CCD1D_DROME
ID CCD1D_DROME Reviewed; 521 AA.
AC Q7KR10; A9UNB6; Q95RF5;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Probable cytochrome P450 12d1 distal, mitochondrial;
DE EC=1.14.-.-;
DE AltName: Full=CYPXIID1;
DE Flags: Precursor;
GN Name=Cyp12d1-d {ECO:0000312|EMBL:AAG22287.3};
GN Synonyms=Cyp12d1 {ECO:0000250|UniProtKB:P82712}; ORFNames=CG33503;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAG22287.3}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAG22287.3}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAL28963.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-521.
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569}, and
RC Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=15663776; DOI=10.1111/j.1365-2583.2005.00532.x;
RA Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M.,
RA Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E.,
RA Pittendrigh B.R.;
RT "Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains
RT of Drosophila melanogaster.";
RL Insect Mol. Biol. 14:69-77(2005).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Only expressed in adults. Expression varies in DDT
CC resistant strains (Wisconsin, 91-R and Hikone-R).
CC {ECO:0000269|PubMed:15663776}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28963.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAG22287.3; -; Genomic_DNA.
DR EMBL; BT031280; ABY20521.1; -; mRNA.
DR EMBL; AY061415; AAL28963.1; ALT_INIT; mRNA.
DR EMBL; BT001433; AAN71188.1; -; mRNA.
DR RefSeq; NP_995812.1; NM_206090.2.
DR AlphaFoldDB; Q7KR10; -.
DR SMR; Q7KR10; -.
DR IntAct; Q7KR10; 1.
DR STRING; 7227.FBpp0088436; -.
DR PaxDb; Q7KR10; -.
DR PRIDE; Q7KR10; -.
DR DNASU; 2768720; -.
DR EnsemblMetazoa; FBtr0089418; FBpp0088436; FBgn0053503.
DR GeneID; 2768720; -.
DR KEGG; dme:Dmel_CG33503; -.
DR UCSC; CG33503-RA; d. melanogaster.
DR CTD; 2768720; -.
DR FlyBase; FBgn0053503; Cyp12d1-d.
DR VEuPathDB; VectorBase:FBgn0053503; -.
DR eggNOG; KOG0159; Eukaryota.
DR GeneTree; ENSGT00940000165868; -.
DR HOGENOM; CLU_001570_28_0_1; -.
DR InParanoid; Q7KR10; -.
DR OMA; WRANRRT; -.
DR OrthoDB; 825914at2759; -.
DR PhylomeDB; Q7KR10; -.
DR BioGRID-ORCS; 2768720; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Cyp12d1-p; fly.
DR GenomeRNAi; 2768720; -.
DR PRO; PR:Q7KR10; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0053503; Expressed in nurse follicle cell (Drosophila) and 18 other tissues.
DR Genevisible; Q7KR10; DM.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0030342; F:1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity; ISS:UniProtKB.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0046680; P:response to DDT; IMP:FlyBase.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR GO; GO:0042369; P:vitamin D catabolic process; ISS:UniProtKB.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Mitochondrion; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transit peptide.
FT TRANSIT 1..19
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 20..521
FT /note="Probable cytochrome P450 12d1 distal, mitochondrial"
FT /id="PRO_0000042639"
FT BINDING 467
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P82712"
FT CONFLICT 94
FT /note="W -> S (in Ref. 4; AAN71188)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 521 AA; 60275 MW; 8771743CDE8AC4EB CRC64;
MNTLSSARSV AIYVGPVRSS RSASVLAHEQ AKSSITEEHK TYDEIPRPNK FKFMRAFMPG
GEFQNASITE YTSAMRKRYG DIYVMPGMFG RKDWVTTFNT KDIEMVFRNE GIWPRRDGLD
SIVYFREHVR PDVYGEVQGL VASQNEAWGK LRSAINPIFM QPRGLRMYYE PLSNINNEFI
ERIKEIRDPK TLEVPEDFTD EISRLVFESL GLVAFDRQMG LIRKNRDNSD ALTLFQTSRD
IFRLTFKLDI QPSMWKIIST PTYRKMKRTL NDSLNVSQKM LKENQDALEK RRQAGEKINS
NSMLERLMEI DPKVAVIMSL DILFAGVDAT ATLLSAVLLC LSKHPDKQAK LREELLSIMP
TKDSLLNEEN MKDMPYLRAV IKETLRYYPN GFGTMRTCQN DVILSGYRVP KGTTVLLGSN
VLMKEATYYP RPDEFLPERW LRDPETGKKM QVSPFTFLPF GFGPRMCIGK RVVDLEMETT
VAKLIRNFHV EFNRDASRPF KTMFLMEPAI TFPFKFTDIE Q
