CCD63_MOUSE
ID CCD63_MOUSE Reviewed; 558 AA.
AC Q8CDV6; Q0VF78;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Coiled-coil domain-containing protein 63 {ECO:0000312|MGI:MGI:3607777};
GN Name=Ccdc63 {ECO:0000312|MGI:MGI:3607777};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=26501274; DOI=10.3390/ijms161024732;
RA Young S.A., Miyata H., Satouh Y., Kato H., Nozawa K., Isotani A.,
RA Aitken R.J., Baker M.A., Ikawa M.;
RT "CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified
RT Ccdc63 as essential for spermiogenesis.";
RL Int. J. Mol. Sci. 16:24732-24750(2015).
CC -!- FUNCTION: Plays a role in spermiogenesis (PubMed:26501274). Involved in
CC the elongation of flagella and the formation of sperm heads
CC (PubMed:26501274). {ECO:0000269|PubMed:26501274}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CDV6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CDV6-2; Sequence=VSP_025804;
CC -!- TISSUE SPECIFICITY: Expressed in testis, brain, thymus and lung
CC (PubMed:26501274). {ECO:0000269|PubMed:26501274}.
CC -!- DISRUPTION PHENOTYPE: Male mice are infertile despite regular
CC copulation. Display shortened sperm tails and malformed sperm heads and
CC motionless spermatozoa (PubMed:26501274). The 9+2 structure of the
CC axoneme seems to be intact (PubMed:26501274). Spermatozoa are able to
CC activate oocytes when used in intracytoplasmic sperm injection (ICSI)
CC and these embryos are able to develop to term (PubMed:26501274).
CC {ECO:0000269|PubMed:26501274}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC26487.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK029513; BAC26487.1; ALT_INIT; mRNA.
DR EMBL; BC118945; AAI18946.1; -; mRNA.
DR RefSeq; NP_001276738.1; NM_001289809.1.
DR RefSeq; NP_001276740.1; NM_001289811.1. [Q8CDV6-2]
DR RefSeq; NP_899130.1; NM_183307.4.
DR RefSeq; XP_006530451.1; XM_006530388.3.
DR AlphaFoldDB; Q8CDV6; -.
DR SMR; Q8CDV6; -.
DR BioGRID; 236918; 3.
DR STRING; 10090.ENSMUSP00000050582; -.
DR iPTMnet; Q8CDV6; -.
DR PhosphoSitePlus; Q8CDV6; -.
DR PaxDb; Q8CDV6; -.
DR PRIDE; Q8CDV6; -.
DR ProteomicsDB; 281313; -. [Q8CDV6-1]
DR ProteomicsDB; 281314; -. [Q8CDV6-2]
DR GeneID; 330188; -.
DR KEGG; mmu:330188; -.
DR UCSC; uc008zkq.2; mouse. [Q8CDV6-1]
DR CTD; 160762; -.
DR MGI; MGI:3607777; Ccdc63.
DR eggNOG; ENOG502QSIU; Eukaryota.
DR InParanoid; Q8CDV6; -.
DR OrthoDB; 648380at2759; -.
DR PhylomeDB; Q8CDV6; -.
DR TreeFam; TF323742; -.
DR BioGRID-ORCS; 330188; 1 hit in 70 CRISPR screens.
DR ChiTaRS; Ccdc63; mouse.
DR PRO; PR:Q8CDV6; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8CDV6; protein.
DR GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR GO; GO:0003341; P:cilium movement; IBA:GO_Central.
DR GO; GO:0036158; P:outer dynein arm assembly; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR InterPro; IPR029652; CCDC63_fam.
DR PANTHER; PTHR21694:SF18; PTHR21694:SF18; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Differentiation; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..558
FT /note="Coiled-coil domain-containing protein 63"
FT /id="PRO_0000288875"
FT COILED 10..44
FT /evidence="ECO:0000255"
FT COILED 185..259
FT /evidence="ECO:0000255"
FT COILED 369..414
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..71
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025804"
SQ SEQUENCE 558 AA; 65274 MW; 876693B75800C197 CRC64;
MKKVRRKGSS GLSELSEKAR EQLAQAELRK LRQQFRKMVD SRKSFNFRHQ RMIAGQYKEI
ETLKAEQAET TMLLSLVKSP KNLDINQKNF MELRFLLQTK GDYEALISSM KVLLGELDDK
IVQMERKITN QRQLFLRTQE ANNPKRLQRQ IHVLESRLNL VTVHFDTMLT SNSQLRKEIE
DLLFEKAAYD HVYQQLQRRL QTQKKTMNVA IEQSAQAYEQ RVEAMARMAA MKDRQQKDIS
QYNLEIRELE RLYDHETKLK SFLLAKLNDR SEFEDQAKKQ EDVKSKKLGK KGKGESFASY
EVAHLRLLKL AENGDLNQLT EDFLAKEEKN FARFTYVTEL NNDMETMHKK TQRIQDDIIN
LRSQQQTSHE GTRSILKQME EKLRKTTQEA DIYETKYKEM SKTLEYLKNS VEKMFKKINC
DATEILGKLG ESGKVTDINL QQYFAIIEKK TNDLLLLESF RRLQEAEGPD VDVPQPFVNP
FWGGSALLKP PEPIRVVLPV FGADSFSDKL EEVDSPLDHS TLQQMVLENF LQRERTKELQ
DTMSEKGDEI RLKKKVIG
