ID   CCB23_FAXLI             Reviewed;         202 AA.
AC   P80363;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Crustacean calcium-binding protein 23;
DE            Short=CCBP-23;
OS   Faxonius limosus (Spinycheek crayfish) (Orconectes limosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea;
OC   Astacoidea; Cambaridae; Faxonius.
OX   NCBI_TaxID=28379;
RN   [1]
RP   PROTEIN SEQUENCE, AND ACETYLATION AT SER-1.
RC   TISSUE=Abdominal muscle;
RX   PubMed=7851448; DOI=10.1111/j.1432-1033.1995.tb20363.x;
RA   Sauter A., Staudenmann W., Hughes G.J., Heizmann C.W.;
RT   "A novel EF-hand Ca(2+)-binding protein from abdominal muscle of
RT   crustaceans with similarity to calcyphosine from dog thyroidea.";
RL   Eur. J. Biochem. 227:97-101(1995).
CC   -!- FUNCTION: Possibly acts as a regulatory protein and not as a calcium
CC       buffer or transport protein.
CC   -!- SUBUNIT: Monomer or disulfide-linked dimers.
CC   -!- TISSUE SPECIFICITY: Striated muscle and brain.
CC   -!- MISCELLANEOUS: This protein binds calcium.
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DR   PIR; S67524; S67524.
DR   iPTMnet; P80363; -.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13202; EF-hand_5; 1.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 4.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 4.
PE   1: Evidence at protein level;
KW   Acetylation; Calcium; Direct protein sequencing; Disulfide bond;
KW   Metal-binding; Repeat.
FT   CHAIN           1..202
FT                   /note="Crustacean calcium-binding protein 23"
FT                   /id="PRO_0000073544"
FT   DOMAIN          33..68
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          69..104
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          105..140
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          148..185
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         84
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         93
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         118
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         122
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         129
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   MOD_RES         1
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000269|PubMed:7851448"
FT   UNSURE          35
FT                   /note="L or I"
FT   UNSURE          36
FT                   /note="L or I"
SQ   SEQUENCE   202 AA;  22850 MW;  191400E4BA8DADDD CRC64;
     SEAKLKEAAK ETLKTTTDPL EKLRSHCLSQ GYSGLLSLGR LFRRLDKDRS WTLSKEELSR
     GVGQFGLDLS DGDINKLFSS FEKDGQSGIN YEEFLESLRP EMTEPRKKAV EAAFKHLDKT
     GDGVVGLEDI KGKYSAKTHP KVVKGEATED EILKKFLNMF ETNTSVDGKV TKKEFFDYYS
     GLSKAIDEDE YFVSMVNIMW XX