CCD61_CHLRE
ID CCD61_CHLRE Reviewed; 603 AA.
AC Q68UI8;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Variable flagella 3 {ECO:0000303|PubMed:32375023};
DE AltName: Full=Coiled-coil domain-containing protein 61 {ECO:0000303|PubMed:32375023};
GN Name=VFL3 {ECO:0000303|Ref.1};
GN Synonyms=CCDC61 {ECO:0000303|PubMed:32375023};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=21gr / CC-1690;
RA Iyadurai K.B., Sislo R.A., Silflow C.D.;
RT "Role of the VFL3 Gene in Segregation and Rotational Positioning of Basal
RT Bodies in Chlamydomonas.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF 126-PHE-ASP-127;
RP LYS-266; ARG-270; LYS-273; ARG-275 AND ARG-277.
RX PubMed=32375023; DOI=10.1016/j.str.2020.04.010;
RA Ochi T., Quarantotti V., Lin H., Jullien J., Rosa e Silva I., Boselli F.,
RA Barnabas D.D., Johnson C.M., McLaughlin S.H., Freund S.M.V.,
RA Blackford A.N., Kimata Y., Goldstein R.E., Jackson S.P., Blundell T.L.,
RA Dutcher S.K., Gergely F., van Breugel M.;
RT "CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.";
RL Structure 28:674-689(2020).
CC -!- FUNCTION: Required for normal flagella and striated fiber formation.
CC {ECO:0000269|PubMed:32375023}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum basal body
CC {ECO:0000269|PubMed:32375023}. Note=Localizes to basal bodies and the
CC proximal ends of flagella. {ECO:0000269|PubMed:32375023}.
CC -!- DOMAIN: The coiled-coil domain is involved in microtubule-binding.
CC {ECO:0000269|PubMed:32375023}.
CC -!- SIMILARITY: Belongs to the CCDC61 family. {ECO:0000305}.
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DR EMBL; AY328089; AAQ95705.1; -; Genomic_DNA.
DR EMBL; AY328090; AAQ95706.1; -; mRNA.
DR EMBL; DS496133; EDP01566.1; -; Genomic_DNA.
DR EMBL; CM008967; PNW82459.1; -; Genomic_DNA.
DR RefSeq; XP_001695308.1; XM_001695256.1.
DR AlphaFoldDB; Q68UI8; -.
DR SMR; Q68UI8; -.
DR STRING; 3055.EDP01566; -.
DR PaxDb; Q68UI8; -.
DR PRIDE; Q68UI8; -.
DR EnsemblPlants; PNW82459; PNW82459; CHLRE_06g279900v5.
DR GeneID; 5720899; -.
DR Gramene; PNW82459; PNW82459; CHLRE_06g279900v5.
DR KEGG; cre:CHLRE_06g279900v5; -.
DR eggNOG; ENOG502QRAS; Eukaryota.
DR HOGENOM; CLU_452976_0_0_1; -.
DR InParanoid; Q68UI8; -.
DR OMA; THRIKDK; -.
DR OrthoDB; 1106887at2759; -.
DR Proteomes; UP000006906; Chromosome 6.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IMP:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; IMP:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome.
FT CHAIN 1..603
FT /note="Variable flagella 3"
FT /id="PRO_0000451584"
FT REGION 288..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 539..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 169..289
FT /evidence="ECO:0000255"
FT COMPBIAS 297..354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..383
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 419..434
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 126..127
FT /note="FD->EA: Partially restores flagella formation in
FT VFL3 mutant strains and does not affect localization at the
FT cilium basal body."
FT /evidence="ECO:0000269|PubMed:32375023"
FT MUTAGEN 266
FT /note="K->E: Does not restore flagella formation in VFL3
FT mutant strains and does not affect localization at the
FT cilium basal body; when associated with E-270, E-273, E-275
FT and E-277."
FT /evidence="ECO:0000269|PubMed:32375023"
FT MUTAGEN 270
FT /note="R->E: Does not restore flagella formation in VFL3
FT mutant strains and does not affect localization at the
FT cilium basal body; when associated with E-266, E-273, E-275
FT and E-277."
FT /evidence="ECO:0000269|PubMed:32375023"
FT MUTAGEN 273
FT /note="K->E: Does not restore flagella formation in VFL3
FT mutant strain and does not affect localization at the
FT cilium basal body; when associated with E-266, E-270, E-275
FT and E-277."
FT /evidence="ECO:0000269|PubMed:32375023"
FT MUTAGEN 275
FT /note="R->E: Does not restore flagella formation in VFL3
FT mutant strains and does not affect localization at the
FT cilium basal body; when associated with E-266, E-270, E-273
FT and E-277."
FT /evidence="ECO:0000269|PubMed:32375023"
FT MUTAGEN 277
FT /note="R->E: Does not restore flagella formation in VFL3
FT mutant strains and does not affect localization at the
FT cilium basal body; when associated with E-266, E-270, E-273
FT and E-275."
FT /evidence="ECO:0000269|PubMed:32375023"
SQ SEQUENCE 603 AA; 65214 MW; FB8007C0154EF9A2 CRC64;
MGDICETVEA TFHGVAYLLT VSTIEGETLC VEVEQKSDCS RWRGDFTSRY IEDITAKTGN
FKKFPVFVKM LLSALKQASD SVFVDLLTYQ DLEVLKSRKA GGQAPPPRTQ PPNNKRYLIM
TYAAEFDRVH YPLPLLFEEN PDPQHLKRII SQLRSEVEGL QVEAGNRRGG EVSAELRRLR
EENAALQRQL KQMERSGSGV GAGAESSEAR ELARELRTVR KERDLLQARV EAAEAELERE
RGLHRRELRR RAKEQQELVD ELGRCKEQIR ELKMRIRQLT EDLEARDRRM NSTDRIRSVY
ARGSNDGSTS GVAPRRPSSG GRGPSSNYMA PTRGADSRSN SRGRGTSSAE RSRPNSAPIG
GPRPRFDPTE YVRQRKERES AAVGGRRSNA PTPPRSAGTS RASSVVGSRP ASAERLPPIR
TASPTNSRPS STERYRPGSG GPPGRTSAGG SRGAADRAGA RGPDPYAPRS RGASPSGRAT
AWGEPGASGG GAGGWSKFPG GGGGGVGGSG QRISSNSPRS MSPARALAEV KQKLSTFVAG
RGGSLQGDDA SSAHGEHMSQ SSKSQVFEDA TAEIADIDSR LHALQNFLRM AKTSSTSTST
TQA
