CCD1P_DROME
ID CCD1P_DROME Reviewed; 521 AA.
AC P82712; Q8IH46; Q8T4K3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Probable cytochrome P450 12d1 proximal, mitochondrial;
DE EC=1.14.-.-;
DE AltName: Full=CYPXIID1;
DE Flags: Precursor;
GN Name=Cyp12d1-p; Synonyms=Cyp12d1; ORFNames=CG30489;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Buzeli R.A., Pedra J.H.F., Scharf M., Pittendrigh B.R.;
RT "Drosophila melanogaster CYP12D1 complete cDNA.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=15663776; DOI=10.1111/j.1365-2583.2005.00532.x;
RA Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M.,
RA Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E.,
RA Pittendrigh B.R.;
RT "Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains
RT of Drosophila melanogaster.";
RL Insect Mol. Biol. 14:69-77(2005).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Only expressed in adults. Expression varies in DDT
CC resistant strains (Wisconsin, 91-R and Hikone-R).
CC {ECO:0000269|PubMed:15663776}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY081961; AAL89789.1; -; mRNA.
DR EMBL; AE013599; AAM68745.1; -; Genomic_DNA.
DR RefSeq; NP_001286304.1; NM_001299375.1.
DR RefSeq; NP_610635.4; NM_136791.6.
DR AlphaFoldDB; P82712; -.
DR SMR; P82712; -.
DR BioGRID; 73191; 64.
DR IntAct; P82712; 1.
DR STRING; 7227.FBpp0088437; -.
DR PaxDb; P82712; -.
DR PRIDE; P82712; -.
DR EnsemblMetazoa; FBtr0089419; FBpp0088437; FBgn0050489.
DR EnsemblMetazoa; FBtr0345674; FBpp0311725; FBgn0050489.
DR GeneID; 246648; -.
DR KEGG; dme:Dmel_CG30489; -.
DR UCSC; CG30489-RA; d. melanogaster.
DR CTD; 246648; -.
DR FlyBase; FBgn0050489; Cyp12d1-p.
DR VEuPathDB; VectorBase:FBgn0050489; -.
DR eggNOG; KOG0159; Eukaryota.
DR GeneTree; ENSGT00940000165868; -.
DR HOGENOM; CLU_001570_28_0_1; -.
DR InParanoid; P82712; -.
DR OMA; VNPVFMQ; -.
DR OrthoDB; 825914at2759; -.
DR PhylomeDB; P82712; -.
DR BioGRID-ORCS; 246648; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Cyp12d1-p; fly.
DR GenomeRNAi; 246648; -.
DR PRO; PR:P82712; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0050489; Expressed in Malpighian tubule and 24 other tissues.
DR ExpressionAtlas; P82712; baseline and differential.
DR Genevisible; P82712; DM.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0046680; P:response to DDT; IMP:FlyBase.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Mitochondrion; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transit peptide.
FT TRANSIT 1..19
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 20..521
FT /note="Probable cytochrome P450 12d1 proximal,
FT mitochondrial"
FT /id="PRO_0000003613"
FT BINDING 467
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 277
FT /note="A -> S (in Ref. 1; AAL89789)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="L -> F (in Ref. 1; AAL89789)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 521 AA; 60211 MW; 8FCD563239153B1A CRC64;
MNTLSSARSV AIYVGPVRSS RSASVLAHEQ AKSSITEEHK TYDEIPRPNK FKFMRAFMPG
GEFQNASITE YTSAMRKRYG DIYVMPGMFG RKDWVTTFNT KDIEMVFRNE GIWPRRDGLD
SIVYFREHVR PDVYGEVQGL VASQNEAWGK LRSAINPIFM QPRGLRMYYE PLSNINNEFI
ERIKEIRDPK TLEVPEDFTD EISRLVFESL GLVAFDRQMG LIRKNRDNSD ALTLFQTSRD
IFRLTFKLDI QPSMWKIIST PTYRKMKRTL NDSLNVAQKM LKENQDALEK RRQAGEKINS
NSMLERLMEI DPKVAVIMSL DILFAGVDAT ATLLSAVLLC LSKHPDKQAK LREELLSIMP
TKDSLLNEEN MKDMPYLRAV IKETLRYYPN GLGTMRTCQN DVILSGYRVP KGTTVLLGSN
VLMKEATYYP RPDEFLPERW LRDPETGKKM QVSPFTFLPF GFGPRMCIGK RVVDLEMETT
VAKLIRNFHV EFNRDASRPF KTMFVMEPAI TFPFKFTDIE Q
