CCD87_MOUSE
ID CCD87_MOUSE Reviewed; 855 AA.
AC Q8CDL9; A6H5W2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Coiled-coil domain-containing protein 87;
GN Name=Ccdc87;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=29733332; DOI=10.1093/biolre/ioy106;
RA Wang T., Yin Q., Ma X., Tong M.H., Zhou Y.;
RT "Ccdc87 is critical for sperm function and male fertility.";
RL Biol. Reprod. 99:817-827(2018).
CC -!- FUNCTION: Plays a role in spermatogenesis, where it is important for
CC normal sperm head morphology. Also required for the acrosome reaction
CC and thus normal male fertility. {ECO:0000269|PubMed:29733332}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis (at protein
CC level). Not detected in other tissues tested (at protein level). In the
CC testis, localizes to pachytene spermatocytes and spermatids.
CC {ECO:0000269|PubMed:29733332}.
CC -!- DEVELOPMENTAL STAGE: Detected from postnatal day 14 onwards. Maintained
CC at high levels through to adulthood. {ECO:0000269|PubMed:29733332}.
CC -!- DISRUPTION PHENOTYPE: No gross phenotype. Males have significantly
CC reduced fertility. Testis weight, testis histology and sperm counts are
CC normal. Approximately 25% of sperm have morphological defects in the
CC sperm head and sperm nucleus, while morphology of the sperm flagellum
CC appears normal. Capacitation-induced sperm motility is initially
CC slightly reduced but then recovers. Frequency of both spontaenous and
CC P4-induced acrosome reactions are significantly reduced. In an in-vitro
CC fertilization assay, spermatozoa are able to bind and penetrate the
CC oocyte perivitelline space but fertilizing capacity is severely
CC impaired. {ECO:0000269|PubMed:29733332}.
CC -!- SIMILARITY: Belongs to the CCDC87 family. {ECO:0000305}.
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DR EMBL; AK029877; BAC26655.1; -; mRNA.
DR EMBL; CH466612; EDL33072.1; -; Genomic_DNA.
DR EMBL; BC145660; AAI45661.1; -; mRNA.
DR CCDS; CCDS29439.1; -.
DR RefSeq; NP_997151.2; NM_207268.3.
DR AlphaFoldDB; Q8CDL9; -.
DR BioGRID; 239969; 1.
DR STRING; 10090.ENSMUSP00000086028; -.
DR iPTMnet; Q8CDL9; -.
DR PhosphoSitePlus; Q8CDL9; -.
DR PaxDb; Q8CDL9; -.
DR PRIDE; Q8CDL9; -.
DR ProteomicsDB; 265601; -.
DR Antibodypedia; 44485; 101 antibodies from 15 providers.
DR DNASU; 399599; -.
DR Ensembl; ENSMUST00000088653; ENSMUSP00000086028; ENSMUSG00000067872.
DR GeneID; 399599; -.
DR KEGG; mmu:399599; -.
DR UCSC; uc008gbb.1; mouse.
DR CTD; 55231; -.
DR MGI; MGI:3026882; Ccdc87.
DR VEuPathDB; HostDB:ENSMUSG00000067872; -.
DR eggNOG; ENOG502QQN1; Eukaryota.
DR GeneTree; ENSGT00390000018647; -.
DR HOGENOM; CLU_016540_0_0_1; -.
DR InParanoid; Q8CDL9; -.
DR OMA; WEKWSNK; -.
DR OrthoDB; 365617at2759; -.
DR PhylomeDB; Q8CDL9; -.
DR TreeFam; TF353429; -.
DR BioGRID-ORCS; 399599; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q8CDL9; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q8CDL9; protein.
DR Bgee; ENSMUSG00000067872; Expressed in spermatid and 35 other tissues.
DR Genevisible; Q8CDL9; MM.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:2000344; P:positive regulation of acrosome reaction; IMP:UniProtKB.
DR GO; GO:1905516; P:positive regulation of fertilization; IMP:UniProtKB.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR037383; CCDC87.
DR PANTHER; PTHR16078; PTHR16078; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Differentiation; Fertilization; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..855
FT /note="Coiled-coil domain-containing protein 87"
FT /id="PRO_0000295146"
FT REGION 23..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 387..413
FT /evidence="ECO:0000255"
FT COILED 764..789
FT /evidence="ECO:0000255"
FT COMPBIAS 25..39
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..302
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 246
FT /note="P -> A (in Ref. 1; BAC26655)"
FT /evidence="ECO:0000305"
FT CONFLICT 694
FT /note="L -> V (in Ref. 1; BAC26655)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 855 AA; 98467 MW; C7C805B9A6D5E6B8 CRC64;
MEPHSQEPDL KPIYHRLLSP LSLFPSKAKP PPEPPKRPSQ DATPLQSIPL AKLKVGPLCR
QVSKRLASSG RAARVTAKDR LRLTEVILEE LKCNWREPPI EPILDYENNQ KLRQRLESYV
LISSEQLFVR YLHLLVTLPT SRRVFTESAT LSRLAVNLAR DCTVFLTSPD VYRCLLADFQ
TLLNLKHAQG GIVKLRPPPC PPGTFKLCPI PWPHSTGLDH MPCSSLNLNY LVQLSRPYDF
PSEPEPDPVE ELKSIPQLKS RQQLLWVPSM IKDKEIESRP SPMVPLPSHS PSSESHQFPT
SPVHSWLQRG QSMPCLHEGW SLADELCLLP PSPHPLTPLI LASESKPLPF RDIVAEDLKQ
KMKIMRMEWS RYSLLDSGLP PLLGVLTRRL TAQHHLEKLQ QMIKSLQEEE ASGKWDLQPP
RIIPLHPQPV TVALKVHDQV IVQVATVQLS ERYFNDSFHV EGAGVLYNHL TGELDGKAIE
EMDADRLVGN TTGEVYKELM SRVSVSHLSF EEGDQIEPSA DKDWSSYLAS SFLHQDKHMP
IINRNLVGFY SRRTSTPKPV PEKVPSLTLL PRHKSWDKRP NRHGVWMNWL KPSVSSEDYF
KYLSFQESDF LHVIFQMYEK EAPVEVPVPV QEYLDIQQPP PLLQDEELEF MQGKWDWSSV
IEDGSGPGRA YIHNLQQRLK RLWVMLEVPE QNRLDMVIKY SSNARLQQLP ALIKAWEQVL
KPIQKRESLL GRLEWFEQQA SDPNRFFQKP DLLMNRLLEE NRFRSYLQRK LNRMESNLVS
LLERIESVFG EPVTFKGRSY LKKMKQDKVE MLYWLQQQRR IRNLTQAQKT FRQSCTFTGS
SSQALVAPGN TPTTH
