CCB4_ARATH
ID CCB4_ARATH Reviewed; 297 AA.
AC Q6NQK9; Q9XIE5;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=CCB4 {ECO:0000303|PubMed:18593701};
GN OrderedLocusNames=At1g59840 {ECO:0000312|Araport:AT1G59840};
GN ORFNames=F23H11.16 {ECO:0000312|EMBL:AAD39336.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND DISRUPTION PHENOTYPE.
RX PubMed=18593701; DOI=10.1074/jbc.m803869200;
RA Lezhneva L., Kuras R., Ephritikhine G., de Vitry C.;
RT "A novel pathway of cytochrome c biogenesis is involved in the assembly of
RT the cytochrome b6f complex in arabidopsis chloroplasts.";
RL J. Biol. Chem. 283:24608-24616(2008).
CC -!- FUNCTION: Required for the biogenesis and accumulation of native
CC cytochrome b6 in the thylakoid membrane. Controls the conversion of
CC apocytochrome b6 to holocytochrome b6. Required for covalent binding of
CC the c-type heme to cytochrome b6. {ECO:0000269|PubMed:18593701}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305|PubMed:18593701}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethal when grown on soil. On agar
CC plates supplied with sucrose, seedlings grow very slowly with a
CC chlorotic phenotype. Deficiency in the accumulation of the subunits of
CC the cytochrome b6f complex and lack of covalent heme binding to
CC cytochrome b6. {ECO:0000269|PubMed:18593701}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD39336.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007258; AAD39336.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33626.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33627.1; -; Genomic_DNA.
DR EMBL; BT010443; AAQ62444.1; -; mRNA.
DR EMBL; AK175262; BAD43025.1; -; mRNA.
DR EMBL; AK175368; BAD43131.1; -; mRNA.
DR EMBL; AK221539; BAD94887.1; -; mRNA.
DR PIR; E96622; E96622.
DR RefSeq; NP_176193.2; NM_104677.5.
DR RefSeq; NP_974051.1; NM_202322.2.
DR AlphaFoldDB; Q6NQK9; -.
DR IntAct; Q6NQK9; 1.
DR STRING; 3702.AT1G59840.2; -.
DR PaxDb; Q6NQK9; -.
DR PRIDE; Q6NQK9; -.
DR ProteomicsDB; 222805; -.
DR EnsemblPlants; AT1G59840.1; AT1G59840.1; AT1G59840.
DR EnsemblPlants; AT1G59840.2; AT1G59840.2; AT1G59840.
DR GeneID; 842278; -.
DR Gramene; AT1G59840.1; AT1G59840.1; AT1G59840.
DR Gramene; AT1G59840.2; AT1G59840.2; AT1G59840.
DR KEGG; ath:AT1G59840; -.
DR Araport; AT1G59840; -.
DR TAIR; locus:2025896; AT1G59840.
DR eggNOG; ENOG502QSXP; Eukaryota.
DR HOGENOM; CLU_082867_1_0_1; -.
DR InParanoid; Q6NQK9; -.
DR OMA; RSYTKQD; -.
DR OrthoDB; 1516649at2759; -.
DR PhylomeDB; Q6NQK9; -.
DR PRO; PR:Q6NQK9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NQK9; baseline and differential.
DR Genevisible; Q6NQK9; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010190; P:cytochrome b6f complex assembly; IMP:TAIR.
DR InterPro; IPR021325; CCB2/CCB4.
DR InterPro; IPR044705; CCB4.
DR PANTHER; PTHR34943; PTHR34943; 1.
DR Pfam; PF11152; CCB2_CCB4; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..33
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 34..297
FT /note="Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B
FT CCB4, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000433264"
FT TOPO_DOM 34..70
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:18593701"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..103
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:18593701"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..297
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:18593701"
SQ SEQUENCE 297 AA; 32222 MW; E07455D51B19E5A7 CRC64;
MEARIILLRI QIPWSANRQF SHPPLDFPRF IRASSSSTSQ KPKTYEGPKP RKNLVADFIS
KNDDLVRSLP IYVGGASLLA VLFNRTVSGI APVADASSSQ SRADLLALGL AVTNLLTGLV
WLSIRPKSIT PVNPKGVECK VVESDLPASM VSELLWAWES LKVATCCKSL VIVYNGICLI
QIGMVAESPE DKKTVIVKTD KLMQGSVYRG VMKSKAQSYL ANLSLYPGRS ELPFLPANTQ
AVILQPLGDK GIAVIGGNTI RGFTSSDQAW ISSIGEKLDA TLGRYFVDSD EISRVTV
