CCB2_ARATH
ID CCB2_ARATH Reviewed; 275 AA.
AC Q9FJ81; Q67YA4;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB2, chloroplastic {ECO:0000305};
DE AltName: Full=Protein HIGH CHLOROPHYLL FLUORESCENCE 208 {ECO:0000303|PubMed:17971335};
DE Flags: Precursor;
GN Name=CCB2 {ECO:0000303|PubMed:18593701};
GN Synonyms=HCF208 {ECO:0000303|PubMed:17971335};
GN OrderedLocusNames=At5g52110 {ECO:0000312|Araport:AT5G52110};
GN ORFNames=MSG15.21 {ECO:0000312|EMBL:BAB11059.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17971335; DOI=10.1093/pcp/pcm146;
RA Lyska D., Paradies S., Meierhoff K., Westhoff P.;
RT "HCF208, a homolog of Chlamydomonas CCB2, is required for accumulation of
RT native cytochrome b6 in Arabidopsis thaliana.";
RL Plant Cell Physiol. 48:1737-1746(2007).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND DISRUPTION PHENOTYPE.
RX PubMed=18593701; DOI=10.1074/jbc.m803869200;
RA Lezhneva L., Kuras R., Ephritikhine G., de Vitry C.;
RT "A novel pathway of cytochrome c biogenesis is involved in the assembly of
RT the cytochrome b6f complex in arabidopsis chloroplasts.";
RL J. Biol. Chem. 283:24608-24616(2008).
CC -!- FUNCTION: Required for the biogenesis and accumulation of native
CC cytochrome b6 in the thylakoid membrane. Controls the conversion of
CC apocytochrome b6 to holocytochrome b6. Required for covalent binding of
CC the c-type heme to cytochrome b6. {ECO:0000269|PubMed:17971335,
CC ECO:0000269|PubMed:18593701}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305|PubMed:18593701}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: High chlorophyll fluorescence phenotype due to an
CC impaired photosynthetic electron flow (PubMed:17971335). Seedling
CC lethal when grown on soil. On agar plates supplied with sucrose,
CC seedlings grow very slowly with a chlorotic phenotype. Deficiency in
CC the accumulation of the subunits of the cytochrome b6f complex and lack
CC of covalent heme binding to cytochrome b6 (PubMed:17971335,
CC PubMed:18593701). {ECO:0000269|PubMed:17971335,
CC ECO:0000269|PubMed:18593701}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD43126.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD44327.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB015478; BAB11059.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96172.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96173.1; -; Genomic_DNA.
DR EMBL; BT029468; ABK59697.1; -; mRNA.
DR EMBL; AK175363; BAD43126.1; ALT_FRAME; mRNA.
DR EMBL; AK176564; BAD44327.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001032060.1; NM_001036983.3.
DR RefSeq; NP_200024.1; NM_124590.2.
DR AlphaFoldDB; Q9FJ81; -.
DR STRING; 3702.AT5G52110.2; -.
DR PaxDb; Q9FJ81; -.
DR PRIDE; Q9FJ81; -.
DR ProteomicsDB; 223955; -.
DR EnsemblPlants; AT5G52110.1; AT5G52110.1; AT5G52110.
DR EnsemblPlants; AT5G52110.2; AT5G52110.2; AT5G52110.
DR GeneID; 835287; -.
DR Gramene; AT5G52110.1; AT5G52110.1; AT5G52110.
DR Gramene; AT5G52110.2; AT5G52110.2; AT5G52110.
DR KEGG; ath:AT5G52110; -.
DR Araport; AT5G52110; -.
DR TAIR; locus:2173048; AT5G52110.
DR eggNOG; ENOG502QQM6; Eukaryota.
DR HOGENOM; CLU_079216_0_0_1; -.
DR InParanoid; Q9FJ81; -.
DR OrthoDB; 1076342at2759; -.
DR PhylomeDB; Q9FJ81; -.
DR PRO; PR:Q9FJ81; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ81; baseline and differential.
DR Genevisible; Q9FJ81; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010190; P:cytochrome b6f complex assembly; IMP:TAIR.
DR InterPro; IPR044970; CCB2.
DR InterPro; IPR021325; CCB2/CCB4.
DR PANTHER; PTHR36403; PTHR36403; 1.
DR Pfam; PF11152; CCB2_CCB4; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..19
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 20..275
FT /note="Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B
FT CCB2, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000433263"
FT TOPO_DOM 20..65
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:18593701"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..93
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:18593701"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..275
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:18593701"
SQ SEQUENCE 275 AA; 30607 MW; 53BB8AE8C930086B CRC64;
MSIQICNFPF HPKFALQPRA QRSTRIFART ENDSPQSKTS DQQLNLSVLR FTFGIPGFDE
SYLPRWIGYG FGSLLLLNHF SASAPISESQ MRSEALGLSL AAFSIALPYI GKFLKGSVVE
QRSLPEEGEQ VFVISSNIGD SLKEDLAWAT YVLLRNTSTI AVLISVQGEL CVRGYWNCPD
QMSKAQLHDW FKKKVDEIGL ADVKETLYFP QYAGSALSLD ILPDGTRSLF VQPLVQNTNE
PQKVNGFLLV ASTAGYAYSD KDRAWIGAMA EKFRG
