CCCP_DROME
ID CCCP_DROME Reviewed; 260 AA.
AC O76879; Q24484; Q8MM43; Q8MM44; Q8SY82; Q9W4W9;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Circadian clock-controlled protein daywake {ECO:0000303|PubMed:31080079};
DE Flags: Precursor;
GN Name=dyw {ECO:0000303|PubMed:31080079, ECO:0000312|FlyBase:FBgn0000092};
GN Synonyms=anon-3B1.2 {ECO:0000312|FlyBase:FBgn0000092};
GN ORFNames=CG2650 {ECO:0000312|FlyBase:FBgn0000092};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF45805.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=2517256; DOI=10.1242/dev.107.4.869;
RA Lorenz L.J., Hall J.C., Rosbash M.;
RT "Expression of a Drosophila mRNA is under circadian clock control during
RT pupation.";
RL Development 107:869-880(1989).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE OF 159-260, AND VARIANT LEU-219.
RC STRAIN=AF1, AF2, AF3, AF4, K1, K11, K13, K14, K16, K17, K2, K20, K21, K3,
RC K5, K7, K9, wi1, wi10, wi12, wi13, wi14, wi15, wi16, wi17, wi18, wi19, wi2,
RC wi21, wi22, wi23, wi24, wi25, wi27, wi28, wi29, wi3, wi30, wi31, wi32,
RC wi33, wi4, wi5, wi6, wi7, wi8, wi9, ZH1, ZH13, ZH16, ZH18, ZH19, ZH2, ZH20,
RC ZH21, ZH23, ZH25, ZH29, ZH31, ZH33, ZH35, and ZH36;
RX PubMed=11975710; DOI=10.1046/j.1365-294x.2002.01491.x;
RA Schlotterer C., Harr B.;
RT "Single nucleotide polymorphisms derived from ancestral populations show no
RT evidence for biased diversity estimates in Drosophila melanogaster.";
RL Mol. Ecol. 11:947-950(2002).
RN [7]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=31080079; DOI=10.1016/j.cub.2019.04.039;
RA Yang Y., Edery I.;
RT "Daywake, an Anti-siesta Gene Linked to a Splicing-Based Thermostat from an
RT Adjoining Clock Gene.";
RL Curr. Biol. 29:1728-1734(2019).
CC -!- FUNCTION: Component of the circadian clock or downstream effector of
CC clock function (PubMed:2517256, PubMed:31080079). Required for
CC suppressing daytime sleep (siesta) under ambient environmental
CC temperatures. Part of a heat avoidance mechanism that modulates daytime
CC sleep behavior under different environmental temperatures to minimize
CC the risk of heat exposure. Under cooler ambient temperatures,
CC suppresses daytime sleep (siesta) and thus allows for longer periods of
CC daytime activity (PubMed:31080079). {ECO:0000269|PubMed:2517256,
CC ECO:0000269|PubMed:31080079}.
CC -!- TISSUE SPECIFICITY: Epidermis of newly eclosed adults.
CC {ECO:0000269|PubMed:2517256}.
CC -!- DEVELOPMENTAL STAGE: Abundantly expressed during pupation and decreases
CC rapidly with age. Expression is under pupal (eclosion) circadian clock
CC control. {ECO:0000269|PubMed:2517256}.
CC -!- INDUCTION: Expression is under the control of a thermosensitive
CC enhancer element called dmpi8 that is encoded in an intron of the
CC close-by circadian clock gene per. At lower temperatures, splicing
CC efficiency of dmpi8 is increased resulting in an increase in the
CC expression of dyw. {ECO:0000269|PubMed:31080079}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in per-expressing cells
CC increases daytime sleep levels whereas night-time sleep levels are
CC relatively unaffected. {ECO:0000269|PubMed:31080079}.
CC -!- MISCELLANEOUS: Named 'daywake' based upon its function in daytime sleep
CC suppression. {ECO:0000303|PubMed:31080079}.
CC -!- SIMILARITY: Belongs to the TO family. {ECO:0000305}.
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DR EMBL; X76206; CAA53799.1; -; Genomic_DNA.
