ID   CCD1_ONCHC              Reviewed;         562 AA.
AC   C3VEQ4;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Carotenoid 9,10(9',10')-cleavage dioxygenase 1;
DE            EC=1.14.99.n4;
DE   AltName: Full=Carotenoid cleavage dioxygenase 1;
DE            Short=OgCCD1;
GN   Name=CCD1;
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Cleaves a variety of carotenoids symmetrically at both the 9-
CC       10 and 9'-10' double bonds. Catalyzes the formation of 4,9-
CC       dimethyldodeca-2,4,6,8,10-pentaene-1,12-dialdehyde and probably
CC       hydroxydihydro-beta-ionone from zeaxanthin (By similarity).
CC       {ECO:0000250, ECO:0000269|PubMed:20635095}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 O2 = 2 (3R)-hydroxy-beta-ionone +
CC         4,9-dimethyldodeca-2,4,6,8,10-pentaenedial; Xref=Rhea:RHEA:26393,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:27547, ChEBI:CHEBI:53171,
CC         ChEBI:CHEBI:53173; EC=1.14.99.n4;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Probably on the
CC       exterior surface of the plastids.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves. Detected in roots and flower
CC       buds. {ECO:0000269|PubMed:20635095}.
CC   -!- MISCELLANEOUS: The carotenoid cleavage dioxygenase 1 (CCD1), which
CC       catabolizes carotenoid metabolites, is up-regulated in the floral
CC       tissues of the White Jade cultivar, resulting in a pure white flower.
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FJ859995; ACP27629.1; -; mRNA.
DR   AlphaFoldDB; C3VEQ4; -.
DR   SMR; C3VEQ4; -.
DR   PRIDE; C3VEQ4; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; PTHR10543; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Dioxygenase; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN           1..562
FT                   /note="Carotenoid 9,10(9',10')-cleavage dioxygenase 1"
FT                   /id="PRO_0000426715"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        13..28
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         243
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         291
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         356
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         546
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  63412 MW;  CA7DD67400693311 CRC64;
     MGESASTETL EKKQPFTAEE EAADGKKSHR SVVAVQPKPR KGILSSTIDL IEKVIVYFAH
     DSSKPLHYLT GNFAPVRDET PPFAALTVHG SLPVCLNGEF VRVGPNPKFS PVAGYHWFDG
     DGMIHGLRIK DGKATYVSRY VKTSKLMQEE YFEGAKFMKI GDLKGLFGLF MVQMELLRAK
     LKILDVSYGR STANTAMIYH HGKLLALSEG DKPYVIKVLE DGDLQTLGLL DYNKRLAHSF
     TAHPKVDPFT DEMFTFGYSH TSPYITYRVI TKDGVMLNPV PITIPEPIMM HDFAITENYA
     IFLDLPLYFR PKETIKGKLI FTFDPTKKAK FGVLPRYAKD EQLIRWFDLP NCFIFHNANA
     WEDGDEVVLI TCRLKNIDLD LVNGAVKEKL ENFSNQLYEM RFNMKTGAAT QKELSAPMVD
     FPRINENYTG RKQRYVYCTS FANIDKVNGI VKFDLHAEPE TGKKVLEVGG NVLGIFDLGP
     GRFGSEAVFV PREAGTRVEE DDGYLIFFVY DETTGESKVY VIDAKTMSPE PVAVVDLPCR
     VPYGFHAFFV NEEQIQKQQA EI