CCC2_ORYSJ
ID CCC2_ORYSJ Reviewed; 994 AA.
AC Q657W3; A4VCJ9; Q0JNC7; Q657W2;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Cation-chloride cotransporter 2;
DE Short=OsCCC2;
DE AltName: Full=Potassium-chloride cotransporter 2;
GN Name=CCC2; Synonyms=KCC2; OrderedLocusNames=Os01g0304100, LOC_Os01g19850;
GN ORFNames=P0475H04.13, P0475H04.14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP IDENTIFICATION.
RX PubMed=17355435; DOI=10.1111/j.1365-313x.2007.03048.x;
RA Colmenero-Flores J.M., Martinez G., Gamba G., Vazquez N., Iglesias D.J.,
RA Brumos J., Talon M.;
RT "Identification and functional characterization of cation-chloride
RT cotransporters in plants.";
RL Plant J. 50:278-292(2007).
CC -!- FUNCTION: Probable cation/chloride cotransporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD44902.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD44903.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF04751.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP002871; BAD44902.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP002871; BAD44903.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAF04751.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BN000835; CAJ40614.1; -; mRNA.
DR RefSeq; XP_015632062.1; XM_015776576.1.
DR AlphaFoldDB; Q657W3; -.
DR SMR; Q657W3; -.
DR STRING; 4530.OS01T0304100-00; -.
DR PaxDb; Q657W3; -.
DR PRIDE; Q657W3; -.
DR GeneID; 4323905; -.
DR KEGG; osa:4323905; -.
DR eggNOG; KOG2082; Eukaryota.
DR InParanoid; Q657W3; -.
DR OrthoDB; 349744at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IBA:GO_Central.
DR GO; GO:0006884; P:cell volume homeostasis; IBA:GO_Central.
DR GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR PANTHER; PTHR11827; PTHR11827; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF03522; SLC12; 2.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..994
FT /note="Cation-chloride cotransporter 2"
FT /id="PRO_0000410468"
FT TOPO_DOM 1..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..174
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..287
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..372
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..412
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 434..448
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 470..505
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 527..529
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 553..558
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 580..585
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 586..606
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 607..994
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 268
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 274
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 337
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 370
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 994 AA; 109326 MW; 25B35BD1DA90CE60 CRC64;
MERGGFGGAG RHDEEAPAMR PAPQQRYRTV ESHDRAVVQM APMEFGSSAD ASASAGPRYI
KPGTNLRTDA RMHMASSNGR SSNGSQGDSK LELFGFDSLV NILGLKRMVG EQAQASASTR
DGENAGIAIG HPKETETKLD TMMGVFVPCL QNILGIIYYI RFTWIVGMGG VWQSLVLVAF
CGSCTFLTTI SLSAIATNGA MKGGGPYYLI GRALGPEVGV SIGLCFFLGN AVAGAMYVLG
AVETFLDAVP SAEFFQESVT VVTNTFVNGT AAGNATTIST PNLHDLQVYG IIVTILLCFI
VFGGVKIINK VAPAFLIPVL FSILCIYIGV FIAPRPNASK WITGLSITTL KDNWSSDYQR
TNNAGVPDPN GSIYWDFNAL LGLYFPAVTG IMAGSNRSAS LKDTQRSIPI GTLHATISTT
MMYLLSVFLF GALSTREGLL TDRLLCAAVA WPSPAVVYAG IILSTLGAAL QSLTGAPRLL
AAIANDDILP VLNYFKAYEG SEPHVATLFT SFICISCVII GNLDVITPTI TMFFLLCYAG
VNLSCFLLDL LDAPSWRPRW KLHHWSLSLI GALLCIVIMF MISWTFTVVS LALASLIYYY
VSLKGKAGDW GDGFKSAYFQ LALRSLRSMG ANQVHPKNWY PIPLIFCRPW GKLPEDVPCH
PKLADFANCM KKKGRGMSIF VSIIDGDYHE SAEDAKTACR QLSAYIDYRR CEGVAEIIVA
PSTSIGFRSI VQTMGLGNLK PNIVVMRYPE IWRRENLTQI PSTFVSIIND CITANKAVVI
VKGLDEWPNE YQRQYGTIDL YWIVRDGGLM LLLSQLLLTK ESFESCKIQV FCIAEEDTEA
EELKADVKKF LYDLRMQADV IVVTVKSWEA DPDRSGGSKK DDPEVYRSAQ SRIRTYISQL
KEAAERERRP LMEGGKQVVV DEQKVEKFLY TMLKLNATIL RHSRMAVVVL VSLPPPPLNH
LAYCYMEYMD LLVENIPRIL IVRGYRRDVV TLFT
