CCD39_CHLRE
ID CCD39_CHLRE Reviewed; 894 AA.
AC A8IQE0;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 44.
DE RecName: Full=Coiled-coil domain-containing protein 39 {ECO:0000305};
DE AltName: Full=Flagellar-associated protein 59 {ECO:0000303|PubMed:15998802};
GN Name=CCDC39; Synonyms=FAP59 {ECO:0000303|PubMed:15998802};
GN ORFNames=CHLREDRAFT_189109 {ECO:0000312|EMBL:EDP04876.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503, and cw92;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15998802; DOI=10.1083/jcb.200504008;
RA Pazour G.J., Agrin N., Leszyk J., Witman G.B.;
RT "Proteomic analysis of a eukaryotic cilium.";
RL J. Cell Biol. 170:103-113(2005).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CCDC40/FAP172, DISRUPTION
RP PHENOTYPE, AND PHOSPHORYLATION.
RX PubMed=25395538; DOI=10.1126/science.1260214;
RA Oda T., Yanagisawa H., Kamiya R., Kikkawa M.;
RT "Cilia and flagella. A molecular ruler determines the repeat length in
RT eukaryotic cilia and flagella.";
RL Science 346:857-860(2014).
CC -!- FUNCTION: Required for assembly of dynein regulatory complex (DRC) and
CC inner dynein arm complexes, which are responsible for ciliary beat
CC regulation, by acting as a molecular ruler that determines the 96
CC nanometer (nm) repeat length and arrangements of components in cilia
CC and flagella (PubMed:25395538). Together with CCDC40/FAP172 forms a 96-
CC nm-long complex in flagella. This complex does not act as a physical
CC ruler, but rather act as a negative regulator for radial spokes: the
CC complex lays along specific protofilaments, masking radial spoke
CC binding sites and allowing recruitment of inner dynein arm (IDA) and
CC nexin-dynein regulatory complexes (N-DRC) (PubMed:25395538).
CC {ECO:0000269|PubMed:25395538}.
CC -!- SUBUNIT: Interacts with CCDC40/FAP172. {ECO:0000269|PubMed:25395538}.
CC -!- INTERACTION:
CC A8IQE0; A8IQT2: CCDC40; NbExp=2; IntAct=EBI-16127597, EBI-16127612;
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:25395538}.
CC -!- PTM: Phosphorylated in flagella. {ECO:0000269|PubMed:25395538}.
CC -!- DISRUPTION PHENOTYPE: Short and immotile flagella. Inner dynein arm
CC (IDA) and nexin-dynein regulatory complex (N-DRC) components are absent
CC or severely reduced. Radial spokes are attached to doublet microtubules
CC with an irregular periodicity of 32 nm instead of 96 nm.
CC {ECO:0000269|PubMed:25395538}.
CC -!- SIMILARITY: Belongs to the CCDC39 family. {ECO:0000305}.
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DR EMBL; DS496120; EDP04876.1; -; Genomic_DNA.
DR RefSeq; XP_001691768.1; XM_001691716.1.
DR AlphaFoldDB; A8IQE0; -.
DR SMR; A8IQE0; -.
DR DIP; DIP-61434N; -.
DR IntAct; A8IQE0; 1.
DR STRING; 3055.EDP04876; -.
DR PaxDb; A8IQE0; -.
DR PRIDE; A8IQE0; -.
DR GeneID; 5717298; -.
DR eggNOG; ENOG502QS0D; Eukaryota.
DR HOGENOM; CLU_009793_2_0_1; -.
DR InParanoid; A8IQE0; -.
DR OrthoDB; 355514at2759; -.
DR GO; GO:0005930; C:axoneme; IEA:InterPro.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0003341; P:cilium movement; IEA:InterPro.
DR GO; GO:0036159; P:inner dynein arm assembly; IEA:InterPro.
DR InterPro; IPR033290; CCDC39.
DR PANTHER; PTHR18962; PTHR18962; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Flagellum; Phosphoprotein.
FT CHAIN 1..894
FT /note="Coiled-coil domain-containing protein 39"
FT /id="PRO_0000405820"
FT REGION 844..894
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 32..143
FT /evidence="ECO:0000255"
FT COILED 187..411
FT /evidence="ECO:0000255"
FT COILED 461..609
FT /evidence="ECO:0000255"
FT COILED 647..788
FT /evidence="ECO:0000255"
FT COMPBIAS 846..879
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 894 AA; 101488 MW; 2E0A53532FA9E717 CRC64;
MANIDPYRTE EELVEDDEEV DMSFLPPFAK GTENEVLYVE NARMERRLER TERALETNMD
RLHIMDEHLK NVQQELKYTQ TRVEAKNKEI ESEKHLNAMA EREMGRLKKD IGKMEAERQE
LADKINGLQN QIYKNNEKLD QFKMLMNWNQ EELEQWALAE RQKAEDNAAL EKYRHADDGK
VKELTLALER VSKQVVGRKE ELEAEVVETQ AAQIQLDKAA EDFRKLHVER QDLIRQWEEA
VEAMRHRDAA IAAASEQFAM QKDVLRERKR ELDAQARFLE NETLNTKEAD ARVAYYEREV
GKQRDVLARE QARTEELNNQ VELVKATLSK AATELAQRTV ENKQAREDLD AKRQKLDAAR
KRFVVLKRKL ENEFGNLDSM EAKASELEAM RRGEEARLKA ILKEHELLKK EQYKRSQVLF
DLRQKERELI SEISGGQGQN KNLAARIHAL DEQVVARAGG VRSEEETRAL NARIEKLTAI
LEGVKRAEDD LLAARRANTS LRADRAKLDE TISTLKLEND MVSRQVKGSV EAREKALVDH
DVLALEVKRL RDILAAHADE VFSLENRKQQ LALSMEERKQ EVEVHRDGLR AELRLLREDV
HRITLELKER LLRCEKLQAK FEIISAKHRG SGEDDGEERT QAYYVIKAAQ EREALQREGD
DLDGRIRVAE KEVAALEATL AQLMAVNTNF AASYKKVGSK EAFEERAALR DKLDKAYDKL
KARRADEAAI AGDIQVSEAR LSNLGQEQRS LQALVDDMTR RKAEAQRQLD EQREKLGRAL
GRTDKLRQKL GLANSPQGAD VELAEVRDVT RAMLLELKAL ALANPGAMIA EACEAAGIRL
PSGGSNPPSL GGSRPGSARS QTSLGSVRSA RSVASQQRGG MGGSPAVRTI QLGA