DR EMBL; AL024485; CAA19674.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45805.2; -; Genomic_DNA.
DR EMBL; AY071726; AAL49348.1; -; mRNA.
DR EMBL; AY047980; AAN02290.1; -; Genomic_DNA.
DR EMBL; AY047981; AAN02292.1; -; Genomic_DNA.
DR EMBL; AY047982; AAN02294.1; -; Genomic_DNA.
DR EMBL; AY047983; AAN02296.1; -; Genomic_DNA.
DR EMBL; AY047984; AAN02298.1; -; Genomic_DNA.
DR EMBL; AY047985; AAN02300.1; -; Genomic_DNA.
DR EMBL; AY047986; AAN02302.1; -; Genomic_DNA.
DR EMBL; AY047987; AAN02304.1; -; Genomic_DNA.
DR EMBL; AY047988; AAN02306.1; -; Genomic_DNA.
DR EMBL; AY047989; AAN02308.1; -; Genomic_DNA.
DR EMBL; AY047990; AAN02310.1; -; Genomic_DNA.
DR EMBL; AY047991; AAN02312.1; -; Genomic_DNA.
DR EMBL; AY047992; AAN02314.1; -; Genomic_DNA.
DR EMBL; AY047993; AAN02316.1; -; Genomic_DNA.
DR EMBL; AY047994; AAN02318.1; -; Genomic_DNA.
DR EMBL; AY047995; AAN02320.1; -; Genomic_DNA.
DR EMBL; AY047996; AAN02322.1; -; Genomic_DNA.
DR EMBL; AY047997; AAN02324.1; -; Genomic_DNA.
DR EMBL; AY047998; AAN02326.1; -; Genomic_DNA.
DR EMBL; AY047999; AAN02328.1; -; Genomic_DNA.
DR EMBL; AY048000; AAN02330.1; -; Genomic_DNA.
DR EMBL; AY048001; AAN02332.1; -; Genomic_DNA.
DR EMBL; AY048002; AAN02334.1; -; Genomic_DNA.
DR EMBL; AY048003; AAN02336.1; -; Genomic_DNA.
DR EMBL; AY048004; AAN02338.1; -; Genomic_DNA.
DR EMBL; AY048005; AAN02340.1; -; Genomic_DNA.
DR EMBL; AY048006; AAN02342.1; -; Genomic_DNA.
DR EMBL; AY048007; AAN02344.1; -; Genomic_DNA.
DR EMBL; AY048008; AAN02346.1; -; Genomic_DNA.
DR EMBL; AY048009; AAN02348.1; -; Genomic_DNA.
DR EMBL; AY048010; AAN02350.1; -; Genomic_DNA.
DR EMBL; AY048011; AAN02352.1; -; Genomic_DNA.
DR EMBL; AY048012; AAN02354.1; -; Genomic_DNA.
DR EMBL; AY048013; AAN02356.1; -; Genomic_DNA.
DR EMBL; AY048014; AAN02358.1; -; Genomic_DNA.
DR EMBL; AY048015; AAN02360.1; -; Genomic_DNA.
DR EMBL; AY048016; AAN02362.1; -; Genomic_DNA.
DR EMBL; AY048017; AAN02364.1; -; Genomic_DNA.
DR EMBL; AY048018; AAN02366.1; -; Genomic_DNA.
DR EMBL; AY048019; AAN02368.1; -; Genomic_DNA.
DR EMBL; AY048020; AAN02370.1; -; Genomic_DNA.
DR EMBL; AY048021; AAN02372.1; -; Genomic_DNA.
DR EMBL; AY048022; AAN02374.1; -; Genomic_DNA.
DR EMBL; AY048023; AAN02376.1; -; Genomic_DNA.
DR EMBL; AY048024; AAN02378.1; -; Genomic_DNA.
DR EMBL; AY048025; AAN02380.1; -; Genomic_DNA.
DR EMBL; AY048026; AAN02382.1; -; Genomic_DNA.
DR EMBL; AY048027; AAN02384.1; -; Genomic_DNA.
DR EMBL; AY048028; AAN02386.1; -; Genomic_DNA.
