CCD43_HUMAN
ID CCD43_HUMAN Reviewed; 224 AA.
AC Q96MW1; C9JVK9;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Coiled-coil domain-containing protein 43;
GN Name=CCDC43;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Small intestine;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-95, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25114211; DOI=10.1073/pnas.1413825111;
RA Impens F., Radoshevich L., Cossart P., Ribet D.;
RT "Mapping of SUMO sites and analysis of SUMOylation changes induced by
RT external stimuli.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:12432-12437(2014).
CC -!- INTERACTION:
CC Q96MW1; O95198: KLHL2; NbExp=3; IntAct=EBI-9247198, EBI-746999;
CC Q96MW1; Q9BSI4: TINF2; NbExp=2; IntAct=EBI-9247198, EBI-717399;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96MW1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96MW1-2; Sequence=VSP_040750;
CC -!- SIMILARITY: Belongs to the CCDC43 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK056357; BAB71162.1; -; mRNA.
DR EMBL; BX648868; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC091152; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS45704.1; -. [Q96MW1-1]
DR CCDS; CCDS45705.1; -. [Q96MW1-2]
DR RefSeq; NP_001092695.1; NM_001099225.1. [Q96MW1-2]
DR RefSeq; NP_653210.2; NM_144609.2. [Q96MW1-1]
DR AlphaFoldDB; Q96MW1; -.
DR BioGRID; 125890; 44.
DR IntAct; Q96MW1; 8.
DR MINT; Q96MW1; -.
DR STRING; 9606.ENSP00000323782; -.
DR iPTMnet; Q96MW1; -.
DR PhosphoSitePlus; Q96MW1; -.
DR BioMuta; CCDC43; -.
DR DMDM; 296434426; -.
DR EPD; Q96MW1; -.
DR jPOST; Q96MW1; -.
DR MassIVE; Q96MW1; -.
DR MaxQB; Q96MW1; -.
DR PaxDb; Q96MW1; -.
DR PeptideAtlas; Q96MW1; -.
DR PRIDE; Q96MW1; -.
DR ProteomicsDB; 77422; -. [Q96MW1-1]
DR ProteomicsDB; 77423; -. [Q96MW1-2]
DR Antibodypedia; 8262; 76 antibodies from 15 providers.
DR DNASU; 124808; -.
DR Ensembl; ENST00000315286.13; ENSP00000323782.7; ENSG00000180329.14. [Q96MW1-1]
DR Ensembl; ENST00000457422.6; ENSP00000400845.1; ENSG00000180329.14. [Q96MW1-2]
DR GeneID; 124808; -.
DR KEGG; hsa:124808; -.
DR MANE-Select; ENST00000315286.13; ENSP00000323782.7; NM_144609.3; NP_653210.2.
DR UCSC; uc002ihc.3; human. [Q96MW1-1]
DR CTD; 124808; -.
DR DisGeNET; 124808; -.
DR GeneCards; CCDC43; -.
DR HGNC; HGNC:26472; CCDC43.
DR HPA; ENSG00000180329; Tissue enhanced (skeletal).
DR neXtProt; NX_Q96MW1; -.
DR OpenTargets; ENSG00000180329; -.
DR PharmGKB; PA142672160; -.
DR VEuPathDB; HostDB:ENSG00000180329; -.
DR eggNOG; ENOG502RYDM; Eukaryota.
DR GeneTree; ENSGT00390000015009; -.
DR HOGENOM; CLU_079381_2_0_1; -.
DR InParanoid; Q96MW1; -.
DR OrthoDB; 1503148at2759; -.
DR PhylomeDB; Q96MW1; -.
DR TreeFam; TF324859; -.
DR PathwayCommons; Q96MW1; -.
DR SignaLink; Q96MW1; -.
DR BioGRID-ORCS; 124808; 53 hits in 1081 CRISPR screens.
DR ChiTaRS; CCDC43; human.
DR GenomeRNAi; 124808; -.
DR Pharos; Q96MW1; Tdark.
DR PRO; PR:Q96MW1; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q96MW1; protein.
DR Bgee; ENSG00000180329; Expressed in deltoid and 179 other tissues.
DR ExpressionAtlas; Q96MW1; baseline and differential.
DR Genevisible; Q96MW1; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR InterPro; IPR037666; CCDC43.
DR PANTHER; PTHR31684; PTHR31684; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..224
FT /note="Coiled-coil domain-containing protein 43"
FT /id="PRO_0000234500"
FT REGION 138..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 176..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 121..145
FT /evidence="ECO:0000255"
FT COILED 177..218
FT /evidence="ECO:0000255"
FT COMPBIAS 176..215
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 139
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT CROSSLNK 95
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1)"
FT /evidence="ECO:0007744|PubMed:25114211"
FT VAR_SEQ 144..224
FT /note="EADEKDDSGATTMNIGSDKLLFRNTNVEDVLNARKLERDSLRDESQRKKEQD
FT KLQRERDKLAKQERKEKEKKRTQRGERKR -> SVPKHQCGRCP (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_040750"
FT CONFLICT 174
FT /note="L -> F (in Ref. 1; BAB71162)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 224 AA; 25248 MW; ED4E1CB196FEEFA7 CRC64;
MAAPSEVAAI APGEGDGGGG GFGSWLDGRL EALGVDRAVY GAYILGILQE EEEEEKLDAL
QGILSAFLEE DSLLNICKEI VERWSETQNV VTKVKKEDEV QAIATLIEKQ AQIVVKPRMV
SEEEKQRKAA LLAQYADVTD EEDEADEKDD SGATTMNIGS DKLLFRNTNV EDVLNARKLE
RDSLRDESQR KKEQDKLQRE RDKLAKQERK EKEKKRTQRG ERKR
