;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=ZW10 interactor;
DE AltName: Full=ZW10-interacting protein 1;
DE Short=Zwint-1;
GN Name=Zwint; Synonyms=D10Ertd749e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Hippocampus, Small intestine, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland, and Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Part of the MIS12 complex, which is required for kinetochore
CC formation and spindle checkpoint activity. Required to target ZW10 to
CC the kinetochore at prometaphase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ZW10 and MIS12. Interacts with the NDC80
CC subunit of the NDC80 complex specifically during mitosis. Also
CC interacts with KNL1, CETN3, DSN1 and PMF1 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC kinetochore {ECO:0000250}. Note=Localizes to kinetochores from late
CC prophase to anaphase. {ECO:0000250}.
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DR EMBL; AK004305; BAB23256.1; -; mRNA.
DR EMBL; AK008144; BAB25492.1; -; mRNA.
DR EMBL; AK013556; BAB28904.1; -; mRNA.
DR EMBL; AK075977; BAC36090.1; -; mRNA.
DR EMBL; AK134380; BAE22123.1; -; mRNA.
DR EMBL; BC013559; AAH13559.1; -; mRNA.
DR EMBL; BC027100; AAH27100.1; -; mRNA.
DR EMBL; BC034870; AAH34870.1; -; mRNA.
DR CCDS; CCDS23920.1; -.
DR RefSeq; NP_001280612.1; NM_001293683.1.
DR RefSeq; NP_001280613.1; NM_001293684.1.
DR RefSeq; NP_079911.1; NM_025635.4.
DR AlphaFoldDB; Q9CQU5; -.
DR SMR; Q9CQU5; -.
DR BioGRID; 206739; 27.
DR ComplexPortal; CPX-5702; Kinetochore KNL1 complex.
DR IntAct; Q9CQU5; 22.
DR STRING; 10090.ENSMUSP00000101071; -.
DR iPTMnet; Q9CQU5; -.
DR PhosphoSitePlus; Q9CQU5; -.
DR SwissPalm; Q9CQU5; -.
DR EPD; Q9CQU5; -.
DR MaxQB; Q9CQU5; -.
DR PaxDb; Q9CQU5; -.
DR PeptideAtlas; Q9CQU5; -.
DR PRIDE; Q9CQU5; -.
DR ProteomicsDB; 302155; -.
DR Antibodypedia; 28018; 273 antibodies from 31 providers.
DR DNASU; 52696; -.
DR Ensembl; ENSMUST00000020081; ENSMUSP00000020081; ENSMUSG00000019923.
DR Ensembl; ENSMUST00000105431; ENSMUSP00000101071; ENSMUSG00000019923.
DR Ensembl; ENSMUST00000160337; ENSMUSP00000124429; ENSMUSG00000019923.
DR GeneID; 52696; -.
DR KEGG; mmu:52696; -.
DR UCSC; uc007foy.2; mouse.
DR CTD; 11130; -.
DR MGI; MGI:1289227; Zwint.
DR VEuPathDB; HostDB:ENSMUSG00000019923; -.
DR eggNOG; ENOG502S6PG; Eukaryota.
DR GeneTree; ENSGT00390000017639; -.
DR HOGENOM; CLU_089675_0_0_1; -.
DR InParanoid; Q9CQU5; -.
DR OMA; QSIGDTM; -.
DR OrthoDB; 1313012at2759; -.
DR PhylomeDB; Q9CQU5; -.
DR TreeFam; TF338101; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 52696; 21 hits in 74 CRISPR screens.
DR ChiTaRS; Zwint; mouse.
DR PRO; PR:Q9CQU5; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q9CQU5; protein.
DR Bgee; ENSMUSG00000019923; Expressed in ventromedial nucleus of hypothalamus and 274 other tissues.
DR ExpressionAtlas; Q9CQU5; baseline and differential.
DR Genevisible; Q9CQU5; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0000776; C:kinetochore; ISO:MGI.
DR GO; GO:0043005; C:neuron projection; ISO:MGI.
DR GO; GO:0031617; C:NMS complex; IC:ComplexPortal.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:MGI.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051649; P:establishment of localization in cell; ISO:MGI.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; ISO:MGI.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISO:MGI.
DR InterPro; IPR029092; Zwint-1.
DR PANTHER; PTHR31504; PTHR31504; 1.
DR Pfam; PF15556; Zwint; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..252
FT /note="ZW10 interactor"
FT /id="PRO_0000066595"
FT REGION 80..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 86..161
FT /note="Interaction with NDC80 and ZW10"
FT /evidence="ECO:0000250"
FT COILED 120..221
FT /evidence="ECO:0000255"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 249
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VIL3"
FT CONFLICT 1..7
FT /note="MADAEKN -> PTRP (in Ref. 2; AAH13559)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 252 AA; 28713 MW; 56CF28F7493CD4E8 CRC64;
MADAEKNAVA EKNNAVATKE VLAEAAAILE PVGLQEEAEL PAKIMEEFMR NSRKKDKLLC
SQLQVVNFLQ TFLAQEDTEQ SPDALASEDA SRQKATETKE QWKDMKATYM DHVDVIKCAL
SEALPQVKEA HRKYTELQKA FEQLEAKKRV LEEKLQLAQK QWVLQQKRLQ NLTKISAEVK
RRRKRALEKL DGSHQELETL KQQAGQEQEK LQRNQSYLQL LCSLQNKLVI SEGKAEDKDV
KGRALTAKSK SP
