ZWINT_RAT
ID ZWINT_RAT Reviewed; 266 AA.
AC Q8VIL3; Q546Y5;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=ZW10 interactor;
DE AltName: Full=SNAP25-interacting protein 30;
DE AltName: Full=Scoilin;
DE AltName: Full=ZW10-interacting protein 1;
DE Short=Zwint-1;
GN Name=Zwint; Synonyms=Sip30;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH SNAP25.
RC STRAIN=Sprague-Dawley;
RX PubMed=12068081; DOI=10.1046/j.1471-4159.2002.00937.x;
RA Lee H.-K., Safieddine S., Petralia R.S., Wenthold R.J.;
RT "Identification of a novel SNAP25 interacting protein (SIP30).";
RL J. Neurochem. 81:1338-1347(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Okamoto M., Suedhof T.C.;
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pituitary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 13-30; 123-133 AND 156-163, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Hippocampus;
RA Lubec G., Chen W.-Q.;
RL Submitted (APR-2007) to UniProtKB.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-265, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Part of the MIS12 complex, which is required for kinetochore
CC formation and spindle checkpoint activity. Required to target ZW10 to
CC the kinetochore at prometaphase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ZW10 and MIS12. Interacts with the NDC80
CC subunit of the NDC80 complex specifically during mitosis. Also
CC interacts with KNL1, CETN3, DSN1 and PMF1 (By similarity). Interacts
CC with SNAP25. {ECO:0000250, ECO:0000269|PubMed:12068081}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC kinetochore {ECO:0000250}. Note=Localizes to kinetochores from late
CC prophase to anaphase. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed abundantly in brain and at lower levels
CC in testis and kidney.
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DR EMBL; AF439397; AAL35221.1; -; mRNA.
DR EMBL; AF532776; AAM95974.1; -; mRNA.
DR EMBL; BC063144; AAH63144.1; -; mRNA.
DR RefSeq; NP_671479.1; NM_147138.2.
DR AlphaFoldDB; Q8VIL3; -.
DR SMR; Q8VIL3; -.
DR BioGRID; 251696; 4.
DR STRING; 10116.ENSRNOP00000067414; -.
DR iPTMnet; Q8VIL3; -.
DR PhosphoSitePlus; Q8VIL3; -.
DR jPOST; Q8VIL3; -.
DR PaxDb; Q8VIL3; -.
DR PRIDE; Q8VIL3; -.
DR Ensembl; ENSRNOT00000074404; ENSRNOP00000067414; ENSRNOG00000048682.
DR GeneID; 257644; -.
DR KEGG; rno:257644; -.
DR CTD; 11130; -.
DR RGD; 628671; Zwint.
DR eggNOG; ENOG502S6PG; Eukaryota.
DR GeneTree; ENSGT00390000017639; -.
DR HOGENOM; CLU_089675_0_0_1; -.
DR InParanoid; Q8VIL3; -.
DR OMA; QSIGDTM; -.
DR OrthoDB; 1313012at2759; -.
DR PhylomeDB; Q8VIL3; -.
DR Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR Reactome; R-RNO-68877; Mitotic Prometaphase.
DR Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR PRO; PR:Q8VIL3; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000048682; Expressed in Ammon's horn and 20 other tissues.
DR Genevisible; Q8VIL3; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IDA:RGD.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:RGD.
DR GO; GO:0000776; C:kinetochore; ISO:RGD.
DR GO; GO:0043005; C:neuron projection; IDA:RGD.
DR GO; GO:0016604; C:nuclear body; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:RGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051649; P:establishment of localization in cell; ISO:RGD.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; ISO:RGD.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISO:RGD.
DR InterPro; IPR029092; Zwint-1.
DR PANTHER; PTHR31504; PTHR31504; 1.
DR Pfam; PF15556; Zwint; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW Direct protein sequencing; Kinetochore; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..266
FT /note="ZW10 interactor"
FT /id="PRO_0000066596"
FT REGION 86..161
FT /note="Interaction with NDC80 and ZW10"
FT /evidence="ECO:0000250"
FT REGION 95..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 136..237
FT /evidence="ECO:0000255"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 265
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 266 AA; 30142 MW; 869E69510E35CC6C CRC64;
MADAEKNAVA EKNAVAEENA VAEENAVADK NATKEVLAEA ASVLEPVGLP EEAELPAKIM
EEFMRNSRKK DKLLCSQLQV VNFLQTFLAQ EDNTDQNPDA LASEDTSRQK ATETKEQWKE
LKATYMDHVD VIKCALSEAL PQVKEAHRKY TELQKAFEQL EAKKRVLEEK LQLAQKQWVL
QQKRLQNLTK ISAEVKRRRK RALEKLDGSH QELETLKQQA GQEQEKLQRN QSYLQLLCSL
QNKLVISESK ADDKDVKGPA LPPKSP
