ID   CCD1_PEA                Reviewed;         541 AA.
AC   Q8LP17;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=Carotenoid 9,10(9',10')-cleavage dioxygenase 1;
DE            EC=1.14.99.n4;
DE   AltName: Full=PsCCD1;
DE   AltName: Full=PsNCED1;
GN   Name=CCD1; Synonyms=NCED1;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Alaska; TISSUE=Axillary bud;
RA   Nakako A., Mori H.;
RT   "Isolation and characterization of a cDNA encoding a nine-cis-
RT   epoxycarotenoid dioxygenase 1 (PsNCED1) from Pisum sativum.";
RL   Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves a variety of carotenoids at the 9-10 and 9'-10'
CC       double bonds. Probably not involved in abscisic acid biosynthesis (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 O2 = 2 (3R)-hydroxy-beta-ionone +
CC         4,9-dimethyldodeca-2,4,6,8,10-pentaenedial; Xref=Rhea:RHEA:26393,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:27547, ChEBI:CHEBI:53171,
CC         ChEBI:CHEBI:53173; EC=1.14.99.n4;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR   EMBL; AB080191; BAC10549.1; -; mRNA.
DR   AlphaFoldDB; Q8LP17; -.
DR   SMR; Q8LP17; -.
DR   PRIDE; Q8LP17; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; PTHR10543; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN           1..541
FT                   /note="Carotenoid 9,10(9',10')-cleavage dioxygenase 1"
FT                   /id="PRO_0000285989"
FT   BINDING         222
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         270
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         336
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         526
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   541 AA;  61226 MW;  71C7675C841A0E79 CRC64;
     MGSEKKENGV ILEVEPKPSN GFTSKAVDLL EKIIVKLFYD SSLPHHWLSG NFAPVKDETP
     PVKDLTVQGH LPDCLNGEFV RVGPNPKFSP VAGYHWFDGD GMIHGLRIKD GKASYVSRFV
     KTSRFKQEEY FNGSKFMKIG DLKGLFGLLM VNMQMLRAKL KILDVSYGHG TANTALVYHH
     QKLLALSEGD KPYAIKVFED GDLQTLGMLD YDKRLGHNFT AHPKVDPFTG EMFTFGYSHT
     APYVTYRVIS KDGFMQDPVP ITISDPVMMH DFAITENYSI FMDLPLYFRP KEMVKNKTLI
     FSFDSTKKAR FGVLPRYAKD DKHIRWFELP NCFIFHNANA WEEEDEIVLI TCRLENPNLD
     VVGGAVKEKL DNFSNELYEM RFNMKTGEAS QKKLSASTVD FPRVNESYTG RKQRYVYGTT
     LDSIAKVTGI IKFDLHAEPD SGKTKLEVGG NVQGLYDLGP GRFGSEAVYV PRVPGTDSEE
     DDGYLIFFVH DENTGKSFVH VIDAKRMSAE PVAVVELPQR VPYGFHAFFV TEDQLQEQAK
     F