CCD69_MOUSE
ID CCD69_MOUSE Reviewed; 202 AA.
AC Q3TCJ8; Q8C515;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Coiled-coil domain-containing protein 69;
GN Name=Ccdc69; Synonyms=D11Ertd461e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Egg, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, and Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a scaffold to regulate the recruitment and
CC assembly of spindle midzone components. Required for the localization
CC of AURKB and PLK1 to the spindle midzone.
CC {ECO:0000250|UniProtKB:A6NI79}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:A6NI79}. Midbody {ECO:0000250|UniProtKB:A6NI79}.
CC Note=During early anaphase, localizes along overlapping interpolar
CC microtubules between the separating chromosomes. During late anaphase,
CC localizes to the center of spindle midzone. Concentrated at the midbody
CC during telophase. {ECO:0000250|UniProtKB:A6NI79}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3TCJ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3TCJ8-2; Sequence=VSP_032858;
CC -!- SIMILARITY: Belongs to the CCDC69 family. {ECO:0000305}.
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DR EMBL; AK079810; BAC37752.1; -; mRNA.
DR EMBL; AK135942; BAE22734.1; -; mRNA.
DR EMBL; AK170686; BAE41958.1; -; mRNA.
DR EMBL; BC096763; AAH96763.1; -; mRNA.
DR CCDS; CCDS36155.1; -. [Q3TCJ8-1]
DR RefSeq; NP_803422.2; NM_177471.4. [Q3TCJ8-1]
DR RefSeq; XP_011247427.1; XM_011249125.1. [Q3TCJ8-1]
DR AlphaFoldDB; Q3TCJ8; -.
DR SMR; Q3TCJ8; -.
DR STRING; 10090.ENSMUSP00000054840; -.
DR iPTMnet; Q3TCJ8; -.
DR PhosphoSitePlus; Q3TCJ8; -.
DR SwissPalm; Q3TCJ8; -.
DR EPD; Q3TCJ8; -.
DR jPOST; Q3TCJ8; -.
DR MaxQB; Q3TCJ8; -.
DR PaxDb; Q3TCJ8; -.
DR PRIDE; Q3TCJ8; -.
DR ProteomicsDB; 265594; -. [Q3TCJ8-1]
DR ProteomicsDB; 265595; -. [Q3TCJ8-2]
DR Antibodypedia; 28179; 145 antibodies from 23 providers.
DR Ensembl; ENSMUST00000055040; ENSMUSP00000054840; ENSMUSG00000049588. [Q3TCJ8-1]
DR Ensembl; ENSMUST00000108880; ENSMUSP00000104508; ENSMUSG00000049588. [Q3TCJ8-2]
DR GeneID; 52570; -.
DR KEGG; mmu:52570; -.
DR UCSC; uc007iyu.1; mouse. [Q3TCJ8-1]
DR CTD; 26112; -.
DR MGI; MGI:1196234; Ccdc69.
DR VEuPathDB; HostDB:ENSMUSG00000049588; -.
DR eggNOG; ENOG502RYPP; Eukaryota.
DR GeneTree; ENSGT00950000183026; -.
DR HOGENOM; CLU_1329037_0_0_1; -.
DR InParanoid; Q3TCJ8; -.
DR OMA; ESQNGHC; -.
DR PhylomeDB; Q3TCJ8; -.
DR BioGRID-ORCS; 52570; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Ccdc69; mouse.
DR PRO; PR:Q3TCJ8; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q3TCJ8; protein.
DR Bgee; ENSMUSG00000049588; Expressed in secondary oocyte and 190 other tissues.
DR Genevisible; Q3TCJ8; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051233; C:spindle midzone; ISS:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0051255; P:spindle midzone assembly; ISS:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Lipoprotein;
KW Myristate; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..202
FT /note="Coiled-coil domain-containing protein 69"
FT /id="PRO_0000328963"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 32..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 112..146
FT /evidence="ECO:0000255"
FT MOD_RES 92
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 177..202
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_032858"
SQ SEQUENCE 202 AA; 23296 MW; D1B72A8B391FFC60 CRC64;
MGCRQSRHSR GKRAEKVEET QTELLEALDK EGRILEGRHE EAGQVPQTSN AQEKVSLSDC
IQEAKASLQN TCASHVSPQE ATQAKMNKVD GSILSRLYRN HIQDYGSPGP FWEQELESLH
HVIEMKNERI HELEKQLFLL EMLKEKNLIL ALKNTTLRQE VEDLQFQAGN RLTMSRQLRK
DLLQDLEKES QNGHCCSRRR SH