DR EMBL; AY048029; AAN02388.1; -; Genomic_DNA.
DR EMBL; AY048030; AAN02390.1; -; Genomic_DNA.
DR EMBL; AY048031; AAN02392.1; -; Genomic_DNA.
DR EMBL; AY048032; AAN02394.1; -; Genomic_DNA.
DR EMBL; AY048033; AAN02396.1; -; Genomic_DNA.
DR EMBL; AY048034; AAN02398.1; -; Genomic_DNA.
DR EMBL; AY048035; AAN02400.1; -; Genomic_DNA.
DR EMBL; AY048036; AAN02402.1; -; Genomic_DNA.
DR EMBL; AY048037; AAN02404.1; -; Genomic_DNA.
DR EMBL; AY048038; AAN02406.1; -; Genomic_DNA.
DR EMBL; AY048040; AAN02408.1; -; Genomic_DNA.
DR EMBL; AY048041; AAN02410.1; -; Genomic_DNA.
DR EMBL; AY048042; AAN02412.1; -; Genomic_DNA.
DR PIR; A60091; A60091.
DR RefSeq; NP_570016.1; NM_130660.2.
DR AlphaFoldDB; O76879; -.
DR SMR; O76879; -.
DR STRING; 7227.FBpp0070480; -.
DR PaxDb; O76879; -.
DR PRIDE; O76879; -.
DR DNASU; 31252; -.
DR EnsemblMetazoa; FBtr0070504; FBpp0070480; FBgn0000092.
DR GeneID; 31252; -.
DR KEGG; dme:Dmel_CG2650; -.
DR CTD; 31252; -.
DR FlyBase; FBgn0000092; dyw.
DR VEuPathDB; VectorBase:FBgn0000092; -.
DR eggNOG; ENOG502T6I4; Eukaryota.
DR GeneTree; ENSGT00530000064246; -.
DR HOGENOM; CLU_069908_0_2_1; -.
DR InParanoid; O76879; -.
DR OMA; HTYLNIT; -.
DR OrthoDB; 1572898at2759; -.
DR PhylomeDB; O76879; -.
DR BioGRID-ORCS; 31252; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31252; -.
DR PRO; PR:O76879; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000092; Expressed in second segment of antenna (Drosophila) and 7 other tissues.
DR Genevisible; O76879; DM.
DR GO; GO:0005615; C:extracellular space; ISS:FlyBase.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0032504; P:multicellular organism reproduction; IBA:GO_Central.
DR GO; GO:0010841; P:positive regulation of circadian sleep/wake cycle, wakefulness; IMP:UniProtKB.
DR Gene3D; 3.15.10.30; -; 1.
DR InterPro; IPR010562; Haemolymph_juvenile_hormone-bd.
DR InterPro; IPR038606; To_sf.
DR Pfam; PF06585; JHBP; 1.
DR SMART; SM00700; JHBP; 1.
PE 2: Evidence at transcript level;
KW Biological rhythms; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..260
FT /note="Circadian clock-controlled protein daywake"
FT /id="PRO_0000020860"
FT VARIANT 219
FT /note="Q -> L (in strain: Oregon-R, wi4, wi15, wi6, wi24,
FT wi30, wi32 and wi33)"
FT /evidence="ECO:0000269|PubMed:11975710"
FT CONFLICT 64..70
FT /note="VAALEPI -> SGRAGAHS (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="G -> GSSPS (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 260 AA; 29179 MW; 8F2DA6F9A8E12323 CRC64;
MQLTGASMFL VWVGLLSWVS CRVDASEGFP SPLKRCKLQD ESCLLAQAQT FFQAFKNGIP
ERQVAALEPI ALGTMFIESG GHSESIKFKL TMSDAKLYNL ANSMMVKSLK GFTKDLTRPL
KLTLLLDNPE LEVRAKYDVD GKLLILPIVS KGDLTIRLND VHTKVWITAE PVKRSDGHTY
LNITDYKTAT KIKGGHFDLS NLFNDNKELR DSTLKVLNQE WSTLALDVQP KINEACAKAF
SAIVQSLWAN IPYDEFFEKE
