CCD12_HUMAN
ID CCD12_HUMAN Reviewed; 166 AA.
AC Q8WUD4; Q8N8I4;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Coiled-coil domain-containing protein 12;
GN Name=CCDC12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-94, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- INTERACTION:
CC Q8WUD4; Q9H1Y0: ATG5; NbExp=4; IntAct=EBI-2557572, EBI-1047414;
CC Q8WUD4; Q9BZJ0: CRNKL1; NbExp=2; IntAct=EBI-2557572, EBI-1049701;
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DR EMBL; AK096749; BAC04857.1; -; mRNA.
DR EMBL; BC020830; AAH20830.1; -; mRNA.
DR CCDS; CCDS2748.3; -.
DR RefSeq; NP_001264003.1; NM_001277074.1.
DR AlphaFoldDB; Q8WUD4; -.
DR SMR; Q8WUD4; -.
DR BioGRID; 127412; 106.
DR IntAct; Q8WUD4; 30.
DR MINT; Q8WUD4; -.
DR STRING; 9606.ENSP00000292314; -.
DR GlyGen; Q8WUD4; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8WUD4; -.
DR PhosphoSitePlus; Q8WUD4; -.
DR BioMuta; CCDC12; -.
DR DMDM; 74730697; -.
DR EPD; Q8WUD4; -.
DR jPOST; Q8WUD4; -.
DR MassIVE; Q8WUD4; -.
DR MaxQB; Q8WUD4; -.
DR PaxDb; Q8WUD4; -.
DR PeptideAtlas; Q8WUD4; -.
DR PRIDE; Q8WUD4; -.
DR ProteomicsDB; 74658; -.
DR Antibodypedia; 58026; 109 antibodies from 18 providers.
DR DNASU; 151903; -.
DR Ensembl; ENST00000425441.5; ENSP00000416263.2; ENSG00000160799.12.
DR Ensembl; ENST00000683445.1; ENSP00000508011.1; ENSG00000160799.12.
DR GeneID; 151903; -.
DR KEGG; hsa:151903; -.
DR MANE-Select; ENST00000683445.1; ENSP00000508011.1; NM_001277074.2; NP_001264003.1.
DR UCSC; uc062jbe.1; human.
DR CTD; 151903; -.
DR DisGeNET; 151903; -.
DR GeneCards; CCDC12; -.
DR HGNC; HGNC:28332; CCDC12.
DR HPA; ENSG00000160799; Low tissue specificity.
DR neXtProt; NX_Q8WUD4; -.
DR OpenTargets; ENSG00000160799; -.
DR PharmGKB; PA134970849; -.
DR VEuPathDB; HostDB:ENSG00000160799; -.
DR eggNOG; KOG3407; Eukaryota.
DR GeneTree; ENSGT00390000011619; -.
DR HOGENOM; CLU_091076_3_0_1; -.
DR InParanoid; Q8WUD4; -.
DR OrthoDB; 1604272at2759; -.
DR PhylomeDB; Q8WUD4; -.
DR PathwayCommons; Q8WUD4; -.
DR SignaLink; Q8WUD4; -.
DR BioGRID-ORCS; 151903; 234 hits in 1082 CRISPR screens.
DR ChiTaRS; CCDC12; human.
DR GenomeRNAi; 151903; -.
DR Pharos; Q8WUD4; Tdark.
DR PRO; PR:Q8WUD4; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8WUD4; protein.
DR Bgee; ENSG00000160799; Expressed in sural nerve and 186 other tissues.
DR ExpressionAtlas; Q8WUD4; baseline and differential.
DR Genevisible; Q8WUD4; HS.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR InterPro; IPR013169; mRNA_splic_Cwf18-like.
DR PANTHER; PTHR31551; PTHR31551; 1.
DR Pfam; PF08315; cwf18; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..166
FT /note="Coiled-coil domain-containing protein 12"
FT /id="PRO_0000076191"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 8..28
FT /evidence="ECO:0000255"
FT COILED 117..144
FT /evidence="ECO:0000255"
FT COMPBIAS 11..56
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT MOD_RES 53
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8R344"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT CROSSLNK 94
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CONFLICT 97
FT /note="P -> S (in Ref. 1; BAC04857)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 166 AA; 19181 MW; 8222968EF263F0AD CRC64;
MEATTAGVGR LEEEALRRKE RLKALREKTG RKDKEDGEPK TKHLREEEEE GEKHRELRLR
NYVPEDEDLK KRRVPQAKPV AVEEKVKEQL EAAKPEPVIE EVDLANLAPR KPDWDLKRDV
AKKLEKLKKR TQRAIAELIR ERLKGQEDSL ASAVDAATEQ KTCDSD
