CCDA_ARATH
ID CCDA_ARATH Reviewed; 354 AA.
AC Q9M5P3; Q8LBH9; Q93Y53;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Cytochrome c-type biogenesis ccda-like chloroplastic protein;
DE AltName: Full=Cytochrome b6f biogenesis protein CCDA;
GN Name=CCDA; OrderedLocusNames=At5g54290; ORFNames=MDK4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10946110; DOI=10.1016/s0300-9084(00)00605-2;
RA Nakamoto S.S., Hamel P., Merchant S.;
RT "Assembly of chloroplast cytochromes b and c.";
RL Biochimie 82:603-614(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND DISRUPTION PHENOTYPE.
RX PubMed=15159384; DOI=10.1074/jbc.m404285200;
RA Page M.L., Hamel P.P., Gabilly S.T., Zegzouti H., Perea J.V., Alonso J.M.,
RA Ecker J.R., Theg S.M., Christensen S.K., Merchant S.;
RT "A homolog of prokaryotic thiol disulfide transporter CcdA is required for
RT the assembly of the cytochrome b6f complex in Arabidopsis chloroplasts.";
RL J. Biol. Chem. 279:32474-32482(2004).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=20214498; DOI=10.1089/ars.2010.3138;
RA Motohashi K., Hisabori T.;
RT "CcdA is a thylakoid membrane protein required for the transfer of reducing
RT equivalents from stroma to thylakoid lumen in the higher plant
RT chloroplast.";
RL Antioxid. Redox Signal. 13:1169-1176(2010).
CC -!- FUNCTION: Required for the transfer of reducing equivalents from stroma
CC to thylakoid lumen. Involved in the biogenesis of the plastid
CC cytochrome b6f complex by probably transferring reducing equivalents
CC from stromal m-type thioredoxin (Trx-m) to the lumenal thioredoxin
CC HCF164. {ECO:0000269|PubMed:15159384, ECO:0000269|PubMed:20214498}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:15159384, ECO:0000269|PubMed:20214498}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:15159384,
CC ECO:0000269|PubMed:20214498}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M5P3-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: High chlorophyll fluorescence and deficiency in
CC the accumulation of the cytochrome b6f complex subunits. Dwarf due to
CC very slow growth and pale green leaves. {ECO:0000269|PubMed:15159384}.
CC -!- SIMILARITY: Belongs to the DsbD family. {ECO:0000305}.
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DR EMBL; AF225913; AAF35369.1; -; mRNA.
DR EMBL; AB010695; BAB10752.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96480.1; -; Genomic_DNA.
DR EMBL; AY050929; AAK93606.1; -; mRNA.
DR EMBL; AY150405; AAN12950.1; -; mRNA.
DR EMBL; AY087199; AAM64755.1; -; mRNA.
DR RefSeq; NP_200240.1; NM_124809.3. [Q9M5P3-1]
DR AlphaFoldDB; Q9M5P3; -.
DR STRING; 3702.AT5G54290.2; -.
DR TCDB; 5.A.1.2.5; the disulfide bond oxidoreductase d (dsbd) family.
DR PaxDb; Q9M5P3; -.
DR PRIDE; Q9M5P3; -.
DR ProteomicsDB; 223874; -. [Q9M5P3-1]
DR EnsemblPlants; AT5G54290.1; AT5G54290.1; AT5G54290. [Q9M5P3-1]
DR GeneID; 835517; -.
DR Gramene; AT5G54290.1; AT5G54290.1; AT5G54290. [Q9M5P3-1]
DR KEGG; ath:AT5G54290; -.
DR Araport; AT5G54290; -.
DR eggNOG; ENOG502QR2K; Eukaryota.
DR HOGENOM; CLU_053225_0_0_1; -.
DR InParanoid; Q9M5P3; -.
DR OMA; LGCFLWG; -.
DR PhylomeDB; Q9M5P3; -.
DR PRO; PR:Q9M5P3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9M5P3; baseline and differential.
DR Genevisible; Q9M5P3; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0055035; C:plastid thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0010190; P:cytochrome b6f complex assembly; IMP:UniProtKB.
DR InterPro; IPR003834; Cyt_c_assmbl_TM_dom.
DR Pfam; PF02683; DsbD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Cytochrome c-type biogenesis;
KW Electron transport; Membrane; Plastid; Reference proteome; Thylakoid;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..354
FT /note="Cytochrome c-type biogenesis ccda-like chloroplastic
FT protein"
FT /id="PRO_0000394554"
FT TOPO_DOM 1..134
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..176
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..206
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..259
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..285
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..321
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..354
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT CONFLICT 23
FT /note="N -> T (in Ref. 5; AAM64755)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="L -> F (in Ref. 5; AAM64755)"
FT /evidence="ECO:0000305"
FT CONFLICT 119
FT /note="E -> A (in Ref. 4; AAK93606)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 36530 MW; 7CB590C09BF0A6FE CRC64;
MNLSVNRCIT GGFVGGFSSC RLNHEKRWVR AGKHCELERE RSLVSDAVSL ERLESKSIKL
AMLASGLGVA NLVTLSSAKA ADLKMIVLDQ ATSIYILAEG SLGDSVGNFL YSANQQANEA
VQDQLSALSV TSLAVIFGAG LVTSLSPCTL SVLPLTLGYI GAFGSGKSRG QVIGDTVAFA
LGLATTLALL GIVASFAGKA YGQIGQGLPV AASGLAIVMG LNLLEIIELQ LPSFFNNFDP
RAAAANFPSS VQAYLAGLTF ALAASPCSTP VLATLLGYVA TSRDPVIGGS LLLTYTTGYV
APLIVAASFA GALQSLLSLR KVSAWINPIS GALLLGGGLY TFLDRLFPAA TMVM
