CCD86_MOUSE
ID CCD86_MOUSE Reviewed; 426 AA.
AC Q9JJ89; Q8BGH9; Q8C2F2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Coiled-coil domain-containing protein 86;
DE AltName: Full=Cytokine-induced protein with coiled-coil domain;
DE Short=mCyclon;
GN Name=Ccdc86; Synonyms=Cyclon, D19Ertd678e; ORFNames=MNCb-4327;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=17300783; DOI=10.1016/j.febslet.2007.01.083;
RA Hoshino A., Fujii H.;
RT "Redundant promoter elements mediate IL-3-induced expression of a novel
RT cytokine-inducible gene, cyclon.";
RL FEBS Lett. 581:975-980(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.;
RT "Isolation of full-length cDNA clones from mouse brain cDNA library made by
RT oligo-capping method.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Thymus, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-194; SER-225;
RP SER-252; SER-253 AND SER-283, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP CITRULLINATION AT ARG-408.
RX PubMed=24463520; DOI=10.1038/nature12942;
RA Christophorou M.A., Castelo-Branco G., Halley-Stott R.P., Oliveira C.S.,
RA Loos R., Radzisheuskaya A., Mowen K.A., Bertone P., Silva J.C.,
RA Zernicka-Goetz M., Nielsen M.L., Gurdon J.B., Kouzarides T.;
RT "Citrullination regulates pluripotency and histone H1 binding to
RT chromatin.";
RL Nature 507:104-108(2014).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17300783}.
CC -!- TISSUE SPECIFICITY: Highly expressed in testis. Also expressed in
CC heart, liver, kidney. {ECO:0000269|PubMed:17300783}.
CC -!- INDUCTION: By interleukin-3 (IL3). {ECO:0000269|PubMed:17300783}.
CC -!- PTM: Citrullinated by PADI4. {ECO:0000269|PubMed:24463520}.
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DR EMBL; DQ501252; ABF68613.1; -; mRNA.
DR EMBL; AB041663; BAA95108.1; -; mRNA.
DR EMBL; AK078407; BAC37260.1; -; mRNA.
DR EMBL; AK078455; BAC37282.1; -; mRNA.
DR EMBL; AK088727; BAC40530.1; -; mRNA.
DR EMBL; BC043326; AAH43326.1; -; mRNA.
DR CCDS; CCDS29593.1; -.
DR RefSeq; NP_076220.2; NM_023731.3.
DR AlphaFoldDB; Q9JJ89; -.
DR SMR; Q9JJ89; -.
DR BioGRID; 224366; 1.
DR IntAct; Q9JJ89; 1.
DR MINT; Q9JJ89; -.
DR STRING; 10090.ENSMUSP00000025639; -.
DR iPTMnet; Q9JJ89; -.
DR PhosphoSitePlus; Q9JJ89; -.
DR EPD; Q9JJ89; -.
DR jPOST; Q9JJ89; -.
DR MaxQB; Q9JJ89; -.
DR PaxDb; Q9JJ89; -.
DR PeptideAtlas; Q9JJ89; -.
DR PRIDE; Q9JJ89; -.
DR ProteomicsDB; 265600; -.
DR Antibodypedia; 43508; 53 antibodies from 19 providers.
DR DNASU; 108673; -.
DR Ensembl; ENSMUST00000025639; ENSMUSP00000025639; ENSMUSG00000024732.
DR GeneID; 108673; -.
DR KEGG; mmu:108673; -.
DR UCSC; uc008grj.2; mouse.
DR CTD; 79080; -.
DR MGI; MGI:1277220; Ccdc86.
DR VEuPathDB; HostDB:ENSMUSG00000024732; -.
DR eggNOG; KOG4538; Eukaryota.
DR GeneTree; ENSGT00390000017281; -.
DR HOGENOM; CLU_065828_0_0_1; -.
DR InParanoid; Q9JJ89; -.
DR OMA; FSQMLQD; -.
DR OrthoDB; 1470010at2759; -.
DR PhylomeDB; Q9JJ89; -.
DR TreeFam; TF325663; -.
DR BioGRID-ORCS; 108673; 24 hits in 76 CRISPR screens.
DR ChiTaRS; Ccdc86; mouse.
DR PRO; PR:Q9JJ89; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9JJ89; protein.
DR Bgee; ENSMUSG00000024732; Expressed in primitive streak and 242 other tissues.
DR Genevisible; Q9JJ89; MM.
DR GO; GO:0005694; C:chromosome; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR InterPro; IPR026570; CCDC86.
DR InterPro; IPR005579; Cgr1-like.
DR PANTHER; PTHR13557; PTHR13557; 1.
DR Pfam; PF03879; Cgr1; 1.
PE 1: Evidence at protein level;
KW Citrullination; Coiled coil; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..426
FT /note="Coiled-coil domain-containing protein 86"
FT /id="PRO_0000286093"
FT REGION 1..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 346..389
FT /evidence="ECO:0000255"
FT COMPBIAS 31..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..149
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..197
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..324
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..386
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 66
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 70
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 172
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 191
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6F5"
FT MOD_RES 194
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 225
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 252
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 253
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 283
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 408
FT /note="Citrulline"
FT /evidence="ECO:0000269|PubMed:24463520"
FT CONFLICT 1
FT /note="M -> L (in Ref. 2; BAA95108)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="C -> F (in Ref. 3; BAC37282/BAC37260)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 426 AA; 46487 MW; 63A4014176E1F1B7 CRC64;
MDTPLRRSRR LEGLKPLSPE NLPVPEVSRA KRALVDFKSN SEETGELKST RVPPLSLPSP
GPQPETSPGS PCPPLSLPSP GPQPETSPGS PCPPLSLPSP GPQPETSPGS PCPPLSLPSP
GPQPETSPGS PCPPLSLPSP GPQPEASPGS PGPRQDADDG SPQRQPEPHP GSLQPHQDLG
LESPAGQTES SPESPQREQP SKLPPPQGEL DSEAAHAKEE VIPGSPEPCP GQQAPGPEPS
QPAQELTVQA PSSPERQLEP GKLPPAGETV TESLNLKKRV IASPQAPASK KLKEKEELPV
IPKGKPKSGR VWKDRSKKRF SQMVQDKPLR TSWQRKMKER QERKLAKDFA RHLEEEKQRR
RQEKKERRAE NLRRRLENER KAEIVQVIRN PAKLKKAKKK QLRSIEKRDT LALLQKQPPQ
RPVAKV
