CCA_FRANO
ID CCA_FRANO Reviewed; 84 AA.
AC Q47907;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=CCA tRNA nucleotidyltransferase;
DE EC=2.7.7.72;
DE AltName: Full=CCA-adding enzyme;
DE AltName: Full=tRNA CCA-pyrophosphorylase;
DE AltName: Full=tRNA adenylyltransferase;
DE Flags: Fragment;
GN Name=cca;
OS Francisella novicida.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=264;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=U112;
RX PubMed=7881549; DOI=10.1099/13500872-140-12-3309;
RA Mdluli K.E., Anthony L.S., Baron G.S., McDonald M.K., Myltseva S.V.,
RA Nano F.E.;
RT "Serum-sensitive mutation of Francisella novicida: association with an ABC
RT transporter gene.";
RL Microbiology 140:3309-3318(1994).
CC -!- FUNCTION: This enzyme carries out synthesis of the tRNA CCA terminus
CC without the direction of a template using the multiple accepting and
CC donating subsites within its active site. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3
CC diphosphate; Xref=Rhea:RHEA:14433, Rhea:RHEA-COMP:10465, Rhea:RHEA-
CC COMP:10468, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:83071; EC=2.7.7.72;
CC -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC polymerase family. {ECO:0000305}.
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DR EMBL; L17003; AAD15236.1; -; Genomic_DNA.
DR AlphaFoldDB; Q47907; -.
DR SMR; Q47907; -.
DR STRING; 676032.FN3523_1671; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0052929; F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0052928; F:CTP:3'-cytidine-tRNA cytidylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0052927; F:CTP:tRNA cytidylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016437; F:tRNA cytidylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.460.10; -; 1.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002646; PolA_pol_head_dom.
DR Pfam; PF01743; PolyA_pol; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
PE 3: Inferred from homology;
KW ATP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Nucleotidyltransferase; RNA-binding; Transferase; tRNA processing.
FT CHAIN 1..>84
FT /note="CCA tRNA nucleotidyltransferase"
FT /id="PRO_0000139088"
FT BINDING 8
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT BINDING 8
FT /ligand="CTP"
FT /ligand_id="ChEBI:CHEBI:37563"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT BINDING 11
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT BINDING 11
FT /ligand="CTP"
FT /ligand_id="ChEBI:CHEBI:37563"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT BINDING 21
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT BINDING 23
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q7SIB1"
FT NON_TER 84
SQ SEQUENCE 84 AA; 9527 MW; 2C8625AF99C84095 CRC64;
MKFYLVGGAV RDMLLGITPK DKDWVVVGAT EDEMLANGFI KIAANFPVFI HPQTKQEYAL
ARSEKKTRNG YHGFEVNFSK YITL
